The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity [PDF]
Pif1 family DNA helicases are conserved from bacteria to humans and have critical and diverse functions in vivo that promote genome integrity. Pif1 family helicases share a 23 amino acid region, called the Pif1 signature motif (SM) that is unique to this
Galletto, Roberto +3 more
core +2 more sources
Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
A novel member of the Carlavirus genus, provisionally named soybean carlavirus 1 (SCV1), was discovered by RNA-seq analysis of randomly collected soybean leaves in Illinois, USA.
Thanuja Thekke-Veetil +6 more
doaj +1 more source
Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach [PDF]
Chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is composed of two different subunits, GapA and GapB. cDNA clones containing the entire coding sequences of the cytosolic precursors for GapA from pea and for GapB from pea and spinach have ...
F Sanger +35 more
core +1 more source
Two novel mutations of the low density lipoprotein (LDL)-receptor genewere found in two Italian familial hypercholesterolemia (FH)-heterozygotes. Thefirst mutation was an 18 nucleotide duplication in exon 8 which is preceded byan A→T transversion.
Dilip.D. Patel +6 more
doaj +1 more source
Cloning and characterisation of chlorophyll synthase from Avena sativa [PDF]
The chlorophyll synthase gene from oat (Avena sativa) was cloned and expressed in Escherichia coli. The deduced amino acid sequence consists of 378 amino acids including a presequence, of 46 amino acids.
Kögel, Julia +4 more
core +1 more source
Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues.
Zhong Zheng GUI +6 more
doaj +1 more source
Isolation and Identification of a Novel Phlebovirus, Hedi Virus, from Sandflies Collected in China
We report the isolation of a newly recognized phlebovirus, Hedi virus (HEDV), from Phlebotomus chinensis sandflies collected in Shanxi Province, China. The virus’ RNA is comprised of three segments.
Ziqian Xu +7 more
doaj +1 more source
Expression of the hepatitis B surface antigen gene containing the preS2 region in Saccharomyces cerevisiae. [PDF]
We constructed a plasmid, pBH103-ME5, in which the region encoding the 10 preS2 amino acid residues and the S domain of the hepatitis B surface antigen (HBsAg) were regulated by the promoter of the yeast repressible acid phosphatase gene.
Fujita, Hiyoyuki +4 more
core +1 more source
Pancreatic cancer-derived S-100A8 N-terminal peptide: a diabetes cause? [PDF]
BACKGROUND: Our aim was to identify the pancreatic cancer diabetogenic peptide. METHODS: Pancreatic tumor samples from patients with (n=15) or without (n=7) diabetes were compared with 6 non-neoplastic pancreas samples using SDS-PAGE.
BALDO G +12 more
core +1 more source
On the Species Specificity of Acceptor RNA and Attachment Enzymes [PDF]
One of the steps in protein biosynthesis appears to be the attachment of each amino acid to a specific acceptor (SRNA) molecule. According to the adaptor hypothesis, each SRNA molecule would then fit to a specific complementary base sequence on a linear ...
Benzer, Seymour, Weisblum, Bernard
core +2 more sources

