Results 181 to 190 of about 87,684,257 (245)

Exploratory proof-of-concept study and failure mode analysis of a dual-lumen stent graft for transapical off-pump LVAD implantation. [PDF]

Sci Rep
Meissner F, Schön M, Galbas MC, Straky H, Dinkelaker J, Czerny M, Bothe W.   +6 more
europepmc   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

FDA roadmap to reducing animal testing: a regulatory and scientific paradigm shift in nonclinical safety assessment. [PDF]

Stem Cell Res Ther
Lei Y, Tuan RS, Li ZA.
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

The Current Status of the 3Rs Principles in Animal Use in Bosnia and Herzegovina: Overview and Future Perspectives. [PDF]

Animals (Basel)
Smajlhodžić-Deljo M, Pehlivanović Kelle B, Elezović A, Bejdić P, Dervišević A, Kitsara M, Neuhaus W.   +6 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Modelling breeding strategies against genetic defects in a vulnerable cattle breed. [PDF]

PLoS One
Gustafsson E, Johansson AM, Johnsson M.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Acaricide resistance in <i>Amblyomma cajennense</i> s.l. and implications for integrated tick control in Ecuadorian cattle systems. [PDF]

Front Vet Sci
Pérez-Otáñez X, Vanwambeke SO, Paucar-Quishpe V, Pérez C, Grijalva-Olmedo J, Cepeda-Bastidas D, Orozco G, Enríquez Morillo S, Saegerman C, Ron-Garrido L, Rodríguez-Hidalgo R.   +10 more
europepmc   +1 more source