Results 31 to 40 of about 849,234 (288)

Diphthamide synthesis is linked to the eEF2‐client chaperone machinery

FEBS Letters, EarlyView.
The diphthamide modification of eukaryotic translation elongation factor 2 (eEF2) is important for accurate protein synthesis. We addressed the potential coordination of de novo eEF2 synthesis with simultaneous or subsequent diphthamide modification. Our work reveals that the co‐chaperones Hgh1 and Cpr7, which are known to support folding of nascent ...
Lars Kaduhr, Klaus Mayer, Raffael Schaffrath, Johannes Buchner, Ulrich Brinkmann   +4 more
wiley   +1 more source

Incorporating Pre-training Paradigm for Antibody Sequence-Structure Co-design [PDF]

arXiv, 2022
Antibodies are versatile proteins that can bind to pathogens and provide effective protection for human body. Recently, deep learning-based computational antibody design has attracted popular attention since it automatically mines the antibody patterns from data that could be complementary to human experiences.
arxiv  

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

FEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley, Daniel H. Shain, Eric A. Klein   +2 more
wiley   +1 more source

Specificity and application of SOX2 antibody

Poultry Science, 2020
Sox2 is known to play an important role in maintaining the totipotency and self-renewal of embryonic stem cells. The purpose of this study was to prepare an anti-chicken Sox2 polyclonal antibody using prokaryotic expression techniques, to evaluate its ...
Longqin Wei, Lixia Wang, Jianhong Pan, Lingkang Liu, Pengxia Wang, Jinyu Wei, Xiaoming Xu, Qingbo Xing, Ping Liu, Wende Wu, Gonghe Li, Xibang Zheng   +11 more
doaj  

Specificity and overlap in gene segment-defined antibody repertoires

BMC Genomics, 2005
Background To date several studies have sought to catalog the full suite of antibodies that humans naturally produce against single antigens or other specificities (repertoire).
Arnaout Ramy A
doaj   +1 more source

Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology

Antibodies, 2022
Combinatorial biology methods such as phage and yeast display, suitable for the generation and screening of huge numbers of protein fragments and mutated variants, have been useful when dissecting the molecular details of the interactions between ...
Gertrudis Rojas
doaj   +1 more source

Ablation of LRP6 in alpha‐smooth muscle actin‐expressing cells abrogates lung inflammation and fibrosis upon bleomycin‐induced lung injury

FEBS Letters, EarlyView.
Low‐density lipoprotein receptor‐related protein 6 (LRP6) is a key receptor for the Wnt antagonist Dickkopf1 (DKK1). DKK1 protein expression is induced in a bleomycin (BLM)‐induced lung injury model. We show that DKK1 induces proinflammatory and profibrotic genes in lung fibroblasts.
Eun‐Ah Sung, Mikhail G. Dozmorov, SuJeong Song, Theingi Aung, Min Hee Park, Patricia J. Sime, Wook‐Jin Chae   +6 more
wiley   +1 more source

Accurate Prediction of Antibody Function and Structure Using Bio-Inspired Antibody Language Model [PDF]

arXiv, 2023
In recent decades, antibodies have emerged as indispensable therapeutics for combating diseases, particularly viral infections. However, their development has been hindered by limited structural information and labor-intensive engineering processes. Fortunately, significant advancements in deep learning methods have facilitated the precise prediction ...
arxiv  

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

The solution supramolecular structure of α2 → 8 polysialic acid suggests a structural cause for its low immunogenicity

FEBS Letters, EarlyView.
α2 → 8 polysialic acid elicits poor immunogenicity. Small‐angle scattering shows a supramolecular structure with parallel‐chain binding, although in different forms at μm and mm calcium. The major histocompatibility complex requires molecular weights around 2000 Da to produce antibodies, and 2000 Da polysialic oligomers will bind in these structures ...
Kenneth A. Rubinson
wiley   +1 more source