A preclinical model of cutaneous melanoma based on reconstructed human epidermis [PDF]
Scientific Reports, 2022 Malignant melanoma is among the tumor entities with the highest increase of incidence worldwide. To elucidate melanoma progression and develop new effective therapies, rodent models are commonly used.
Anna Leikeim +9 more
doaj +5 more sources
Reconstructed Human Epidermis: An Alternative Approach for In Vitro Bioequivalence Testing of Topical Products [PDF]
Pharmaceutics, 2022 The use of in vitro human skin permeation tests is of value when addressing the quality and equivalence of topical drug products in Europe and the US. Human skin is the membrane of choice for these studies. The use of human skin as a membrane is hindered
Ana Sofia Agonia +7 more
doaj +4 more sources
Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation [PDF]
Pharmaceutics, 2020 The development and characterization of reconstructed human epidermis (RHE) is an active area of R&D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages.
Yuri Dancik +6 more
doaj +4 more sources
Improved Tool for Predicting Skin Irritation on Reconstructed Human Epidermis Models Based on Electrochemical Impedance Spectroscopy [PDF]
Biosensors, 2023 The rabbit skin irritation test has been the standard for evaluating the irritation potential of chemicals; however, alternative methods that do not use animal testing are actively encouraged.
Manuel Chacón +8 more
doaj +4 more sources
Evaluation of Skin Irritation of Acids Commonly Used in Cleaners in 3D-Reconstructed Human Epidermis Model, KeraSkinTM [PDF]
Toxics, 2022 Cleaners such as dishwashing liquids contain various chemicals that cause skin damage. Alkaline agents used in cleaners alter the lipid composition of the skin and damage the skin barrier.
Jee-hyun Hwang +4 more
doaj +2 more sources
SUB6 Subtilisin is Involved During the Initial Adhesion of Trichophyton benhamiae and T. mentagrophytes onto Reconstructed Human Epidermis [PDF]
JID InnovationsThe growing incidence of dermatophytoses and the emergence of strains resistant to available antifungal agents raise the need for a better understanding of the virulence mechanisms of dermatophytes to identify new therapeutic targets.
Émilie Faway +6 more
doaj +2 more sources
Characterization of Reconstructed Human Epidermis in a Chemically-Defined, Animal Origin-Free Cell Culture [PDF]
JID InnovationsThe Reconstructed Human Epidermis (RHE) model derived from epidermal keratinocytes offers an ethical and scientific alternative to animal experimentation, particularly in cutaneous toxicology and dermatological research, where the elimination of animal ...
Julia Bajsert +6 more
doaj +2 more sources
A Comprehensive Assessment of the Biocompatibility and Safety of Diamond Nanoparticles on Reconstructed Human Epidermis. [PDF]
Materials (Basel), 2023 Diamond nanoparticles, also known as nanodiamonds (NDs), exhibit remarkable, awe-inspiring properties that make them suitable for various applications in the field of skin care products. However, a comprehensive assessment of their compatibility with human skin, according to the irritation criteria established by the Organization for Economic ...
Fraczek W +6 more
europepmc +3 more sources
EpidermaQuant: Unsupervised Detection and Quantification of Epidermal Differentiation Markers on H-DAB-Stained Images of Reconstructed Human Epidermis [PDF]
DiagnosticsThe integrity of the reconstructed human epidermis generated in vitro can be assessed using histological analyses combined with immunohistochemical staining of keratinocyte differentiation markers. Technical differences during the preparation and capture
Dawid Zamojski +3 more
doaj +2 more sources
Model for human skin reconstructed in vitro composed of associated dermis and epidermis [PDF]
São Paulo Medical Journal, 2006 CONTEXT AND OBJECTIVE: The technique of obtaining human skin with dermis and epidermis reconstructed from cells isolated from patients can enable autologous skin grafting on patients with few donor sites.
Luís Ricardo Martinhão Souto +5 more
doaj +4 more sources