Results 21 to 30 of about 67,162 (289)
Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity
eLife, 2020 Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical ...
Mark Shaaya +14 more
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Frontiers in Bioengineering and Biotechnology, 2015 Modeling metabolism is fundamental for cell factory design. Central carbon metabolism (CCM) is particularly important as it provides the energy and precursors for other biological processes.
Daniel eMachado +2 more
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Structurally distributed surface sites tune allosteric regulation [PDF]
eLife, 2021 Our ability to rationally optimize allosteric regulation is limited by incomplete knowledge of the mutations that tune allostery. Are these mutations few or abundant, structurally localized or distributed? To examine this, we conducted saturation mutagenesis of a synthetic allosteric switch in which Dihydrofolate reductase (DHFR ...
James W. McCormick +4 more
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Rational engineering of enzyme allosteric regulation through sequence evolution analysis.
PLoS Computational Biology, 2012 Control of enzyme allosteric regulation is required to drive metabolic flux toward desired levels. Although the three-dimensional (3D) structures of many enzyme-ligand complexes are available, it is still difficult to rationally engineer an ...
Jae-Seong Yang +4 more
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Allosteric regulation of rhomboid intramembrane proteolysis [PDF]
The EMBO Journal, 2014 AbstractProteolysis within the lipid bilayer is poorly understood, in particular the regulation of substrate cleavage. Rhomboids are a family of ubiquitous intramembrane serine proteases that harbour a buried active site and are known to cleave transmembrane substrates with broad specificity.
Elena, Arutyunova +5 more
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Functional selectivity of insulin receptor revealed by aptamer-trapped receptor structures
Nature Communications, 2022 DNA aptamers can activate insulin receptor as selective agonists or positive allosteric modulators. Here, authors determine structures of the insulin receptor bound to aptamers and provide a basis for the selective activation or allosteric regulation of ...
Junhong Kim +8 more
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Binding leverage as a molecular basis for allosteric regulation.
PLoS Computational Biology, 2011 Allosteric regulation involves conformational transitions or fluctuations between a few closely related states, caused by the binding of effector molecules.
Simon Mitternacht, Igor N Berezovsky
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Computational modeling of cAMP-dependent protein kinase allostery [PDF]
Proceedings of the Estonian Academy of Sciences, 2023 Allosteric regulation by ATP of peptide binding with a cAMP-dependent protein kinase catalytic subunit was comÂputationally modeled by combining conventional docking analysis and molecular dynamics calculations.
Andrei Izvolski, Aleksei Kuznetsov
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Allosteric regulation of phenylalanine hydroxylase [PDF]
Archives of Biochemistry and Biophysics, 2012 The liver enzyme phenylalanine hydroxylase is responsible for conversion of excess phenylalanine in the diet to tyrosine. Phenylalanine hydroxylase is activated by phenylalanine; this activation is inhibited by the physiological reducing substrate tetrahydrobiopterin.
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Nature Communications, 2021 The allosteric regulation of the bienzyme complex imidazole glycerol phosphate synthase (HisFH) remains to be elucidated. Here, the authors provide structural insights into the dynamic allosteric mechanism by which ligand binding to the cyclase and ...
Jan Philip Wurm +6 more
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