Results 11 to 20 of about 13,012 (166)

Analysis of interaction between RNA aptamer and protein using nucleotide analogs [PDF]

Nucleic Acids Symposium Series, 2000
Non-structural protein 3 (NS3) derived from Hepatitis C virus (HCV) is essential for viral proliferation and has two functional domains; trypsin-like serine protease and helicase. Recently we obtained three types of RNA aptamers (G9-I, -II and -III) bound to NS3 protease domain (delta NS3) by in vitro selection and confirmed their strong inhibition for
S, Sekiya, K, Fukuda, J, Hwang, N, Kakiuchi, K, Taira, I, Kusakabe, S, Nishikawa   +6 more
openaire   +2 more sources

Thrombin binding aptamer G-quadruplex stabilized by pyrene-modified nucleotides

Nucleic Acids Research, 2020
AbstractGuanine-rich regions of the human genome can adopt non-canonical secondary structures. Their role in regulating gene expression has turned them into promising targets for therapeutic intervention. Ligands based on polyaromatic moieties are especially suitable for targeting G-quadruplexes utilizing their size complementarity to interact with the
Janez Plavec, Naoki Sugimoto, Shuntaro Takahashi, Hisae Tateishi-Karimata, Peter Podbevšek, Matic Kovačič   +5 more
openaire   +2 more sources

Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

Heliyon, 2023
The emergence of the SARS-CoV-2 virus, an unknown strain of coronavirus, has resulted in severe acute respiratory syndrome with high mortality rates worldwide.
Zahra Sadat Moshref, Tahmineh Jalali, Razieh Rezaei Adriani, Elahe Soltati, Seyed Latif Mousavi Gargari   +4 more
doaj   +1 more source

Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases

ChemBioChem, 2020
AbstractOne of the pivotal steps in aptamer selection is the amplification of target‐specific oligonucleotides by thermophilic DNA polymerases; it can be a challenging task if nucleic acids possessing modified nucleotides are to be amplified. Hence, the identification of compatible DNA polymerase and modified nucleotide pairs is necessary for effective
Krisztina Percze, Tamás Mészáros
openaire   +2 more sources

Kinetic Exclusion Assay of Biomolecules by Aptamer Capture

Sensors, 2020
DNA aptamers are short nucleotide oligomers selected to bind a target ligand with affinity and specificity rivaling that of antibodies. These remarkable features recommend aptamers as candidates for analytical and therapeutic applications that ...
Mark H. Smith, Daniel Fologea
doaj   +1 more source

Prediction of aptamer-target interacting pairs with pseudo-amino acid composition. [PDF]

PLoS ONE, 2014
Aptamers are oligonucleic acid or peptide molecules that bind to specific target molecules. As a novel and powerful class of ligands, aptamers are thought to have excellent potential for applications in the fields of biosensing, diagnostics and ...
Bi-Qing Li, Yu-Chao Zhang, Guo-Hua Huang, Wei-Ren Cui, Ning Zhang, Yu-Dong Cai   +5 more
doaj   +1 more source

Towards the selection of phosphorothioate aptamers [PDF]

European Journal of Biochemistry, 2000
The high affinity of a given nucleic acid for a protein ligand can be used to isolate specific inhibitors of enzymes involved in pathological situations. The latter property is the basis of the SELEX (systematic evolution of ligands by exponential enrichment) technique.
M L, Andreola, C, Calmels, J, Michel, J J, Toulmé, S, Litvak   +4 more
openaire   +2 more sources

Construction of a Bivalent Thrombin Binding Aptamer and Its Antidote with Improved Properties

Molecules, 2017
Aptamers are short synthetic DNA or RNA oligonucleotides that adopt secondary and tertiary conformations based on Watson–Crick base-pairing interactions and can be used to target a range of different molecules.
Quintin W. Hughes, Bao T. Le, Grace Gilmore, Ross I. Baker, Rakesh N. Veedu   +4 more
doaj   +1 more source

Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]

PLoS ONE, 2010
BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann, Tanja Gesell, Doris Chen, Christina Lorenz, Renée Schroeder   +4 more
doaj   +1 more source

Enzymatic Synthesis of Vancomycin-Modified DNA

Molecules, 2022
Many potent antibiotics fail to treat bacterial infections due to emergence of drug-resistant strains. This surge of antimicrobial resistance (AMR) calls in for the development of alternative strategies and methods for the development of drugs with ...
Chiara Figazzolo, Frédéric Bonhomme, Saidbakhrom Saidjalolov, Mélanie Ethève-Quelquejeu, Marcel Hollenstein   +4 more
doaj   +1 more source