Results 31 to 40 of about 13,014 (201)
Design and clinical developments of aptamer-drug conjugates for targeted cancer therapy
Biomaterials Research, 2021 Background Aptamer has been called “chemical antibody” which displays the specific affinity to target molecules compared to that of antibodies and possesses several therapeutic advantages over antibodies in terms of size, accessibility to synthesis, and ...
Do-Hun Kim +3 more
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Application of Aptamers in Virus Detection and Antiviral Therapy
Frontiers in Microbiology, 2019 Viral infections can cause serious diseases for humans and animals. Accurate and early detection of viruses is often crucial for clinical diagnosis and therapy. Aptamers are mostly single-stranded nucleotide sequences that are artificially synthesized by
Xinran Zou +7 more
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ChemBioChem, 2023 AbstractChemical modification of aptamers is an important step to improve their performance and stability in biological media. This can be performed either during their identification (mod‐SELEX) or after the in vitro selection process (post‐SELEX).
Germain Niogret +12 more
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Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides [PDF]
Biosensors and Bioelectronics, 2015 We previously reported a fluorescence anisotropy (FA) approach for small molecules using tetramethylrhodamine (TMR) labeled aptamer. It relies on target-binding induced change of intramolecular interaction between TMR and guanine (G) base. TMR-labeling sites are crucial for this approach. Only terminal ends and thymine (T) bases could be tested for TMR
Qiang, Zhao, Qin, Lv, Hailin, Wang
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Trends in Medical Sciences, 2021 : Targeted cancer therapy is developing rapidly according to the fact that it has been demonstrated that this type of therapy can reduce various side effects and adverse events of the commonly available cancer treatment approaches such as chemotherapy and radiotherapy.
Pouya Safarzadeh Kozani +2 more
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Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
Scientific Reports, 2021 Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts.
Tahmineh Jalali +3 more
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Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides [PDF]
Chemical Communications, 2012 Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
Dubois, C. +4 more
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Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation
Molecules, 2009 The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA ...
Meltem Avci-Adali +4 more
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Pharmaceuticals, 2022 Von Willebrand Factor (VWF) plays a critical role in thrombus formation, stabilization, and propagation. Previous studies have demonstrated that targeted inhibition of VWF induces thrombolysis when administered in vivo in animal models of ischemic stroke.
Susan M. Shea +7 more
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Biochemistry, 2020 The ability to change binding affinity in a controlled fashion is a key step in the rational design of biomolecules in general and functional nucleic acids in particular. Here, we use dangling nucleotides to alter the binding affinity of structure-switching aptamers.
Sladjana Slavkovic +2 more
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