Results 31 to 40 of about 13,012 (166)
Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
Scientific Reports, 2021 Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts.
Tahmineh Jalali +3 more
doaj +1 more source
Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides [PDF]
Biosensors and Bioelectronics, 2015 We previously reported a fluorescence anisotropy (FA) approach for small molecules using tetramethylrhodamine (TMR) labeled aptamer. It relies on target-binding induced change of intramolecular interaction between TMR and guanine (G) base. TMR-labeling sites are crucial for this approach. Only terminal ends and thymine (T) bases could be tested for TMR
Qiang, Zhao, Qin, Lv, Hailin, Wang
openaire +2 more sources
Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation
Molecules, 2009 The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA ...
Meltem Avci-Adali +4 more
doaj +1 more source
Pharmaceuticals, 2022 Von Willebrand Factor (VWF) plays a critical role in thrombus formation, stabilization, and propagation. Previous studies have demonstrated that targeted inhibition of VWF induces thrombolysis when administered in vivo in animal models of ischemic stroke.
Susan M. Shea +7 more
doaj +1 more source
Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides [PDF]
Chemical Communications, 2012 Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
Dubois, C. +4 more
openaire +6 more sources
Life, 2020 In the present in vitro selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon Aeropyrum pernix.
Senri Ohmori +6 more
doaj +1 more source
Agreement of aptamer proteomics with standard methods for measuring venous thrombosis biomarkers
Research and Practice in Thrombosis and Haemostasis, 2021 Background Venous thromboembolism (VTE) is a complex disease with an incidence rate of about 1 in 1000 per year. Despite the availability of validated biomarkers for VTE, unprovoked events account for 50% of first events.
Tariq Faquih +5 more
doaj +1 more source
Biochemistry, 2020 The ability to change binding affinity in a controlled fashion is a key step in the rational design of biomolecules in general and functional nucleic acids in particular. Here, we use dangling nucleotides to alter the binding affinity of structure-switching aptamers.
Sladjana Slavkovic +2 more
openaire +3 more sources
The Diagnostic and Therapeutic Potential of Oligonucleotide Aptamers in Alzheimer’s Disease
CellsAlzheimer’s disease (AD) is the neurodegenerative condition with the outmost future challenges, with timely diagnosis and treatment being the most urgent.
Georgios Katsipis +3 more
doaj +1 more source
Probing Essential Nucleobase Functional Groups in Aptamers and Deoxyribozymes by Nucleotide Analogue Interference Mapping of DNA [PDF]
Journal of the American Chemical Society, 2011 Nucleotide analogue interference mapping of DNA (dNAIM) is here introduced as a new nonenzymatic interference-based approach that enables high-throughput identification of essential nucleobase functional groups in DNA aptamers and in the catalytic core of deoxyribozymes.
Wachowius, F., Höbartner, C.
openaire +3 more sources