Results 21 to 30 of about 13,598,018 (237)

CHOLESTEROL CRYSTALLIZATION-PROMOTING ACTIVITY OF AMINOPEPTIDASE-N ISOLATED FROM THE VESICULAR CARRIER OF BILIARY LIPIDS

, 1993
Different hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation.
NERVI, F, NUNEZ, L, RIGOTTI, A, PUGLIELLI, L, GRECO, AV, AMIGO, L, MINGRONE, G   +6 more
core   +3 more sources

A Radiochemical Assay for Aminopeptidase N

Analytical Biochemistry, 1993
We developed an assay for aminopeptidase N (AmN) in which substrate, Arg-Phe-[3H]anilide (24.9 Ci/mmol), can be used at concentrations (1-200 nM) well below Km (12 microM) and at or below enzyme concentration ([E]). Such reaction conditions simulate those in vivo where peptide hormones in picomolar concentrations (> [E]. Arg-Phe-[3H]anilide can be used
J W, Ryan, A Y, Chung, J A, Nearing, F A, Valido, C, Shun-Cun, P, Berryer   +5 more
openaire   +2 more sources

Expression of Aminopeptidase N on Human Choriocarcinoma Cells and Cell Growth Suppression by the Inhibition of Aminopeptidase N Activity [PDF]

Japanese Journal of Cancer Research, 1994
We previously found that an aminopeptidase inhibitor, ubenimex (bestatin), had a growth‐suppressive effect on Choriocarcinoma cell lines in vitro. To clarify the mechanism of this action, we investigated the expression of aminopeptidase N (AP‐N/CD13) on Choriocarcinoma cells and other human tumor cells. Two Choriocarcinoma cell lines, NaUCC‐4 and Be Wo,
Ino, Kazuhiko, Goto, Setsuko, Okamoto, Tomomitsu, Nomura, Seiji, Nawa, Akihiro, Isobe, Ken‐ichi, Mizutani, Shigehiko, Tomoda, Yutaka   +7 more
openaire   +2 more sources

Structure and Function of a Multi‐Megadalton Virus‐Like Proteolytic Dodecahedron

Angewandte Chemie, EarlyView.
Zuzalysin, a virulence metallopeptidase of the periodontal pathogen Porphyromonas gingivalis, is secreted as a latent, flexible ≈95‐kDa monomer. Calcium triggers cysteine‐switch activation and hierarchical assembly into pentamers, bipentamers, tripentamers, and a ≈5.6‐MDa, ≈355‐Å dodecahedron. Structures at 1.8–3.6 Å reveal a virus‐sized catalytic cage
Mariusz Madej, Arturo Rodríguez‐Banqueri, Danuta Mizgalska, Borys Szmigielski, Zuzanna Nowakowska, Małgorzata Benedyk‐Machaczka, Monika Bzowska, Katarzyna Mikruta, Juan Sebastián Ramírez‐Larrota, Chinanu Agunanne, Olivier Julien, Anthony J. O'Donoghue, Carsten Scavenius, Mario López‐Martín, Enrique Marcos, Łukasz Koziej, Sebastian Glatt, Pablo Guerra, Ulrich Eckhard, Jan Potempa, F. Xavier Gomis‐Rüth   +20 more
wiley   +2 more sources

The Effect of Different Species Aminopeptidase N Structure on the Activity Screening of Aminopeptidase N Inhibitor

Biological and Pharmaceutical Bulletin, 2010
Aminopeptidase N (APN) is a transmembrane metallopeptidase, which participates in the tumor progress such as proliferation, attachment, angiogenesis and tumor invasion. All of this makes APN as a good chemical therapeutic anti-tumor target. In the present study, we got a novel compound 16l which markedly inhibited the enzyme activity of porcine APN ...
Wang, Xuejian, Zhang, Lei, Yang, Kanghui, Zhang, Cai, Zhang, Jian, Fang, Hao, Xu, Wenfang   +6 more
openaire   +3 more sources

Urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats. [PDF]

PLoS ONE, 2012
This study analyzes the fluorimetric determination of alanyl- (Ala), glutamyl- (Glu), leucyl-cystinyl- (Cys) and aspartyl-aminopeptidase (AspAp) urinary enzymatic activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated ...
Andrés Quesada, Félix Vargas, Sebastián Montoro-Molina, Francisco O'Valle, María Dolores Rodríguez-Martínez, Antonio Osuna, Isabel Prieto, Manuel Ramírez, Rosemary Wangensteen   +8 more
doaj   +1 more source

Further Characterization of Aminopeptidase-N as a Receptor for Coronaviruses [PDF]

, 1994
We recently reported that porcine aminopeptidase-N (pAPN) acts as a receptor for transmissible gastroenteritis virus (TGEV). In the present work, we addressed the question of whether TGEV tropism is determined only by the virus-receptor interaction. To this end, different non-permissive cell lines were transfected with the porcine APN cDNA and tested ...
Delmas, Bernard, Gelfi, Jacqueline, Sjöström, H., Noren, O., Laude, Hubert   +4 more
openaire   +3 more sources

Gene cloning and sequencing of aminopeptidase N3, a putative receptor for Bacillus thuringiensis insecticidal Cry1Ac toxin in Helicoverpa armigera (Lepidoptera: Noctuidae)

European Journal of Entomology, 2005
A cDNA encoding aminopeptidase N3 was cloned by degenerated PCR and RACE techniques. The full-length of APN3harm is 3486bp. Open reading frame is 3042bp in length, encoding 1014 amino acid residues.
Gui-Rong WANG, Ge-Mei LIANG, Kong-Ming WU, Yu-Yuan GUO   +3 more
doaj   +1 more source

Impaired angiogenesis in aminopeptidase N-null mice [PDF]

Proceedings of the National Academy of Sciences, 2007
Aminopeptidase N (APN, CD13; EC 3.4.11.2) is a transmembrane metalloprotease with several functions, depending on the cell type and tissue environment. In tumor vasculature, APN is overexpressed in the endothelium and promotes angiogenesis.
Roberto, Rangel, Yan, Sun, Liliana, Guzman-Rojas, Michael G, Ozawa, Jessica, Sun, Ricardo J, Giordano, Carolyn S, Van Pelt, Peggy T, Tinkey, Richard R, Behringer, Richard L, Sidman, Wadih, Arap, Renata, Pasqualini   +11 more
openaire   +2 more sources

Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection

Emerging Microbes and Infections, 2018
Porcine deltacoronavirus (PDCoV), a member of genus Deltacoronavirus, is an emerging swine enteropathogenic coronavirus (CoV). Although outstanding efforts have led to the identification of Alphacoronavirus and Betacoronavirus receptors, the receptor for
Xinyu Zhu, Shudan Liu, Xunlei Wang, Zhaochen Luo, Yuejun Shi, Dang Wang, Guiqing Peng, Huanchun Chen, Liurong Fang, Shaobo Xiao   +9 more
doaj   +1 more source