Results 21 to 30 of about 53,726 (303)

DNA-Based Molecular Engineering of the Cell Membrane

Membranes, 2022
The cell membrane serves as a barrier and gatekeeper to regulate the cellular transportation of substances and information. It plays a significant role in protecting the cell from the extracellular environment, maintaining intracellular homeostasis, and ...
Xiaodong Li, Tiantian Wang, Yue Sun, Chang Li, Tianhuan Peng, Liping Qiu   +5 more
doaj   +1 more source

Aptamer Bioinformatics [PDF]

International Journal of Molecular Sciences, 2017
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization.
Andrew Kinghorn, Lewis Fraser, Shaolin Liang, Simon Shiu, Julian Tanner   +4 more
openaire   +2 more sources

Computational design of single-stranded DNA hairpin aptamers immobilized on a biosensor substrate

Scientific Reports, 2021
Aptamer interactions with a surface of attachment are central to the design and performance of aptamer-based biosensors. We have developed a computational modeling approach to study different system designs—including different aptamer-attachment ends ...
Iman Jeddi, Leonor Saiz
doaj   +1 more source

Development of DNA Aptamers to Visualize Release of Mycobacterial Membrane-Derived Extracellular Vesicles in Infected Macrophages

Pharmaceuticals, 2021
Extracellular vesicles (EVs) have emerged into a novel vaccine platform, a biomarker and a nano-carrier for approved drugs. Their accurate detection and visualization are central to their utility in varied biomedical fields.
Soonjyoti Das, Sapna Jain, Mohd Ilyas, Anjali Anand, Saurabh Kumar, Nishant Sharma, Kuljit Singh, Rahul Mahlawat, Tarun Kumar Sharma, Krishnamohan Atmakuri   +9 more
doaj   +1 more source

Aptamers as therapeutics [PDF]

Nature Reviews Drug Discovery, 2010
Nucleic acid aptamers can be selected from pools of random-sequence oligonucleotides to bind a wide range of biomedically relevant proteins with affinities and specificities that are comparable to antibodies. Aptamers exhibit significant advantages relative to protein therapeutics in terms of size, synthetic accessibility and modification by medicinal ...
Keefe, Anthony D., Pai, Supriya, Ellington, Andrew   +2 more
openaire   +2 more sources

Aptamers in analytics [PDF]

The Analyst, 2016
Nucleic acid aptamers are promising alternatives to antibodies in analytics.
Ilgu, Muslum, Nilsen-Hamilton, Marit
openaire   +3 more sources

Conformational dynamics of the tetracycline-binding aptamer [PDF]

, 2011
The conformational dynamics induced by ligand binding to the tetracycline-binding aptamer is monitored via stopped-flow fluorescence spectroscopy and time-correlated single photon counting experiments.
Weigand, Julia E., Josef Wachtveitl, Suess, Beatrix, Ute Förster, Trojanowski, Peter, Peter Trojanowski, Julia E. Weigand, Förster, Ute, Wachtveitl, Josef, Süß, Beatrix, Beatrix Suess   +10 more
core   +1 more source

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Frontiers in Bioengineering and Biotechnology, 2022
Various biosensors based on aptamers are currently the most popular rapid detection approaches, but the performance of these sensors is closely related to the affinity of aptamers.
Yadi Qin, Yanan Qin, Hayilati Bubiajiaer, Fengxia Chen, Jun Yao, Minwei Zhang   +5 more
doaj   +1 more source

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Levisson, M., Smidt, H., Ruigrok, V.J.B., Mark Levisson, Dijkstra, B.W., Hekelaar, J., Dijkstra, Bauke W., Hauke Smidt, Ruigrok, Vincent J.B.,, van der Oost, John, Bauke W. Dijkstra, Smidt, Hauke,, Hekelaar, Johan, Dijkstra, Bauke W.,, Hekelaar, Johan,, Vincent J. B. Ruigrok, Levisson, Mark,, Oost, J., van der, Levisson, Mark, Smidt, Hauke, Johan Hekelaar, John Van der Oost, van der Oost, J., Oost, John van der,, Ruigrok, Vincent J. B.   +24 more
core   +3 more sources

Dissecting the influence of Mg2+ on 3D architecture and ligand-binding of the guanine-sensing riboswitch aptamer domain [PDF]

, 2010
Long-range tertiary interactions determine the three-dimensional structure of a number of metabolite-binding riboswitch RNA elements and were found to be important for their regulatory function. For the guanine-sensing riboswitch of the Bacillus subtilis
Wöhnert, Jens, Buck, Janina, Schwalbe, Harald (Prof. Dr.), Noeske, Jonas   +3 more
core   +1 more source