Dermal Permeability of Perfluorohexane Sulfonic Acid (PFHxS) and Perfluorohexanoic Acid (PFHxA) in Varying Vehicles and the Effects on Skin Integrity in a Reconstructed Human Epidermis Model [PDF]
ToxicsPer- and polyfluoroalkyl substances (PFAS) are widespread environmental contaminants associated with adverse health effects, yet dermal exposure remains poorly characterized.
Lisa M. Weatherly +7 more
doaj +3 more sources
SUB6 Subtilisin is Involved During the Initial Adhesion of Trichophyton benhamiae and T. mentagrophytes onto Reconstructed Human Epidermis [PDF]
JID InnovationsThe growing incidence of dermatophytoses and the emergence of strains resistant to available antifungal agents raise the need for a better understanding of the virulence mechanisms of dermatophytes to identify new therapeutic targets.
Émilie Faway +6 more
doaj +3 more sources
Reconstructed Human Epidermis: An Alternative Approach for In Vitro Bioequivalence Testing of Topical Products [PDF]
Pharmaceutics, 2022 The use of in vitro human skin permeation tests is of value when addressing the quality and equivalence of topical drug products in Europe and the US. Human skin is the membrane of choice for these studies. The use of human skin as a membrane is hindered
Ana Sofia Agonia +7 more
doaj +4 more sources
A preclinical model of cutaneous melanoma based on reconstructed human epidermis [PDF]
Scientific Reports, 2022 Malignant melanoma is among the tumor entities with the highest increase of incidence worldwide. To elucidate melanoma progression and develop new effective therapies, rodent models are commonly used.
Anna Leikeim +9 more
doaj +4 more sources
Characterization of Reconstructed Human Epidermis in a Chemically-Defined, Animal Origin-Free Cell Culture [PDF]
JID InnovationsThe Reconstructed Human Epidermis (RHE) model derived from epidermal keratinocytes offers an ethical and scientific alternative to animal experimentation, particularly in cutaneous toxicology and dermatological research, where the elimination of animal ...
Julia Bajsert +6 more
doaj +4 more sources
EpidermaQuant: Unsupervised Detection and Quantification of Epidermal Differentiation Markers on H-DAB-Stained Images of Reconstructed Human Epidermis [PDF]
DiagnosticsThe integrity of the reconstructed human epidermis generated in vitro can be assessed using histological analyses combined with immunohistochemical staining of keratinocyte differentiation markers. Technical differences during the preparation and capture
Dawid Zamojski +3 more
doaj +4 more sources
Evaluation of Skin Irritation of Acids Commonly Used in Cleaners in 3D-Reconstructed Human Epidermis Model, KeraSkinTM [PDF]
Toxics, 2022 Cleaners such as dishwashing liquids contain various chemicals that cause skin damage. Alkaline agents used in cleaners alter the lipid composition of the skin and damage the skin barrier.
Jee-hyun Hwang +4 more
doaj +4 more sources
Harnessing red grape pomace extract for sustainable broad-spectrum photoprotection in a reconstructed human epidermis model [PDF]
Frontiers in PharmacologyRed grape pomace, a major by-product of the winemaking industry, represents a sustainable source of bioactive polyphenols with high nutraceutical and cosmetic potential.
Antonella Smeriglio +10 more
doaj +4 more sources
Visualization of oxygen profile in reconstructed human epidermis by phosphorescence-lifetime imaging microscopy using Ir(III) complex [PDF]
Scientific ReportsReconstructed human epidermis (RHE) is a useful experimental tool for evaluating the effects of various stimuli on the skin. Here, we present a method to visualize the partial pressure of oxygen in RHE with cellular-level spatial resolution by means of ...
Misaki Ochiai-Noguchi +3 more
doaj +4 more sources
Improved Tool for Predicting Skin Irritation on Reconstructed Human Epidermis Models Based on Electrochemical Impedance Spectroscopy [PDF]
Biosensors, 2023 The rabbit skin irritation test has been the standard for evaluating the irritation potential of chemicals; however, alternative methods that do not use animal testing are actively encouraged.
Manuel Chacón +8 more
doaj +4 more sources