Results 21 to 30 of about 127,473 (347)

Dynamic allosteric control of noncovalent DNA catalysis reactions [PDF]

, 2008
Allosteric modulation of catalysis kinetics is prevalent in proteins and has been rationally designed for ribozymes. Here, we present an allosteric DNA molecule that, in its active configuration, catalyzes a noncovalent DNA reaction.
Winfree, Erik, Zhang, David Yu
core   +1 more source

Functional selectivity of insulin receptor revealed by aptamer-trapped receptor structures

Nature Communications, 2022
DNA aptamers can activate insulin receptor as selective agonists or positive allosteric modulators. Here, authors determine structures of the insulin receptor bound to aptamers and provide a basis for the selective activation or allosteric regulation of ...
Junhong Kim, Na-Oh Yunn, Mangeun Park, Jihan Kim, Seongeun Park, Yoojoong Kim, Jeongeun Noh, Sung Ho Ryu, Yunje Cho   +8 more
doaj   +1 more source

Design of an allosterically regulated retroaldolase [PDF]

Protein Science, 2015
AbstractWe employed a minimalist approach for design of an allosterically controlled retroaldolase. Introduction of a single lysine residue into the nonenzymatic protein calmodulin led to a 15,000‐fold increase in the second order rate constant for retroaldol reaction with methodol as a substrate.
Elizabeth A. Raymond, Yurii S. Moroz, Ivan V. Korendovych, Korrie L. Mack, Jennifer H. Yoon, Olesia V. Moroz   +5 more
openaire   +3 more sources

Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch [PDF]

, 2010
The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation.
F. Bai, R. W. Branch, D. V. Nicolau, T. Pilizota, B. C. Steel, P. K. Maini, R. M. Berry, Monod, Duke, Duke, Changeux, Sowa, Cluzel, Bray, Turner, Alon, Kudo, Block, Scharf, Korobkova, Sowa, Ryu, Korobkova, Lapidus, Block, van Albada, Yuan, Karpen, Dyer, Van Way   +29 more
core   +3 more sources

Allosteres to regulate neurotransmitter sulfonation [PDF]

Journal of Biological Chemistry, 2019
Catecholamine neurotransmitter levels in the synapses of the brain shape human disposition-cognitive flexibility, aggression, depression, and reward seeking-and manipulating these levels is a major objective of the pharmaceutical industry. Certain neurotransmitters are extensively sulfonated and inactivated by human sulfotransferase 1A3 (SULT1A3).
Kristie Darrah, Mary Cacace, Alexander Deiters, Ting Wang, Ian Cook, Thomas S. Leyh   +5 more
openaire   +3 more sources

Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity

eLife, 2020
Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical ...
Mark Shaaya, Jordan Fauser, Anastasia Zhurikhina, Jason E Conage-Pough, Vincent Huyot, Martin Brennan, Cameron T Flower, Jacob Matsche, Shahzeb Khan, Viswanathan Natarajan, Jalees Rehman, Pradeep Kota, Forest M White, Denis Tsygankov, Andrei V Karginov   +14 more
doaj   +1 more source

Allosteric Regulation Alters Carrier Domain Translocation in Pyruvate Carboxylase [PDF]

, 2018
Pyruvate carboxylase (PC) catalyzes the ATP-dependent carboxylation of pyruvate to oxaloacetate. The reaction occurs in two separate catalytic domains, coupled by the long-range translocation of a biotinylated carrier domain (BCCP). Here, we use a series
Budelier, Melissa M., Liu, Yumeng, St. Maurice, Martin, Stine, Katelyn   +3 more
core   +3 more sources

Allosteric Regulation of Human Plastins [PDF]

, 2021
ABSTRACTPlastins/fimbrins are conserved actin-bundling proteins contributing to motility, cytokinesis, and other cellular processes by organizing actin assemblies of strikingly different geometries as in aligned bundles and branched networks. We propose that this unique ability stems from an allosteric communication between plastins’ two actin-binding ...
Christopher L. Schwebach, Elena Kudryashova, Richa Agrawal, Weili Zheng, Edward H. Egelman, Dmitri S. Kudryashov   +5 more
openaire   +2 more sources

Rational engineering of enzyme allosteric regulation through sequence evolution analysis.

PLoS Computational Biology, 2012
Control of enzyme allosteric regulation is required to drive metabolic flux toward desired levels. Although the three-dimensional (3D) structures of many enzyme-ligand complexes are available, it is still difficult to rationally engineer an ...
Jae-Seong Yang, Sang Woo Seo, Sungho Jang, Gyoo Yeol Jung, Sanguk Kim   +4 more
doaj   +1 more source

A high-throughput mass spectrometric assay for discovery of human lipoxygenase inhibitors and allosteric effectors. [PDF]

, 2015
Lipoxygenases (LOXs) regulate inflammation through the production of a variety of molecules whose specific downstream effects are not entirely understood due to the complexity of the inflammation pathway.
Holman, Theodore R, Jameson, J Brian, Kenyon, Victor   +2 more
core   +1 more source