Thermotolerant D-Hydantoinases for Efficient and Stereoselective Intermediate Synthesis of (R)-3-Isobutylglutaric Acid Monoamide, a Key Intermediate in Pregabalin Production. [PDF]
Microb BiotechnolWe engineered thermo‐alkali‐stable D‐hydantoinases via mutagenesis (M63A/F65H/C317T) to resolve Pregabalin's challenging chiral splitting, achieving ee value > 97% for key intermediate (R)‐3‐isobutylglutaric acid monoamide (R‐IBM) synthesis under extreme conditions (pH 10, 70°C–80°C).
Zhang J +5 more
europepmc +2 more sources
Western blot analysis of YqfB and D8_RL. [PDF]
, 2023 Gene-directed enzyme prodrug therapy is an emerging strategy for cancer treatment based on the delivery of a gene that encodes an enzyme that is able to convert a prodrug into a potent cytotoxin exclusively in target cancer cells.
Gediminas Plakys (17508986) +6 more
core +1 more source
Features of phospho- and amidohydrolases functioning in edaphotopes polluted by ore mill effluents
Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ, 2006 Influence of aerotechnogenic contamination of soils on activity of some hydrolytic enzymes of nitrogen and phosphorus cycles is examined. Biochemical mobilization of organophosphorous and nitrogen-bearing compounds in soils polluted by heavy metals is ...
O. M. Artyushenko, V. O. Negulyaev
doaj +1 more source
Biochemical characterization of a new nicotinamidase from an unclassified bacterium thriving in a geothermal water stream microbial mat community. [PDF]
PLoS ONE, 2017 Nicotinamidases are amidohydrolases that convert nicotinamide into nicotinic acid, contributing to NAD+ homeostasis in most organisms. In order to increase the number of nicotinamidases described to date, this manuscript characterizes a nicotinamidase ...
Rubén Zapata-Pérez +5 more
doaj +1 more source
Hormone crosstalk in wound stress response: wound-inducible amidohydrolases can simultaneously regulate jasmonate and auxin homeostasis in Arabidopsis thaliana [PDF]
, 2016 Jasmonate (JA) and auxin are essential hormones in plant development and stress responses. While the two govern distinct physiological processes, their signaling pathways interact at various levels.
Koo, Abraham J +6 more
core +1 more source
Plants, 2014 Amidases [EC 3.5.1.4] capable of converting indole-3-acetamide (IAM) into the major plant growth hormone indole-3-acetic acid (IAA) are assumed to be involved in auxin de novo biosynthesis.
Beatriz Sánchez-Parra +6 more
doaj +1 more source
Purification and Characterization of an ATP‐dependent Amidohydrolase, N‐methylhydantoin Amidohydrolase, from Pseudomonas putida 77 [PDF]
European Journal of Biochemistry, 1995 N‐Methylhydantoin amidohydrolase, an ATP‐dependent amidohydrolase involved in microbial degradation of creatinine, was purified 70‐fold to homogeneity, with a 62% overall recovery, and was crystallized from Pseudomonas putida 77. The enzyme has a relative molecular mass of 300000.
J, Ogawa +5 more
openaire +2 more sources
Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa [PDF]
, 2016 Background: Pseudomonas aeruginosa, a Gram-negative, aerobic coccobacillus bacterium is an opportunistic human pathogen and worldwide the fourth most common cause of hospital-acquired infections which are often high mortality such as ventilator ...
Krämer, Andreas +7 more
core +2 more sources
The Hydrolysis of Amides and the Proficiency of Amidohydrolases. The Burden Borne by kw [PDF]
, 2016 The hydrolysis of small amides has garnered major attention due to its relevance to peptide hydrolysis, one of the most fundamental reactions of biology. Both experimental and theoretical research efforts have studied the reaction in different media, and
Guillermina Estiu (105847) +1 more
core +1 more source
Tackling Critical Catalytic Residues in Helicobacter pylori L-Asparaginase
Biomolecules, 2015 Bacterial asparaginases (amidohydrolases, EC 3.5.1.1) are important enzymes in cancer therapy, especially for Acute Lymphoblastic Leukemia. They are tetrameric enzymes able to catalyze the deamination of L-ASN and, to a variable extent, of L-GLN, on ...
Maristella Maggi +3 more
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