Results 11 to 20 of about 5,211 (236)

Phenoxymethylpenicillin amidohydrolases from Penicillium chrysogenum [PDF]

FEBS Letters, 1996
A phenoxymethylpenicillin amidohydrolase which hydrolyses phenoxymethylpenicillin to 6‐aminopenicillanic acid (6‐APA) has been isolated from two species of Penicillium chrysogenum. The amidohydrolase had a molecular mass of approx. 42 kDa. Its activity with benzylpenicillin as substrate was only 1.5% of that with phenoxymethylpenicillin and it was ...
Pat Whiteman, E. P. Abraham
openalex   +4 more sources

Structure of the Ergothioneine‐Biosynthesis Amidohydrolase EgtC [PDF]

ChemBioChem, 2015
AbstractThe ubiquitous sulfur metabolite ergothioneine is biosynthesized by oxidative attachment of a sulfur atom to the imidazole ring of Nα‐trimethylhistidine. Most actinobacteria, including Mycobacterium tuberculosis, use γ‐glutamyl cysteine as a sulfur donor. In subsequent steps the carbon scaffold of γ‐glutamyl cysteine is removed by the glutamine
A. Vit, Gabriel T. Mashabela, Wulf Blankenfeldt, Florian P. Seebeck   +3 more
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Comparative Genomic and Transcriptomic Analysis Reveals Why Paenarthrobacter Strains Are Specialists in the Degradation of the Fungicide Iprodione. [PDF]

Microb Biotechnol
Paenarthrobacter are specialists in degrading the fungicide iprodione. We showed that this trait has evolved through gene duplication of ipaH and ddaH genes, being in the core genome of paenarthrobacters, and further genetic optimisation, with different strains being at different evolution stages depending on their prior exposure to iprodione ...
Michelioudakis V, Zafranas A, Myrisiotis C, Makri S, Katsoula A, Campos M, Vasileiadis S, Karpouzas DG.   +7 more
europepmc   +2 more sources

Effects of pH on the inhibition of fatty acid amidohydrolase by ibuprofen [PDF]

British Journal of Pharmacology, 2001
The pharmacological properties of fatty acid amidohydrolase (FAAH) at different assay pH values were investigated using [3H]‐anandamide ([3H]‐AEA) as substrate in rat brain homogenates and in COS‐7 cells transfected with wild type and mutant FAAH. Rat brain hydrolysis of [3H]‐AEA showed pH dependency with an optimum around pH 8‐9.
Sandra Holt, Jonas Nilsson, Romelda Omeir, Gunnar Tiger, Christopher J. Fowler   +4 more
openalex   +5 more sources

On the Origin of Substrate Specificity of Enzymes from the Amidohydrolase Superfamily. [PDF]

Angew Chem Int Ed Engl
Comprehensive analyses of two enzyme classes from the amidohydrolase superfamily (AHS) revealed that catalysis proceeds either via 1,4 or 1,6 nucleophilic conjugate addition and that this property defines substrate specificity. Moreover, although all substrates and products are entirely achiral, this mechanistic difference results in an inverted ...
Drexler L, F Fürtges T, Rudack T, Sterner R.   +3 more
europepmc   +2 more sources

Thermotolerant D-Hydantoinases for Efficient and Stereoselective Intermediate Synthesis of (R)-3-Isobutylglutaric Acid Monoamide, a Key Intermediate in Pregabalin Production. [PDF]

Microb Biotechnol
We engineered thermo‐alkali‐stable D‐hydantoinases via mutagenesis (M63A/F65H/C317T) to resolve Pregabalin's challenging chiral splitting, achieving ee value > 97% for key intermediate (R)‐3‐isobutylglutaric acid monoamide (R‐IBM) synthesis under extreme conditions (pH 10, 70°C–80°C).
Zhang J, Zhi B, Xie Z, Zhang B, Wang N, Wang W.   +5 more
europepmc   +2 more sources

Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida [PDF]

Journal of Bacteriology, 1987
Formylglutamate amidohydrolase (FGase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolyzed to produce L-glutamate plus formate. Urocanate, the first product in the pathway, induced all five enzymes, but FG was able to induce FGase alone ...
Lan Hu, Lorraine M. Mulfinger, Allen T. Phillips   +2 more
openalex   +4 more sources

Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid [PDF]

, 2003
Acylamidohydrolases from higher plants have not been characterized or cloned so far. AtAMI1 is the first member of this enzyme family from a higher plant and was identified in the genome of Arabidopsis thaliana based on sequence homology with the ...
Andersson, Ausubel, Bar, Barkan, Bartel, Bradford, Chou, Cravatt, Daniel Neu, Davies, Eckhardt, Elmar W Weiler, Hillebrand, Igoshi, Kawaguchi, Kawaguchi, Klee, Klee, Kobayashi, Kobayashi, Kobayashi, Laemmli, LeClere, Magie, Manulis, McKinney, Sambrook, Saotome, Scharf, Schägger, Stephan Pollmann, Takahashi, Towbin, Vorwerk, Weiler, Yamada, Östin   +36 more
core   +2 more sources

Rhodospirillum rubrum L-Asparaginase Conjugates with Polyamines of Improved Biocatalytic Properties as a New Promising Drug for the Treatment of Leukemia

Applied Sciences, 2023
L-asparaginase Rhodospirillum rubrum (RrA) is an enzyme (amidohydrolases; EC 3.5.1.1) that catalyzes the L-asparagine hydrolysis reaction to form L-aspartic acid.
Natalia V. Dobryakova, Dmitry D. Zhdanov, Nikolay N. Sokolov, Svetlana S. Aleksandrova, Marina V. Pokrovskaya, Elena V. Kudryashova   +5 more
doaj   +1 more source