Results 101 to 110 of about 53,726 (303)

MetaboSense: An Integrated Quantum Dot‐Mediated Aptamer Assay and Modular Microfluidic Platform for Continuous In‐Line Monitoring of Lung Metabolism During Ex Vivo Perfusion

Advanced Science, EarlyView.
MetaboSense is a first‐of‐its‐kind microfluidic platform enabling continuous, in‐line monitoring of glucose and lactate during ex vivo lung perfusion. Combining a quantum dot–mediated aptamer assay with mixing, depletion, and optofluidic detection modules, it achieves 60–s temporal resolution and shows strong agreement with standard blood gas analyzers,
Sanjana Srikant, Pouyan Keshavarz Motamed, Thomas Borrillo, Lielle Ronen, Gabriel Siebiger, Guillermo Garza, Hesam Abouali, Md Fahim Al Fattah, Ines Jaber, Dayan Ban, Marcelo Cypel, Mingyao Liu, Shaf Keshavjee, Andrew T. Sage, Mahla Poudineh   +14 more
wiley   +1 more source

Controllable Cascade Aggregation Ion Programmed Nanomachines and Simple Isolation Enable Tumor‐Derived Small Extracellular Vesicles Detection for Enhanced Breast Cancer Assessment

Advanced Science, EarlyView.
This study developed a convenient, enzyme‐free detection platform based on controllable aggregation ion nanomachines. Its favorable anti‐interference capacity and high sensitivity meet the detection requirements for clinical samples processed via a simple filtration method within 10 min.
Zhihao Zhang, Zijing Liu, Xiangyue Meng, Pengjun Jiang, Jie Qu, Yu Jiang, Binxu Qiu, Jie Chen, Piaopiao Chen   +8 more
wiley   +1 more source

Interactions of Ligand, Aptamer, and Complementary Oligonucleotide: Studying Impacts of Na⁺ and Mg²⁺ Cations on Sensitive FRET-Based Detection of Aflatoxin B1

Molecules
The effects of magnesium and sodium on the interactions between aptamer, its specific ligand, and short complementary oligonucleotides (cDNAs) differing in affinity of their binding with the aptamer were studied.
Alexey V. Samokhvalov, Oksana G. Maksimenko, Sergei A. Eremin, Anatoly V. Zherdev, Boris B. Dzantiev   +4 more
doaj   +1 more source

Control of Aptamer Function Using Radiofrequency Magnetic Field

Journal of Nucleic Acids, 2011
Remote control of aptamer function has allowed us to control protein function in space and time. Here, we propose a novel control system for aptamer function by radiofrequency magnetic field- (RFMF-) induced local heating of a gold nanoparticle ...
Kenichi Taira, Koichi Abe, Takayuki Ishibasi, Katsuaki Sato, Kazunori Ikebukuro   +4 more
doaj   +1 more source

Supramolecular Degraders: An Emerging Paradigm in Targeted Protein Degradation

Advanced Science, EarlyView.
Dynamic supramolecular assembly reshapes targeted protein degradation by coordinating modular degrader construction, delivery, functional integration, and intracellular assembly or activation across proteasomal, endosomal–lysosomal, and autophagy–lysosomal pathways.
Kongjun Liu, Xue Yuan, Yan Zhou, Rui Yuan, Shaocheng Xie, Yong Chen, Peiyi Wang, Jianmei Gao, Qihai Gong   +8 more
wiley   +1 more source

Aptamer Generation and Bioapplication

Aptamers are bioaffinity molecules with many advantages, including in vitro generation, low cost, and a wide acceptance of chemical modification. Combined with transduction technologies, aptamers can be further utilized to develop aptasensors, which have

core   +1 more source

Myogenic Anti-Nucleolin Aptamer iSN04 Inhibits Proliferation and Promotes Differentiation of Vascular Smooth Muscle Cells [PDF]

De-differentiation and subsequent increased proliferation and inflammation of vascular smooth muscle cells (VSMCs) is one of the mechanisms of atherogenesis.
Shimosato, Takeshi, Miyoshi, Mana, Takaya, Tomohide, Tomohide Takaya, Takeshi Shimosato, Mana Miyoshi   +5 more
core   +1 more source

Rapid Exonuclease‐Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid‐Phase Ion Trap Coupled With Mass Spectrometry

Advanced Science, EarlyView.
A liquid‐phase ion trap (LPIT) coupled with ESI‐MS enables rapid, high‐fidelity de novo sequencing of long oligonucleotides in ∼30 min. By applying an electric field to dissociate metal adducts in solution, LPIT delivers clean spectra, superior mass accuracy, and full sequence coverage, surpassing MALDI‐TOF and LC‐MS.
Song Lu, Jie Hong, Xinyan Fu, Di Wang, Yunhua Gao, Wei Xu   +5 more
wiley   +1 more source

RNA Aptamer Binds Heparin-Binding Epidermal Growth Factor-Like Growth Factor with High Affinity and Specificity and Neutralizes Its Activity

International Journal of Gerontology, 2017
Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the EGF family and is involved in various diseases including cancers.
Masaki Yamato, Takashi Matsuzaki, Ryo Araki, Shota Tsuchida, Keiji Okuda, Hai Ying Fu, Shoji Sanada, Hiroshi Asanuma, Yoshihiro Asano, Masanori Asakura, Hiroomi Torii, Kentaro Noi, Hirotsugu Ogi, Ryo Iwamoto, Eisuke Mekada, Seiji Takashima, Masafumi Kitakaze, Yasushi Sakata, Tetsuo Minamino   +18 more
doaj   +1 more source

Aptamer-based fluorometric determination of Salmonella Typhimurium using Fe₃O₄ magnetic separation and CdTe quantum dots - Fig 5

, 2019
UV-vis absorption spectrum of MNPs@aptamer (curve a) and aptamer (curve b).
Junan Ren (3847069), Gang Liang (120372), An Li (415945), Ligang Pan (762190), Yan Man (6858176), Xinxin Jin (678016), Qingju Liu (2562268)   +6 more
core   +1 more source