Prediction of aptamer-target interacting pairs with pseudo-amino acid composition. [PDF]
PLoS ONE, 2014 Aptamers are oligonucleic acid or peptide molecules that bind to specific target molecules. As a novel and powerful class of ligands, aptamers are thought to have excellent potential for applications in the fields of biosensing, diagnostics and ...
Bi-Qing Li +5 more
doaj +1 more source
Structure–Activity Relationship Study of a Potent α‐Thrombin Binding Aptamer Incorporating Hexitol Nucleotides [PDF]
Chemistry – A European Journal, 2020 AbstractThe replacement of one or more nucleotide residues in the potent α‐thrombin‐binding aptamer NU172 with hexitol‐based nucleotides has been devised to study the effect of these substitutions on the physicochemical and functional properties of the anticoagulant agent.
De Fenza, Maria +8 more
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Construction of a Bivalent Thrombin Binding Aptamer and Its Antidote with Improved Properties
Molecules, 2017 Aptamers are short synthetic DNA or RNA oligonucleotides that adopt secondary and tertiary conformations based on Watson–Crick base-pairing interactions and can be used to target a range of different molecules.
Quintin W. Hughes +4 more
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Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]
PLoS ONE, 2010 BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann +4 more
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Enzymatic Synthesis of Vancomycin-Modified DNA
Molecules, 2022 Many potent antibiotics fail to treat bacterial infections due to emergence of drug-resistant strains. This surge of antimicrobial resistance (AMR) calls in for the development of alternative strategies and methods for the development of drugs with ...
Chiara Figazzolo +4 more
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Identification of Allosteric Nucleotide Sites of Tetramethylrhodamine-Labeled Aptamer for Noncompetitive Aptamer-Based Fluorescence Anisotropy Detection of a Small Molecule, Ochratoxin A [PDF]
Analytical Chemistry, 2013 Aptamer-based fluorescence anisotropy (FA) assay combines the advantages of affinity aptamers in good stability, easy generation, and facile labeling and the benefits of FA in homogeneous analysis, such as robustness, simplicity, and high reproducibility.
Qiang, Zhao, Qin, Lv, Hailin, Wang
openaire +2 more sources
Mature miRNAs form secondary structure, which suggests their function beyond RISC. [PDF]
PLoS ONE, 2014 The generally accepted model of the miRNA-guided RNA down-regulation suggests that mature miRNA targets mRNA in a nucleotide sequence-specific manner.
Agnieszka Belter +6 more
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Molecular Therapy: Nucleic Acids, 2021 Post-SELEX modification of DNA aptamers is an established strategy to improve their affinity or inhibitory characteristics. In this study, we examined the possibility of increasing the recognition interface between the thrombin-binding aptamer HD1 (TBA ...
Igor Smirnov +4 more
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PLoS ONE, 2021 Oligonucleotide-based aptamers, which have a three-dimensional structure with a single-stranded fragment, feature various characteristics with respect to size, toxicity, and permeability.
Gwangho Lee +3 more
doaj +1 more source
DNA Aptamers Block the Receptor Binding Domain at the Spike Protein of SARS-CoV-2
Frontiers in Molecular Biosciences, 2021 DNA aptamers are versatile molecular species obtained by the folding of short single-stranded nucleotide sequences, with highly specific recognition capabilities against proteins.
Fabrizio Cleri +3 more
doaj +1 more source