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Epidemiologic, Entomologic, and Virologic Findings during Reemergence of Western Equine Encephalitis Virus, Argentina. [PDF]
Emerg Infect DisSpinsanti L +27 more
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Animal Health Research Reviews, 2012
AbstractThe alphavirus replicon technology has been utilized for many years to develop vaccines for both veterinary and human applications. Many developments have been made to the replicon platform recently, resulting in improved safety and efficacy of replicon particle (RP) vaccines. This review provides a broad overview of the replicon technology and
Ryan L, Vander Veen +2 more
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AbstractThe alphavirus replicon technology has been utilized for many years to develop vaccines for both veterinary and human applications. Many developments have been made to the replicon platform recently, resulting in improved safety and efficacy of replicon particle (RP) vaccines. This review provides a broad overview of the replicon technology and
Ryan L, Vander Veen +2 more
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Alphavirus vectors and vaccination
Reviews in Medical Virology, 2002AbstractAlphaviruses are positive‐stranded RNA viruses that have a broad host range and therefore are capable of replicating in many vertebrate and invertebrate cells. The single‐stranded alphavirus genome is divided into two ORFs. The first ORF encodes the nonstructural proteins that are translated upon entry of the virus into the cytoplasm and are ...
Jonathan O, Rayner +2 more
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Alphavirus Transducing Systems
2008Alphavirus transducing systems (ATSs) are important tools for expressing genes of interest (GOI) in mosquitoes and nonvector insects. ATSs are derived from infectious cDNA clones of mosquito-borne RNA viruses (family Togaviridae). The most common ATSs in use are derived from Sindbis viruses; however, ATSs have been derived from other alphaviruses as ...
Brian D, Foy, Ken E, Olson
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Current Opinion in Biotechnology, 1994
Alphavirus vectors are newcomers in the field of heterologous gene expression. Nevertheless, they have rapidly become popular and are now being used in a wide range of applications. During the past year, new vectors and new methods for their use have improved levels of gene expression.
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Alphavirus vectors are newcomers in the field of heterologous gene expression. Nevertheless, they have rapidly become popular and are now being used in a wide range of applications. During the past year, new vectors and new methods for their use have improved levels of gene expression.
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Purification and Concentration of Alphavirus
Cold Spring Harbor Protocols, 2012The alphaviruses Semliki Forest virus and Sindbis virus have been used frequently as expression vectors in vitro and in vivo. Usually, these systems consist of replication-deficient vectors that require a helper vector for packaging of recombinant particles. Replication-proficient vectors have also been engineered.
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2000
Publisher Summary This chapter describes the mechanics, including how alphaviruses function as expression vectors and some features that make them useful. It also describes some of the ways in which alphaviruses have been used and some potential ways they can be of value. Alphaviruses are positive-strand RNA-enveloped viruses.
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Publisher Summary This chapter describes the mechanics, including how alphaviruses function as expression vectors and some features that make them useful. It also describes some of the ways in which alphaviruses have been used and some potential ways they can be of value. Alphaviruses are positive-strand RNA-enveloped viruses.
openaire +2 more sources