Results 11 to 20 of about 48,450 (301)

DNA-Based Molecular Engineering of the Cell Membrane

Membranes, 2022
The cell membrane serves as a barrier and gatekeeper to regulate the cellular transportation of substances and information. It plays a significant role in protecting the cell from the extracellular environment, maintaining intracellular homeostasis, and ...
Xiaodong Li, Tiantian Wang, Yue Sun, Chang Li, Tianhuan Peng, Liping Qiu   +5 more
doaj   +1 more source

Computational design of single-stranded DNA hairpin aptamers immobilized on a biosensor substrate

Scientific Reports, 2021
Aptamer interactions with a surface of attachment are central to the design and performance of aptamer-based biosensors. We have developed a computational modeling approach to study different system designs—including different aptamer-attachment ends ...
Iman Jeddi, Leonor Saiz
doaj   +1 more source

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Frontiers in Bioengineering and Biotechnology, 2022
Various biosensors based on aptamers are currently the most popular rapid detection approaches, but the performance of these sensors is closely related to the affinity of aptamers.
Yadi Qin, Yanan Qin, Hayilati Bubiajiaer, Fengxia Chen, Jun Yao, Minwei Zhang   +5 more
doaj   +1 more source

Aptamers in analytics [PDF]

The Analyst, 2016
Nucleic acid aptamers are promising alternatives to antibodies in analytics.
Ilgu, Muslum, Nilsen-Hamilton, Marit
openaire   +3 more sources

Aptamers as therapeutics [PDF]

Nature Reviews Drug Discovery, 2010
Nucleic acid aptamers can be selected from pools of random-sequence oligonucleotides to bind a wide range of biomedically relevant proteins with affinities and specificities that are comparable to antibodies. Aptamers exhibit significant advantages relative to protein therapeutics in terms of size, synthetic accessibility and modification by medicinal ...
Keefe, Anthony D., Pai, Supriya, Ellington, Andrew   +2 more
openaire   +2 more sources

Enhancement of in vivo targeting properties of ErbB2 aptamer by chemical modification.

PLoS ONE, 2023
Aptamers have great potential for diagnostics and therapeutics due to high specificity to target molecules. However, studies have shown that aptamers are rapidly distributed and excreted from blood circulation due to nuclease degradation.
Jun Young Park, Ye Lim Cho, Ju Ri Chae, Jung Hwan Lee, Won Jun Kang   +4 more
doaj   +1 more source

Development of DNA Aptamers to Visualize Release of Mycobacterial Membrane-Derived Extracellular Vesicles in Infected Macrophages

Pharmaceuticals, 2021
Extracellular vesicles (EVs) have emerged into a novel vaccine platform, a biomarker and a nano-carrier for approved drugs. Their accurate detection and visualization are central to their utility in varied biomedical fields.
Soonjyoti Das, Sapna Jain, Mohd Ilyas, Anjali Anand, Saurabh Kumar, Nishant Sharma, Kuljit Singh, Rahul Mahlawat, Tarun Kumar Sharma, Krishnamohan Atmakuri   +9 more
doaj   +1 more source

Aptamers as Therapeutics [PDF]

Annual Review of Pharmacology and Toxicology, 2017
Aptamers are single-stranded nucleic acid molecules that bind to and inhibit proteins and are commonly produced by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers undergo extensive pharmacological revision, which alters affinity, specificity, and therapeutic half-life, tailoring each drug for a specific clinical need.
Shahid M, Nimjee, Rebekah R, White, Richard C, Becker, Bruce A, Sullenger   +3 more
openaire   +2 more sources

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Levisson, M., Smidt, H., Ruigrok, V.J.B., Mark Levisson, Dijkstra, B.W., Hekelaar, J., Dijkstra, Bauke W., Hauke Smidt, Ruigrok, Vincent J.B.,, van der Oost, John, Bauke W. Dijkstra, Smidt, Hauke,, Hekelaar, Johan, Dijkstra, Bauke W.,, Hekelaar, Johan,, Vincent J. B. Ruigrok, Levisson, Mark,, Oost, J., van der, Levisson, Mark, Smidt, Hauke, Johan Hekelaar, John Van der Oost, van der Oost, J., Oost, John van der,, Ruigrok, Vincent J. B.   +24 more
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Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy

, 2022
Expansion microscopy (ExM) is a newly developed technology in recent years that enables nanoscale imaging under conventional microscopes. Herein, we report an aptamer-based ExM imaging strategy.
Yue Sun, Jing Liu, Xiangjun Liu (1778443), Nan Zhang (46264), Jianhua Wang (159545), Weiwei Li, Jing Liu (38537), Dihua Shangguan (359783), Zhicheng Zhang, Yue Sun (197395), Zhicheng Zhang (490080), Yang Shu (528866), Jianhua Wang, Yang Shu, Tao Bing, Weiwei Li (364036), Dihua Shangguan, Tao Bing (407658), Xiangjun Liu, Nan Zhang   +19 more
core   +1 more source