Results 91 to 100 of about 238,366 (195)
Use of Whole Genome Phylogeny and Comparisons in the Development of a Multiplex-PCR Assay to Identify Sequence Type 36 Vibrio parahaemolyticus [PDF]
, 2015 Vibrio parahaemolyticus sequence type (ST) 36 strains that are native to the Pacific Ocean have recently caused multi-state outbreaks of gastroenteritis linked to shellfish harvested from the Atlantic Ocean.
Cooper, Vaughn S. +6 more
core +1 more source
Bioinformatic Processing of Amplicon Sequencing Datasets
, 2017 Amplicon sequencing can be a very powerful approach for detecting toxic cyanobacteria or any other kind of microorganism during monitoring programs. However, owing to the huge size of next-generation sequencing (NGS) datasets (up to several Gb), there is an obvious need for semi-automatic data processing and statistical analysis, as well as ...
Sweetlove, Maxime +5 more
openaire +2 more sources
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
BioTechniques, 2019 Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation.
Clarisse Marotz +7 more
doaj +1 more source
Antimicrobial Stewardship & Healthcare EpidemiologyWe compared plasma microbial cell-free DNA sequencing (mcfDNAseq) with an amplicon-based blood next-generation sequencing (NGS) assay for 10 patients with suspected bloodstream or endovascular infection.
Mayar Al Mohajer, Todd Lasco
doaj +1 more source
High-resolution ISR amplicon sequencing reveals personalized oral microbiome
Microbiome, 2018 Background Sequencing of the 16S rRNA gene has been the standard for studying the composition of microbial communities. While it allows identification of bacteria at the level of species, this method does not usually provide sufficient information to ...
Chiranjit Mukherjee +3 more
doaj +1 more source
Quantitative multi-target amplicon sequencing workflow v1
, 2023 High-throughput sequencing of marker gene amplicons is a powerful tool for the discovery of biodiversity, at different levels. However, the method has multiple caveats. Here, we describe in detail a versatile laboratory protocol for high-throughput and cost-effective characterization of microbiota across large numbers of wild insects.
Mateusz Buczek +2 more
openaire +1 more source
Artificial escape from XCI by DNA methylation editing of the CDKL5 gene. [PDF]
, 2020 A significant number of X-linked genes escape from X chromosome inactivation and are associated with a distinct epigenetic signature. One epigenetic modification that strongly correlates with X-escape is reduced DNA methylation in promoter regions. Here,
Anderson, Johnathon D +14 more
core +1 more source
Nanopore amplicon sequencing with DIY adapter v1
, 2023 Nanopore amplicon sequencing with DIY adapter
Jie Hao Ou, Yin-Tse Huang
openaire +1 more source
Frontiers in MicrobiologyMetagenomic studies of the Gouda cheese microbiota and starter cultures are scarce. During the present study, short-read metagenomic sequencing (Illumina) was applied on 89 Gouda cheese and processed milk samples, which have been investigated before ...
Hannes Decadt +5 more
doaj +1 more source
Frontiers in MicrobiologyInfectious salmon anemia (ISA) is an infectious disease primarily affecting farmed Atlantic salmon, Salmo salar, which is caused by the ISA virus (ISAV). ISAV belongs to the Orthomyxoviridae family. The disease is a serious condition resulting in reduced
Bjørn Spilsberg +8 more
doaj +1 more source