Identification and characterization of DNA aptamers specific to VP2 protein of canine parvovirus
Applied Microbiology and Biotechnology, 2021 Canine parvovirus‐2 (CPV‐2) is ubiquitously distributed in dog population worldwide causing a severe and often fatal gastroenteritis. Owing to its highly contagious nature, rapid detection of CPV is crucial in effective control of the disease.
Mithilesh Singh +10 more
semanticscholar +1 more source
Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules [PDF]
, 2013 Carbon nanotube (CNT) decorated magnetic microspheres were fabricated to develop a multimodal platform that utilizes non-covalent molecular interactions of CNTs to magnetically separate biomolecules.
Kolkar Mohammed, Javed Hussain Niazi +1 more
core +1 more source
Site-Selective Ligand Selection by Mutational Profiling for Covalent RNA Targeting. [PDF]
Angew Chem Int Ed EnglUsing a mutational profiling approach an RNA‐targeted covalent probe was developed that is highly selective for the FMN riboswitch. The covalency was further supported by mass spectrometry experiments. The covalent probe selectively modifies the riboswitch at low micromolar concentrations within 10 min and remains functional when applied to an entire ...
Yesley P +3 more
europepmc +3 more sources
Insights into ligand binding to PreQ1 Riboswitch Aptamer from molecular dynamics simulations. [PDF]
PLoS ONE, 2014 Riboswitches play roles in transcriptional or translational regulation through specific ligand binding of their aptamer domains. Although a number of ligand-bound aptamer complex structures have been solved, it is important to know ligand-free ...
Zhou Gong +4 more
doaj +1 more source
The Validation and Amplification of Random DNA Libraries with Modified Nucleobases for Click-SELEX
Biology and Life Sciences Forum, 2022 Aptamers are nucleic acid ligands which exhibit specific binding to a desired target. Oligonucleotides are limited in their ability to form hydrophobic interactions.
Natalia Komarova +2 more
doaj +1 more source
Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity. [PDF]
, 2010 The ability to generate RNA aptamers for synthetic biology using in vitro selection depends on the informational complexity (IC) needed to specify functional structures that bind target ligands with desired affinities in physiological concentrations of ...
Carothers, James M +4 more
core +2 more sources
Next‐Generation Nucleic Acid Aptamers with Two‐Base‐Modified Nucleotides Have Improved Binding Affinity and Potency [PDF]
ChemBioChem, 2017 Twice as apt: Nucleic acid aptamers with high binding affinity, specificity, epitope coverage and nuclease resistance were developed by using libraries containing oligonucleotides in which two bases in the pyrimidine nucleotide had been modified.
Hadi, AlShamaileh, Rakesh N, Veedu
openaire +2 more sources
Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries
Journal of Nucleic Acids, 2012 Specific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research.
Yuuya Kasahara, Masayasu Kuwahara
doaj +1 more source
Incorporation of aptamers in the terminal loop of shRNAs yields an effective and novel combinatorial targeting strategy. [PDF]
, 2017 Gene therapy by engineering patient's own blood cells to confer HIV resistance can potentially lead to a functional cure for AIDS. Toward this goal, we have previously developed an anti-HIV lentivirus vector that deploys a combination of shRNA, ribozyme ...
Castanotto, Daniela +4 more
core +1 more source
Angewandte Chemie, 2020 RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence‐based strategies reveal information on structure and dynamics of RNA aptamers.
C. Steinmetzger +2 more
semanticscholar +1 more source