Results 11 to 20 of about 1,768,583 (320)

Antibody-Producing Cells in Rabbits Injected with Soluble BSA [PDF]

The Journal of Immunology, 1968
Summary A hemolytic plaque technique has been modified for detecting antibody-producing cells in lymphoid tissues of rabbits injected with soluble BSA. A soluble carbodiimide was used to couple BSA to either heterologous (goat) or homologous erythrocytes. In the latter case, background activity was completely eliminated.
J C Daniels, William O Weigle
openaire   +2 more sources

ANTIBODY‐PRODUCING CELLS: ANALYSIS AND PURIFICATION BY VELOCITY SEDIMENTATION

Cell Proliferation, 1970
ABSTRACTVelocity sedimentation cell separation is a simple and reproducible method for obtaining highly enriched populations of viable antibody‐producing cells. Using suspensions of spleen cells prepared from mice immunized with sheep erythrocytes, fractions containing up to 2% 19S‐PFC and 25% 7S‐PFC can be obtained.
R. A. Phillips, R. G. Miller
openaire   +4 more sources

A New Method for the Enumeration of Single Antibody-Producing Cells

The Journal of Immunology, 1968
Summary A new method is described for the enumeration of single cells producing antibody against a pure protein preparation from Salmonella adelaide flagella. Cells with adherent bacteria, cultured in nutrient agar, give rise to bacteria adherence colonies which can be counted under low power magnification.
E. Diener
openaire   +3 more sources

Demonstration That Antigen-Binding Cells Are Precursors of Antibody-Producing Cells After Purification with a Fluorescence-Activated Cell Sorter

Proceedings of the National Academy of Sciences of the United States of America, 1972
Michael H. Julius, Takeshi Masuda, L A Herzenberg   +2 more
openalex   +2 more sources

FK506-Binding Protein 11 Is a Novel Plasma Cell-Specific Antibody Folding Catalyst with Increased Expression in Idiopathic Pulmonary Fibrosis

Cells, 2022
Antibodies are central effectors of the adaptive immune response, widespread used therapeutics, but also potentially disease-causing biomolecules. Antibody folding catalysts in the plasma cell are incompletely defined. Idiopathic pulmonary fibrosis (IPF)
Stefan Preisendörfer, Yoshihiro Ishikawa, Elisabeth Hennen, Stephan Winklmeier, Jonas C. Schupp, Larissa Knüppel, Isis E. Fernandez, Leonhard Binzenhöfer, Andrew Flatley, Brenda M. Juan-Guardela, Clemens Ruppert, Andreas Guenther, Marion Frankenberger, Rudolf A. Hatz, Nikolaus Kneidinger, Jürgen Behr, Regina Feederle, Aloys Schepers, Anne Hilgendorff, Naftali Kaminski, Edgar Meinl, Hans Peter Bächinger, Oliver Eickelberg, Claudia A. Staab-Weijnitz   +23 more
doaj   +1 more source

The immunosenescence-related factor DOCK11 is involved in secondary immune responses of B cells

Immunity & Ageing, 2022
Background Memory B cells are an antigen-experienced B-cell population with the ability to rapidly differentiate into antibody-producing cells by recall responses.
Yuma Sugiyama, Mitsuhiro Fujiwara, Akihiko Sakamoto, Hiromichi Tsushima, Akihiko Nishikimi, Mitsuo Maruyama   +5 more
doaj   +1 more source

Detection of circulating B cells producing anti-GPIb autoantibodies in patients with immune thrombocytopenia. [PDF]

PLoS ONE, 2014
BACKGROUND: We previously reported that an enzyme-linked immunospot (ELISPOT) assay for detecting anti-GPIIb/IIIa antibody-secreting B cells is a sensitive method for identifying patients with immune thrombocytopenia (ITP).
Masataka Kuwana, Yuka Okazaki, Yasuo Ikeda   +2 more
doaj   +1 more source

Immunohistochemical detection of co-localizing cytokine and antibody producing cells in the extrafollicular area of human palatine tonsils

Clinical and Experimental Immunology, 1993
Saskia Hoefakker, E. H. M. van't Erve, C. Deen, Alfons J.M. van den Eertwegh, W.J.A. Boersma, W.R.F. Notten, Eric Claassen   +6 more
openalex   +3 more sources

Introduction of direct-acting antiviral agents alters frequencies of anti-GPIIb/IIIa antibody-producing B cells in chronic hepatitis C patients with thrombocytopenia

Platelets, 2023
The pathogenesis of thrombocytopenia in chronic hepatitis C (CHC) conceivably involves autoimmunity; however, the dynamics of autoantibodies and other autoimmune mechanisms remain unclear.
Takashi Satoh, Haruki Uojima, Naohisa Wada, Hayato Takiguchi, Mei Kaneko, Marina Nakamura, Natsuki Gonda, Michika Homma, Hisashi Hidaka, Chika Kusano, Ryouichi Horie   +10 more
doaj   +1 more source

High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay

iScience, 2022
Summary: High-throughput screening and enrichment of antibody-producing cells have many important applications. Herein, we present a droplet microfluidic approach for high-throughput screening and sorting of antibody-secreting cells using a Förster ...
Justina Rutkauskaite, Simon Berger, Stavros Stavrakis, Oliver Dressler, John Heyman, Xavier Casadevall i Solvas, Andrew deMello, Linas Mazutis   +7 more
doaj   +1 more source