A Trichophyton Rubrum Infection Model Based on the Reconstructed Human Epidermis - Episkin® [PDF]
Chinese Medical Journal, 2016 Background: Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood.
Pan-Pan Liang +8 more
doaj +3 more sources
Functional Expression of AQP3 in Human Skin Epidermis and Reconstructed Epidermis [PDF]
Journal of Investigative Dermatology, 2002 The purpose of this study was to examine the presence of aquaporin water channels in human skin and to assess their functional role. On western blots of human epidermis obtained from plastic surgery, a strong signal was obtained with polyclonal anti-aquaporin-3 antibodies.
Sougrat, Rachid +7 more
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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale [PDF]
Journal of Visualized Experiments, 2021 A three-dimensional human epidermis model reconstructed from neonatal primary keratinocytes is presented. Herein, a protocol for the cultivation process and the characterization of the model is described. Neonatal primary keratinocytes are grown submerged on permeable polycarbonate inserts and lifted to the air-liquid interface three days after seeding.
Dijkhoff, Irini Magdelina +5 more
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Human Epidermis Reconstructed by Culture: Is It “Normal”? [PDF]
Journal of Investigative Dermatology, 1986 Human keratinocytes were grown on a dermal equivalent (or lattice) at the liquid-air interface in an attempt to reconstitute a functional epidermis in vitro. Although the multilayered epithelium thus obtained is well differentiated, as shown by the presence of keratohyaline granules and horny layer, several differences from its in vivo counterpart were
Asselineau, Daniel +4 more
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São Paulo Medical JournalCONTEXT: Recent progress in the field of epithelial culture techniques has allowed the development of culture systems in which the reconstructed epidermis presents characteristics of morphological differentiation similar to those seen in vivo.
Jussara Rehder +3 more
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Integration of Langerhans Cells into a Pigmented Reconstructed Human Epidermis [PDF]
Journal of Investigative Dermatology, 1997 The majority of in vitro reconstructed human epidermis is composed of keratinocytes only. Recently, the introduction of melanocytes into epidermal reconstructs has enlarged their field of application. The completion of reconstructed epidermis by introducing Langerhans cells remained an important challenge because Langerhans cells, unlike the other ...
Régnier, Marcelle +3 more
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Exposure to the anti-microbial chemical triclosan disrupts keratinocyte function and skin integrity in a model of reconstructed human epidermis [PDF]
Journal of Immunotoxicology, 2023 Triclosan is an anti-microbial chemical incorporated into products that are applied to the skin of healthcare workers. Exposure to triclosan has previously been shown to be associated with allergic disease in humans and impact the immune responses in ...
Rachel Baur +5 more
doaj +2 more sources
Harnessing red grape pomace extract for sustainable broad-spectrum photoprotection in a reconstructed human epidermis model [PDF]
Frontiers in PharmacologyRed grape pomace, a major by-product of the winemaking industry, represents a sustainable source of bioactive polyphenols with high nutraceutical and cosmetic potential.
Antonella Smeriglio +10 more
doaj +2 more sources
Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis. [PDF]
Ann Dermatol, 2019 Atopic dermatitis (AD) is a chronic disorder, with a vicious cycle of repetitive inflammation and deterioration of the epidermal barrier function. Adiponectin, an adipokine, has anti-inflammatory effects on various metabolic and inflammatory disorders. Recently, its level was found to be reduced in serum and tissue samples from AD patients.We aimed to ...
Seo HS +6 more
europepmc +4 more sources
Visualization of oxygen profile in reconstructed human epidermis by phosphorescence-lifetime imaging microscopy using Ir(III) complex [PDF]
Scientific ReportsReconstructed human epidermis (RHE) is a useful experimental tool for evaluating the effects of various stimuli on the skin. Here, we present a method to visualize the partial pressure of oxygen in RHE with cellular-level spatial resolution by means of ...
Misaki Ochiai-Noguchi +3 more
doaj +2 more sources