Results 41 to 50 of about 1,025,542 (289)

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Improving product quality and productivity of an antibody-based biotherapeutic using inverted frustoconical shaking bioreactors

Frontiers in Bioengineering and Biotechnology
The Chinese hamster ovarian (CHO) cells serve as a common choice in biopharmaceutical production, traditionally cultivated in stirred tank bioreactors (STRs).
Xuekun Wang, Xuekun Wang, Jin Xu, Jin Xu, Jin Xu, Qingcheng Guo, Qingcheng Guo, Qingcheng Guo, Qingcheng Guo, Zhenhua Li, Zhenhua Li, Zhenhua Li, Jiawei Cao, Jiawei Cao, Jiawei Cao, Rongrong Fu, Rongrong Fu, Mengjiao Xu, Mengjiao Xu, Xiang Zhao, Xiang Zhao, Fugui Wang, Fugui Wang, Xinmeng Zhang, Taimin Dong, Xu Li, Weizhu Qian, Weizhu Qian, Weizhu Qian, Shen Hou, Shen Hou, Shen Hou, Lusha Ji, Lusha Ji, Lusha Ji, Dapeng Zhang, Dapeng Zhang, Dapeng Zhang, Huaizu Guo, Huaizu Guo, Huaizu Guo, Huaizu Guo   +41 more
doaj   +1 more source

Characterization of Norwalk virus GI specific monoclonal antibodies generated against Escherichia coli expressed capsid protein and the reactivity of two broadly reactive monoclonal antibodies generated against GII capsid towards GI recombinant fragments

BMC Microbiology, 2001
Background Norwalk virus causes outbreaks of acute non-bacterial gastroenteritis in humans. The virus capsid is composed of a single 60 kDa protein. In a previous study, the capsid protein of recombinant Norwalk virus genogroup II was expressed in an E ...
Takino Koichi, Utagawa Etsuko, Kawamoto Hiroyoshi, Kuzuguchi Tsuyoshi, Oishi Isao, Yamazaki Kenji, Suzuki Yasuhiko, Terano Yoshitake, Yoda Tomoko, Oda Hajime, Shibata Tadayoshi   +10 more
doaj   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Methodological validation study of nrCE-SDS for monoclonal antibodies

生物医学转化
Objective To perform methodological validation of non-reduced capillary electrophoresis-sodium dodecyl sulfate (nrCE-SDS) for monoclonal antibodies, providing a reference for the methodological validation of corresponding quality control methods in the ...
Xu Gangling, Ni Yongbo, Wu Gang, Fu Zhihao, Yu Chuanfei, Wang Lan   +5 more
doaj   +1 more source

PCSK9 monoclonal antibodies: An overview

Heart Views, 2020
PCSK9 monoclonal antibodies are novel lipid-lowering therapy that have been extensively studied in patients with hypercholesterolemia either as monotherapy or as an add-on to other LLTs.
Rasha Kaddoura, Bassant Orabi, Amar M Salam   +2 more
doaj   +1 more source

Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. [PDF]

, 1985
Three monoclonal antibodies that react with previously undefined antigenic determinants on the clathrin molecule have been produced and characterized. They were isolated from a fusion between myeloma cells and popliteal lymphocytes from SJL mice that had
Brodsky, Frances
core  

Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]

, 2020
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F., Hunka, Julia, Riley, John T   +2 more
core   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source