Results 81 to 90 of about 3,030,865 (307)

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Antigen-presentation efficacy of PLGA NPs-pulsed DCs.

, 2017
(A) Antigen-presentation efficacy of PLGA NPs-pulsed DCs to naive splenocytes. Splenocytes treated with medium alone served as negative control. The results are the mean stimulation index (S.I.)±SD from three independent experiments.
Evita Athanasiou (2265688), Costas Kiparissides (3691672), Katerina Kontonikola (3691657), Maritsa Margaroni (3691669), Evdokia Karagouni (2265694), Maria Agallou (2265691), Olga Kammona (3691660), Dimitra K. Toubanaki (3691666), Konstantina Karidi (3691663)   +8 more
core   +1 more source

Robust Control of a Brain-Persisting Parasite through MHC I Presentation by Infected Neurons

Cell Reports, 2019
Summary: Control of CNS pathogens by CD8 T cells is key to avoid fatal neuroinflammation. Yet, the modalities of MHC I presentation in the brain are poorly understood.
Anna Salvioni, Marcy Belloy, Aurore Lebourg, Emilie Bassot, Vincent Cantaloube-Ferrieu, Virginie Vasseur, Sophie Blanié, Roland S. Liblau, Elsa Suberbielle, Ellen A. Robey, Nicolas Blanchard   +10 more
doaj   +1 more source

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

How structural disorder and polyelectrolyte properties of prothymosin α enable its large interactome

FEBS Letters, EarlyView.
In this review, complexes of the fully disordered and highly negatively charged prothymosin α are collected, described, and discussed. Linked to its poly‐electrostatic character, prothymosin α engages a large interactome through multiple different binding modes ranging from fully disordered complexes to short linear motif (SLiM)‐driven interactions ...
Ketty C. Tamburrini, Freia S. Buus, Benjamin Schuler, Birthe B. Kragelund   +3 more
wiley   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Peptide-MHC-I from Endogenous Antigen Outnumber Those from Exogenous Antigen, Irrespective of APC Phenotype or Activation.

PLoS Pathogens, 2015
Naïve anti-viral CD8+ T cells (TCD8+) are activated by the presence of peptide-MHC Class I complexes (pMHC-I) on the surface of professional antigen presenting cells (pAPC).
Janet J Sei, Scott Haskett, Lauren W Kaminsky, Eugene Lin, Mary E Truckenmiller, Clifford J Bellone, R Mark Buller, Christopher C Norbury   +7 more
doaj   +1 more source

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source

Escaping high viral load exhaustion: CD8 cells with altered tetramer binding in chronic hepatitis B virus infection [PDF]

, 2002
Deletion, anergy, and a spectrum of functional impairments can affect virus-specific CD8 cells in chronic viral infections. Here we characterize a low frequency population of CD8 cells present in chronic hepatitis B virus (HBV) infection which survive in
Antonio Bertoletti, Brown, D., Dusheiko, G., Geoff Dusheiko, Williams, R., George J.M. Webster, Webster, G.J.M., David Brown, Mala K. Maini, Stephanie Reignat, King, A., Suranjith L. Seneviratne, Roger Williams, Bertoletti, A., Reignat, S., Maini, M.K., Graham S. Ogg, Abigail King, Ogg, G.S., Seneviratne, S.L.   +19 more
core   +1 more source