Results 11 to 20 of about 1,290,412 (287)

NbX: Machine Learning-Guided Re-Ranking of Nanobody–Antigen Binding Poses

Pharmaceuticals, 2021
Modeling the binding pose of an antibody is a prerequisite to structure-based affinity maturation and design. Without knowing a reliable binding pose, the subsequent structural simulation is largely futile.
Chunlai Tam, Ashutosh Kumar, Kam Y. J. Zhang   +2 more
doaj   +1 more source

Identification of critical chemical modifications and paratope mapping by size exclusion chromatography of stressed antibody-target complexes

mAbs, 2021
Therapeutic proteins including antibodies and Fc-fusion proteins undergo a large number of chemical modifications during cell culture, purification, storage and in human circulation.
Pavel V. Bondarenko, Andrew C. Nichols, Gang Xiao, Rachel Liuqing Shi, Pik Kay Chan, Thomas M Dillon, Fernando Garces, David J. Semin, Margaret S. Ricci   +8 more
doaj   +1 more source

Design of Antibody-Functionalized Polymeric Membranes for the Immunoisolation of Pancreatic Islets

Applied Sciences, 2020
An immunoencapsulation strategy for pancreatic islets aimed to reduce the risk of rejection in transplanted patients due to the immune response of the host organism is proposed.
Anna Cavallo, Ugo Masullo, Alessandra Quarta, Alessandro Sannino, Amilcare Barca, Tiziano Verri, Marta Madaghiele, Laura Blasi   +7 more
doaj   +1 more source

Quantitative Description of Surface Complementarity of Antibody-Antigen Interfaces

Frontiers in Molecular Biosciences, 2021
Antibodies have the remarkable ability to recognise their cognate antigens with extraordinary affinity and specificity. Discerning the rules that define antibody-antigen recognition is a fundamental step in the rational design and engineering of ...
Lorenzo Di Rienzo, Edoardo Milanetti, Edoardo Milanetti, Giancarlo Ruocco, Giancarlo Ruocco, Rosalba Lepore   +5 more
doaj   +1 more source

Surface Properties of Antigen–Antibody Complexes [PDF]

Scandinavian Journal of Immunology, 1997
In this paper, the authors show that liquid–liquid partition chromatography in an aqueous two‐phase system offers unique possibilities of comparing the overall surface properties of intact antibodies in solution before and after binding of antigen. The authors demonstrate that the surface properties of antigen–antibody complexes are dependent on the ...
C, Wingren, U B, Hansson
openaire   +2 more sources

pH-Regulated Strategy and Mechanism of Antibody Orientation on Magnetic Beads for Improving Capture Performance of Staphylococcus Species

Foods, 2022
Immunomagnetic beads (IMBs) have been widely used to capture and isolate target pathogens from complex food samples. The orientation of the antibody immobilized on the surface of magnetic beads (MBs) is closely related to the effective recognition with ...
Fuying Kang, Yin Yang, Jingwen Li, Erning Chen, Tian Hong, Lulu Zhao, Meihong Du   +6 more
doaj   +1 more source

Effect of Trastuzumab–HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab

Frontiers in Chemistry, 2022
Asparagine deamidation and aspartic acid isomerization in the complementarity determining regions (CDRs) of monoclonal antibodies may alter their affinity to the target antigen.
Baubek Spanov, Victoria Aboagye, Oladapo Olaleye, Natalia Govorukhina, Nico C. van de Merbel, Nico C. van de Merbel, Rainer Bischoff   +6 more
doaj   +1 more source

Structural consensus among antibodies defines the antigen binding site. [PDF]

PLoS Computational Biology, 2012
The Complementarity Determining Regions (CDRs) of antibodies are assumed to account for the antigen recognition and binding and thus to contain also the antigen binding site.
Vered Kunik, Bjoern Peters, Yanay Ofran
doaj   +1 more source

The biological activity of soluble antigen-antibody complexes: II. Physical properties of soluble complexes having skin-irritating activity [PDF]

, 1959
Previous work by Germuth and McKinnon (1), Trapani et al. (2), and ourselves (3) has established the fact that soluble antigen-antibody complexes formed in excess antigen can, (a) induce symptoms similar to anaphylaxis, (b) cause contraction of isolated ...
Campbell, Dan H., Ishizaka, Kimishige, Ishizaka, Teruko   +2 more
core   +1 more source

Improved Diagnosis of Tuberculous Meningitis Using a Combination of Multiplex Antigens and Antibodies Testing Methods [PDF]

Journal of Clinical and Diagnostic Research, 2021
Introduction: Various serological assays exists, including Antigen and Antibody detection (IgM and IgG) for diagnosis of Tuberculous Meningitis (TBM) cases.
Amit Raju Nayak, Payal Rajendra Khulkhule, Vinita Rajendra Hutke, Arti Ramkumar Mishra, Nitin Harinarayan Chandak, Hatim F Daginawala, Lokendra R Singh, Rajpal Singh Kashya   +7 more
doaj   +1 more source