Exposure to the anti-microbial chemical triclosan disrupts keratinocyte function and skin integrity in a model of reconstructed human epidermis [PDF]
Journal of Immunotoxicology, 2023 Triclosan is an anti-microbial chemical incorporated into products that are applied to the skin of healthcare workers. Exposure to triclosan has previously been shown to be associated with allergic disease in humans and impact the immune responses in ...
Rachel Baur +5 more
doaj +4 more sources
Model for human skin reconstructed in vitro composed of associated dermis and epidermis [PDF]
São Paulo Medical Journal, 2006 CONTEXT AND OBJECTIVE: The technique of obtaining human skin with dermis and epidermis reconstructed from cells isolated from patients can enable autologous skin grafting on patients with few donor sites.
Luís Ricardo Martinhão Souto +5 more
doaj +5 more sources
A Trichophyton Rubrum Infection Model Based on the Reconstructed Human Epidermis - Episkin® [PDF]
Chinese Medical Journal, 2016 Background: Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood.
Pan-Pan Liang +8 more
doaj +3 more sources
A Comprehensive Assessment of the Biocompatibility and Safety of Diamond Nanoparticles on Reconstructed Human Epidermis. [PDF]
Materials (Basel), 2023 Diamond nanoparticles, also known as nanodiamonds (NDs), exhibit remarkable, awe-inspiring properties that make them suitable for various applications in the field of skin care products. However, a comprehensive assessment of their compatibility with human skin, according to the irritation criteria established by the Organization for Economic ...
Fraczek W +6 more
europepmc +5 more sources
Comparison of structural characteristics and molecular markers of rabbit skin, pig skin, and reconstructed human epidermis for an ex vivo human skin model. [PDF]
Toxicol Res, 2023 Uhm C +5 more
europepmc +5 more sources
Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale [PDF]
Journal of Visualized Experiments, 2021 A three-dimensional human epidermis model reconstructed from neonatal primary keratinocytes is presented. Herein, a protocol for the cultivation process and the characterization of the model is described. Neonatal primary keratinocytes are grown submerged on permeable polycarbonate inserts and lifted to the air-liquid interface three days after seeding.
Dijkhoff, Irini Magdelina +5 more
openaire +5 more sources
Bioactivation of cinnamic alcohol in a reconstructed human epidermis model and evaluation of sensitizing potency of the identified metabolites. [PDF]
Front ToxicolNdreu L +9 more
europepmc +5 more sources
Model of human epidermis reconstructed in vitro with keratinocytes and melanocytes on dead de-epidermized human dermis [PDF]
São Paulo Medical Journal, 2004 CONTEXT: Recent progress in the field of epithelial culture techniques has allowed the development of culture systems in which the reconstructed epidermis presents characteristics of morphological differentiation similar to those seen in vivo.
Jussara Rehder +3 more
doaj +3 more sources
Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation [PDF]
Pharmaceutics, 2020 The development and characterization of reconstructed human epidermis (RHE) is an active area of R&D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages.
Yuri Dancik +6 more
doaj +2 more sources
Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter [PDF]
, 2013 This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions.
De Vuyst, Evelyne +5 more
openaire +4 more sources