Results 51 to 60 of about 13,012 (166)

Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

Frontiers in Chemistry, 2015
Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target.
Emmanuelle eFiore, Eric eDausse, Hervé eDubouchaud, Eric ePeyrin, Corinne eRavelet   +4 more
doaj   +1 more source

Efficient Reverse Transcription Using Locked Nucleic Acid Nucleotides towards the Evolution of Nuclease Resistant RNA Aptamers

PLoS ONE, 2012
Modified nucleotides are increasingly being utilized in the de novo selection of aptamers for enhancing their drug-like character and abolishing the need for time consuming trial-and-error based post-selection modifications. Locked nucleic acid (LNA) is one of the most prominent and successful nucleic acid analogues because of its remarkable properties,
Crouzier, Lucile; Dubois, Camille; Edwards, Stacey L.; Lauridsen, Lasse H.; Wengel, Jesper; Veedu, Rakesh, N.   +7 more
openaire   +9 more sources

Supramolecular Aptamers on Graphene Oxide for Efficient Inhibition of Thrombin Activity

Frontiers in Chemistry, 2019
Graphene oxide (GO), a two-dimensional material with a high aspect ratio and polar functional groups, can physically adsorb single-strand DNA through different types of interactions, such as hydrogen bonding and π-π stacking, making it an attractive ...
Ting-Xuan Lin, Pei-Xin Lai, Ju-Yi Mao, Ju-Yi Mao, Ju-Yi Mao, Han-Wei Chu, Binesh Unnikrishnan, Anisha Anand, Chih-Ching Huang, Chih-Ching Huang, Chih-Ching Huang   +10 more
doaj   +1 more source

Identification of Amino Acids and Nucleotides Required for Interaction Between BAFF‐R and its RNA Aptamer

The FASEB Journal, 2015
Current cancer therapies such as surgery, radiation, and chemotherapy can leave residual tumor cells and have off‐target effects. Aptamers are used in a type of targeted cancer therapy that more precisely identify and attack cancer cells, while leaving unaffected cells unharmed. In B‐cell malignancies, such as non‐Hodgkins lymphoma,
Monica Lares, John Rossi
openaire   +1 more source

Energy-based method for designing aptamers to target phosphatidylcholine

Medicine in Drug Discovery
Diverse technologies are used to design nucleic acid aptamers to achieve targeted therapeutics, engineer diagnostic tools to detect specific biomolecules associated with disease-oriented signaling pathways, and develop drug delivery systems.
Fatima Alharbi, Hamed Alsulami, Suliman AlOmar, Md. Ashrafuzzaman   +3 more
doaj   +1 more source

The tyranny of adenosine recognition among RNA aptamers to coenzyme A

BMC Evolutionary Biology, 2003
Background Understanding the diversity of interactions between RNA aptamers and nucleotide cofactors promises both to facilitate the design of new RNA enzymes that utilize these cofactors and to constrain models of RNA World evolution.
Frank Joseph, Saran Dayal, Burke Donald H   +2 more
doaj   +1 more source

Synthesis and Enzymatic Incorporation of Modified Deoxyuridine Triphosphates

Molecules, 2015
To expand the chemical functionality of DNAzymes and aptamers, several new modified deoxyuridine triphosphates have been synthesized. An important precursor that enables this aim is 5-aminomethyl dUTP, whereby the pendent amine serves as a handle for ...
Erkai Liu, Curtis H. Lam, David M. Perrin   +2 more
doaj   +1 more source

Effects of 5-Methyl-2′-Deoxycytidine in G-Quadruplex Forming Aptamers d(G₃C)₄ and d[GCG₂(CG₃)₃C]: Investigating the Key Role of the Loops

Biomolecules
T40214 (STAT) and its recently investigated analogue STATB are G-quadruplex (G4) forming aptamers characterized by an unusually high percentage of C. The therapeutic potential of T40214 relies on its ability to inhibit the signalling pathway of STAT3, a ...
Veronica Esposito, Daniela Benigno, Carla Aliberti, Camilla Esposito, Elisabetta Panza, Antonella Virgilio, Aldo Galeone   +6 more
doaj   +1 more source

DNA Sensors with Diamond as a Promising Alternative Transducer Material

Sensors, 2009
Bio-electronics is a scientific field coupling the achievements in biology with electronics to obtain higher sensitivity, specificity and speed. Biosensors have played a pivotal role, and many have become established in the clinical and scientific world.
Luc Michiels, Patrick Wagner, Veronique Vermeeren, Sylvia Wenmackers   +3 more
doaj   +1 more source

AXL receptor tyrosine kinase as a promising anti-cancer approach: functions, molecular mechanisms and clinical applications

Molecular Cancer, 2019
Molecular targeted therapy for cancer has been a research hotspot for decades. AXL is a member of the TAM family with the high-affinity ligand growth arrest-specific protein 6 (GAS6). The Gas6/AXL signalling pathway is associated with tumour cell growth,
Chenjing Zhu, Yuquan Wei, Xiawei Wei
doaj   +1 more source