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Amplicon Sequencing Pipelines in Metagenomics
2023Taxonomic profiling among a large number of samples is a fundamental task during amplicon sequencing analysis. The heterogeneity and technical noises in the sample handling, library preparation, and sequencing present a major challenge to how the biological conclusions are drawn from the data analysis, and accordingly, many tools have been developed to
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Practical innovations for high-throughput amplicon sequencing
Nature Methods, 2013We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide.
Derek S, Lundberg +4 more
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Amplicon sequence data of locust
2022Changes of microbial community in migratory locusts after Beauveria bassiana infection.
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16S rRNA Amplicon Sequencing for Metagenomics
2018The 16S rRNA amplicon sequencing technique is a microbiome analysis where different samples are analyzed at the same time using multiplexing. The results can be used to evaluate microbial diversity at genus, family, order, class, and phylum levels. The resolution is normally insufficient to evaluate the species level.
Henrik Christensen +3 more
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Characterization of Eukaryotic Microbiome Using 18S Amplicon Sequencing
2018With the advent of low-cost, high-throughput sequencing, taxonomic profiling of complex microbial communities through 16S rRNA marker gene surveys has received widespread interest, uncovering a wealth of information concerning the bacterial composition of microbial communities, as well as their association with health and disease.
Ana, Popovic, John, Parkinson
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SUNFLOWER GENOTYPING USING OXFORD NANOPORE AMPLICON SEQUENCING
Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии, 2022Россия является одной из лидирующих стран по производству подсолнечника, получая порядка 15,3 млн. тон в год с площади 10033 тыс. га (1). Признаки, по которым ведется селекция подсолнечника и улучшение его агрокультурных качеств, сильно разграничены в зависимости от производственных задач.
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Targeted amplicon sequencing for obtaining novel antimicrobial peptide sequences
2017AMPs are endogenous antibiotics present in all organisms acting directly towards pathogens and also showing immunomodulatory properties – altering gene expression to facilitate and modulate immune response1, 2. Skin secretions of many tested anurans have been shown to contain a variety of bioactive peptides that often exhibit antibacterial activity ...
Rončević, Tomislav +3 more
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Data Standards for Multiplex Amplicon Sequencing
Talk on Data Standards for Multiplex Amplicon Sequencing.openaire +1 more source
Exploring Microbial Communities Through Amplicon Sequencing
A presentation where the theory of amplicon sequencing is introduced.openaire +1 more source