Reconstructed human epidermis: A model to study the barrier function
The use of in vitro reconstructed human epidermis (RHE) by the cosmetic and pharmaceutical industries is increasing because of its similar physiological mechanisms to native human skin. With the advent of ethic laws on animal experimentation, RHE provides an helpful alternative for the test of formulations.
Barbotteau, Y. +12 more
openaire +6 more sources
Bacterial and fungal co-colonization alters <i>Malassezia restricta</i> metabolism and host proteolytic activity in reconstructed human epidermis. [PDF]
Lyou ES +4 more
europepmc +3 more sources
A reproducible mechano-thermal injury method for reconstructed human epidermis to study wound re-epithelialization. [PDF]
Pereira TC +5 more
europepmc +2 more sources
Barrier Function in Reconstructed Epidermis and Its Resemblance to Native Human Skin
One of the prerequisites for the use of human skin equivalents for scientific and screening purposes is that their barrier function is similar to that of native skin. Using human epidermis reconstructed on de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged from ...
Ponec, M. +6 more
openaire +8 more sources
Particulate matter 2.5 induced hyperpigmentation in reconstructed human epidermis model (MelaKutis®) [PDF]
Xiaojing Yang +5 more
doaj +2 more sources
A Comprehensive Comparison of Tissue Processing Methods for High-Quality MALDI Imaging of Lipids in Reconstructed Human Epidermis. [PDF]
Feucherolles M +5 more
europepmc +3 more sources
Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models [PDF]
Atopic dermatitis (AD) is a chronic inflammatory skin disease that can cause skin barrier function damage. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect on deficient skin models, no studies have investigated the effects ...
Tinghan Jia +4 more
doaj +2 more sources
Evaluating blue light impact on reconstructed human epidermis using laser speckle imaging. [PDF]
Habib L +5 more
europepmc +2 more sources
The preparation of a reconstructed human epidermis is described with examples of its utilization in in vitro studies. The model was obtained by culturing normal human keratinocytes at high cell density for 14 days in serum-free and high calcium (1.5 m M) medium on an inert polycarbonate filter at the air-liquid interface.
Poumay, Yves +5 more
openaire +4 more sources
Down-regulation of peptidylarginine deiminase type 1 in reconstructed human epidermis disturbs nucleophagy in the granular layer and affects barrier function. [PDF]
Alioli AC +8 more
europepmc +3 more sources

