Ecological and Genomic Attributes of Novel Bacterial Taxa That Thrive in Subsurface Soil Horizons. [PDF]
, 2019 While most bacterial and archaeal taxa living in surface soils remain undescribed, this problem is exacerbated in deeper soils, owing to the unique oligotrophic conditions found in the subsurface.
Arogyaswamy, Keshav +24 more
core +2 more sources
Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]
, 2020 Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J. +8 more
core +2 more sources
Comprehensive Genome Sequence Analysis of a Breast Cancer Amplicon [PDF]
Genome Research, 2001 Gene amplification occurs in most solid tumors and is associated with poor prognosis. Amplification of 20q13.2 is common to several tumor types including breast cancer. The 1 Mb of sequence spanning the 20q13.2 breast cancer amplicon is one of the most exhaustively studied segments of the human genome.
Collins, Colin +12 more
openaire +3 more sources
Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing
Malaria Journal, 2019 Background Deep sequencing of targeted genomic regions is becoming a common tool for understanding the dynamics and complexity of Plasmodium infections, but its lower limit of detection is currently unknown.
Angela M. Early +7 more
doaj +1 more source
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices [PDF]
, 2015 Background: In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrices, two bidirectional DNA walking ...
De Loose, Marc +6 more
core +6 more sources
Frontiers in Microbiology, 2020 Viable pathogenic bacteria are major biohazards that pose a significant threat to food safety. Despite the recent developments in detection platforms, multiplex identification of viable pathogens in food remains a major challenge.
Manyun Yang +13 more
doaj +1 more source
Self-reinoculation with fecal flora changes microbiota density and composition leading to an altered bile-acid profile in the mouse small intestine [PDF]
, 2019 Background: The upper gastrointestinal tract plays a prominent role in human physiology as the primary site for enzymatic digestion and nutrient absorption, immune sampling, and drug uptake.
Bogatyrev, Said R. +2 more
core +1 more source
Scientific Reports, 2021 High-throughput sequencing platforms are increasingly being used for targeted amplicon sequencing because they enable cost-effective sequencing of large sample sets.
Yasemin Guenay-Greunke +3 more
doaj +1 more source
The unseen world: environmental microbial sequencing and identification methods for ecologists [PDF]
, 2014 Archaea, bacteria, microeukaryotes, and the viruses that infect them (collectively “microorganisms”) are foundational components of all ecosystems, inhabiting almost every imaginable environment and comprising the majority of the planet’s organismal and ...
Aronson, Emma +4 more
core +1 more source
Target enrichment using parallel nanoliter quantitative PCR amplification [PDF]
, 2014 Background: Next generation targeted resequencing is replacing Sanger sequencing at high pace in routine genetic diagnosis. The need for well validated, high quality enrichment platforms to complement the bench-top next generation sequencing devices is ...
De Wilde, Bram +7 more
core +2 more sources