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Affinity screening for weak monoclonal antibodies
Journal of Immunological Methods, 1998When selecting for monoclonal antibodies of a desired affinity, affinity chromatography can be a feasible alternative. This is of particular interest when low affinity monoclonal antibodies (dissociation constant (Kd) > 10(-4) M) are screened, as they are not easily recognised by traditional immunoassay procedures.
L, Leickt, A, Grubb, S, Ohlson
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Affinity chromatographic purification of morphine antibody
Life Sciences, 1976Abstract Morphine-6-hemisuccinate was synthesized and linked to agarose affinity beads by either direct amide bond formation or by an N-hydroxysuccinimide ester intermediate using various conditions. The various preparative routes resulted in differing ampunts of covalently bound ligand.
J L, Spratt, S B, Jones
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Purification of antibodies by affinity chromatography
Journal of Biochemical and Biophysical Methods, 2002This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody.
Klaus, Huse +2 more
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Affinity of IgM and IgG antibodies
Immunochemistry, 1970Abstract Immunoglobulins M and G were prepared from a single bleeding from individual rabbits after an injection of a hapten-protein conjugate (nitro-iodo-oxyphenyl acetic acid and chicken globulin, NIP11CG). The concentration of anti-NIP in the preparations was determined by quantitative precipitation.
O, Mäkelä +2 more
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Affinity-labeling of anti-lactose antibody
Immunochemistry, 1975Abstract Purified anti-lactose antibodies induced in the horse and rabbit with a bacterial vaccine have been subjected to affinity-labeling with two bromoacetyl derivatives of lactose-containing ligands. The synthesis of these new radioactive reagents is described and the optimum conditions for the labeling reaction given.
P V, Gopalakrishnan, F, Karush
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Purification of Antibodies Using Affinity Chromatography
2010Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilised to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affinity pair, such as those between an antibody and its ...
Elaine, Darcy +4 more
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Antibody Affinity Maturation by Computational Design
2018The immune systems protect our bodies from foreign molecules or antigens, where antibodies play important roles. Antibodies evolve over time upon antigen encounter by somatically mutating their genome sequences. The end result is a series of antibodies that display higher affinities and specificities to specific antigens.
Daisuke, Kuroda, Kouhei, Tsumoto
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The modification of affinity labeled antibodies
Immunochemistry, 1973Abstract We previously showed that antibodies which had been affinity labeled at a tyrosyl residue with a diazonium labeling reagent could be further modified usefully. By reductively cleaving the azotyrosine bond, 3-aminotyrosine (3-NH2Tyr) was generated in situ, and this residue could then serve as an initiation point for further modifications ...
N, Hadler, H, Metzger
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Antibodies with Infinite Affinity: Origins and Applications
Accounts of Chemical Research, 2006AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Nathaniel G, Butlin, Claude F, Meares
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Purification of Antibodies Using Affinity Chromatography
2016Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilized to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affinity pair, such as those between an antibody and its ...
Elaine, Darcy +5 more
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