Results 211 to 220 of about 13,885 (249)

Introduction of unnatural amino acids into chalcone isomerase

Bioconjugate Chemistry, 1991
The active site cysteine residue of chalcone isomerase was rapidly and selectively modified under denaturing conditions with a variety of electrophilic reagents. These denatured and modified enzyme were renatured to produce enzyme derivatives containing a series of unnatural amino acids in the active site.
R A, Bednar, C, McCaffrey, K, Shan
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Amino acid sequence of peptidyl‐prolyl isomerase a of fusarium sporotrichioides

Natural Toxins, 1996
AbstractPeptidyl‐prolyl‐cis‐trans isomerase catalyzes the interconversion of the cis and trans isomers of the proline‐containing polypeptides and the folding process of proteins. This protein was known to be cyclophilin which has high binding affinity for cyclosporin A, a cyclic undecapeptide of fungal origin with potent immunosuppressive property ...
L P, Chow, M, Kamo, Y, Ueno, A, Tsugita
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Different contribution of conserved amino acids to the global properties of triosephosphate isomerases

Proteins: Structure, Function, and Bioinformatics, 2013
It is generally assumed that the amino acids that exist in all homologous enzymes correspond to residues that participate in catalysis, or that are essential for folding and stability. Although this holds for catalytic residues, the function of conserved noncatalytic residues is not clear.
Yolanda Aguirre, Nallely Cabrera, Beatriz Aguirre, Ruy Pérez-Montfort, Alejandra Hernandez-Santoyo, Horacio Reyes-Vivas, Sergio Enríquez-Flores, Marietta Tuena de Gómez-Puyou, Armando Gómez-Puyou, Jose M. Sanchez-Ruiz, Miguel Costas   +10 more
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Separation of amino acids in glucose isomerase crystal: Insight from molecular dynamics simulations

Journal of Chromatography A, 2009
The separation of amino acids (Arg, Phe and Trp) in a liquid chromatography is investigated using molecular dynamics simulations. A bioorganic nanoporous material--glucose isomerase crystal--is used as the stationary phase and water as the mobile phase.
Hu, Z., Jiang, J.
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Characterization and isolation of L-to-D-amino-acid-residue isomerase from platypus venom

Amino Acids, 2006
Platypus venom contains an isomerase that reversibly interconverts the second amino-acid residue in some peptides between the L-form and the D-form. The enzyme acts on the natriuretic peptides OvCNPa and OvCNPb, and on the defensin-like peptides DLP-2 and DLP-4, but it does not act on DLP-1. While the isomerization of DLP-2 to DLP-4 is inhibited by the
Torres, Allan M., Tsampazi, M., Kennett, Eleanor C., Belov, Katherine, Geraghty, Dominic P., Bansal, Paramjit S., Alewood, Paul F., Kuchel, Philip W.   +7 more
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Biosynthesis of d-amino acid-containing peptides: Exploring the role of peptide isomerases

1998
The discovery of D-amino acid residues in a growing number of gene-encoded peptides suggests that such biochemical modifications are more common than initially thought. In fact, the extent to which D-amino acids are incorporated into peptides by multicellular organisms probably has not been fully realized, since routine Edman sequencing does not ...
R A, Volkmann, S D, Heck
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Isolation and amino acid sequence analysis of bovine adrenal 3.beta.-hydroxysteroid dehydrogenase/steroid isomerase

Biochemistry, 1991
3 beta-Hydroxysteroid dehydrogenase/steroid isomerase has been purified to homogeneity from bovine adrenal glands. A single protein of molecular weight 42,090 +/- 40 containing both enzyme activities has been isolated. Approximately 86% of the amino acid sequence of the bovine adrenal 3 beta-hydroxysteroid dehydrogenase/steroid isomerase has been ...
K J, Rutherfurd, S A, Chen, J E, Shively
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Gene cloning and amino acid sequence analysis of triose-phosphate isomerase of Schistosoma japonicum Chinese strain

Parasitology International, 1998
A pair of primers was synthesized according to the DNA sequence of Schistosoma japonicum Philippine strain, and the mRNA of adult worms of S. japonicum Chinese strain was prepared. The gene of triose-phosphate isomerase of S. japonicum Chinese strain (SjC TPI) was successfully cloned from the mRNA through reverse transcription polymerase chain reaction
Z, Yinchang, Y, Chuanxin, Y, Xuren, S, Jin   +3 more
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Effect of coordinated addition of specific amino acids on the synthesis of recombinant glucose isomerase

Enzyme and Microbial Technology, 1998
Abstract The amplified expression of a recombinant protein is known to lead to an intracellular depletion of specific amino acid pools which in turn may affect the production of the desired protein. In order to counteract and overcome such a situation during the fermentation of the recombinant Escherichia coli (PMSG27) containing the glucose ...
A Paul, S.H Bhosale, T.K Maity, V.V Deshpande   +3 more
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Site-directed mutagenesis identifies amino acid residues associated with the dehydrogenase and isomerase activities of human type I (placental) 3β-hydroxysteroid dehydrogenase/Isomerase

The Journal of Steroid Biochemistry and Molecular Biology, 1998
3beta-hydroxysteroid dehydrogenase/steroid delta5-->4-isomerase (3beta-HSD/isomerase) was expressed by baculovirus in Spodoptera fungiperda (Sf9) insect cells from cDNA sequences encoding human wild-type I (placental) and the human type I mutants - H261R, Y253F and Y253,254F. Western blots of SDS-polyacrylamide gels showed that the baculovirus-infected
J L, Thomas, B W, Evans, G, Blanco, R W, Mercer, J I, Mason, S, Adler, W E, Nash, K E, Isenberg, R C, Strickler   +8 more
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