Scientific Reports, 2021 Small antibody mimetics that contain high-affinity target-binding peptides can be lower cost alternatives to monoclonal antibodies (mAbs). We have recently developed a method to create small antibody mimetics called FLuctuation-regulated Affinity ...
Kyra See +11 more
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Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust [PDF]
, 2016 A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON.
De Saeger, Sarah +7 more
core +3 more sources
PLoS Computational Biology, 2019 Antibodies developed for research and clinical applications may exhibit suboptimal stability, expressibility, or affinity. Existing optimization strategies focus on surface mutations, whereas natural affinity maturation also introduces mutations in the ...
Shira Warszawski +13 more
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Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis
Frontiers in Immunology, 2017 The identification of mutations that enhance antibody affinity while maintaining high antibody specificity and stability is a time-consuming and laborious process.
Kathryn E. Tiller +6 more
doaj +1 more source
Generation and in vivo characterization of a chimeric αvβ5-targeting antibody 14C5 and its derivatives [PDF]
, 2013 Background: Previous studies showed that radiolabeled murine monoclonal antibody (mAb) 14C5 and its Fab and F(ab')2 fragments, targeting αvβ5 integrin, have promising properties for diagnostic and therapeutic applications in cancer.
De Vos, Filip +5 more
core +2 more sources
A combined computational-experimental approach to define the structural origin of antibody recognition of sialyl-Tn, a tumor-associated carbohydrate antigen. [PDF]
, 2018 Anti-carbohydrate monoclonal antibodies (mAbs) hold great promise as cancer therapeutics and diagnostics. However, their specificity can be mixed, and detailed characterization is problematic, because antibody-glycan complexes are challenging to ...
Amon, Ron +12 more
core +2 more sources
Antibody-based affinity cryo-EM grid [PDF]
Methods, 2016 The Affinity Grid technique combines sample purification and cryo-Electron Microscopy (cryo-EM) grid preparation into a single step. Several types of affinity surfaces, including functionalized lipids monolayers, streptavidin 2D crystals, and covalently functionalized carbon surfaces have been reported.
Guimei Yu, Kunpeng Li, Wen Jiang
openaire +2 more sources
Antibody engineering using phage display with a coiled-coil heterodimeric Fv antibody fragment. [PDF]
PLoS ONE, 2011 A Fab-like antibody binding unit, ccFv, in which a pair of heterodimeric coiled-coil domains was fused to V(H) and V(L) for Fv stabilization, was constructed for an anti-VEGF antibody.
Xinwei Wang +3 more
doaj +1 more source
Bidirectional optimization of the melting spinning process [PDF]
, 2014 This is the author's accepted manuscript (under the provisional title "Bi-directional optimization of the melting spinning process with an immune-enhanced neural network"). The final published article is available from the link below.
Ding, Y +5 more
core +1 more source
OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes. [PDF]
PLoS ONE, 2014 Antibody-based therapeutics provides novel and efficacious treatments for a number of diseases. Traditional experimental approaches for designing therapeutic antibodies rely on raising antibodies against a target antigen in an immunized animal or ...
Tong Li +2 more
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