Results 41 to 50 of about 13,012 (166)

Insights into ligand binding to PreQ1 Riboswitch Aptamer from molecular dynamics simulations. [PDF]

PLoS ONE, 2014
Riboswitches play roles in transcriptional or translational regulation through specific ligand binding of their aptamer domains. Although a number of ligand-bound aptamer complex structures have been solved, it is important to know ligand-free ...
Zhou Gong, Yunjie Zhao, Changjun Chen, Yong Duan, Yi Xiao   +4 more
doaj   +1 more source

The Validation and Amplification of Random DNA Libraries with Modified Nucleobases for Click-SELEX

Biology and Life Sciences Forum, 2022
Aptamers are nucleic acid ligands which exhibit specific binding to a desired target. Oligonucleotides are limited in their ability to form hydrophobic interactions.
Natalia Komarova, Olga Panova, Aleksey Titov   +2 more
doaj   +1 more source

Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries

Journal of Nucleic Acids, 2012
Specific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research.
Yuuya Kasahara, Masayasu Kuwahara
doaj   +1 more source

Protein DEK and DTA Aptamers: Insight Into the Interaction Mechanisms and the Computational Aptamer Design

Frontiers in Molecular Biosciences, 2022
By blocking the DEK protein, DEK-targeted aptamers (DTAs) can reduce the formation of neutrophil extracellular traps (NETs) to reveal a strong anti-inflammatory efficacy in rheumatoid arthritis.
Lijun Dai, Jiangnan Zhang, Xiaonan Wang, Xiaoyue Yang, Feng Pan, Longhua Yang, Yongxing Zhao, Yongxing Zhao   +7 more
doaj   +1 more source

Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection

Communications Chemistry
Decoration of aptamers with chemical modifications at the level of nucleobases grants access to alternative binding modes, which often result in improved binding properties.
Greta Charlotte Dahm, Usman Akhtar, Alix Bouvier-Müller, Laura Lim, Fabienne Levi-Acobas, Pierre Nicolas Bizat, Germain Niogret, Julian A. Tanner, Frédéric Ducongé, Marcel Hollenstein   +9 more
doaj   +1 more source

Novel Strategies to Optimize the Amplification of Single-Stranded DNA

Frontiers in Bioengineering and Biotechnology, 2020
The generation of single stranded DNA plays a key role in in vitro selection of DNA aptamers and in other molecular techniques such as DNA sequencing and microarrays.
Atef Nehdi, Atef Nehdi, Atef Nehdi, Nosaibah Samman, Nosaibah Samman, Vanessa Aguilar-Sánchez, Azer Farah, Emre Yurdusev, Mohamed Boudjelal, Mohamed Boudjelal, Jonathan Perreault   +10 more
doaj   +1 more source

Aptamer-based multiplexed proteomic technology for biomarker discovery. [PDF]

PLoS ONE, 2010
The interrogation of proteomes ("proteomics") in a highly multiplexed and efficient manner remains a coveted and challenging goal in biology and medicine.We present a new aptamer-based proteomic technology for biomarker discovery capable of ...
Larry Gold, Deborah Ayers, Jennifer Bertino, Christopher Bock, Ashley Bock, Edward N Brody, Jeff Carter, Andrew B Dalby, Bruce E Eaton, Tim Fitzwater, Dylan Flather, Ashley Forbes, Trudi Foreman, Cate Fowler, Bharat Gawande, Meredith Goss, Magda Gunn, Shashi Gupta, Dennis Halladay, Jim Heil, Joe Heilig, Brian Hicke, Gregory Husar, Nebojsa Janjic, Thale Jarvis, Susan Jennings, Evaldas Katilius, Tracy R Keeney, Nancy Kim, Tad H Koch, Stephan Kraemer, Luke Kroiss, Ngan Le, Daniel Levine, Wes Lindsey, Bridget Lollo, Wes Mayfield, Mike Mehan, Robert Mehler, Sally K Nelson, Michele Nelson, Dan Nieuwlandt, Malti Nikrad, Urs Ochsner, Rachel M Ostroff, Matt Otis, Thomas Parker, Steve Pietrasiewicz, Daniel I Resnicow, John Rohloff, Glenn Sanders, Sarah Sattin, Daniel Schneider, Britta Singer, Martin Stanton, Alana Sterkel, Alex Stewart, Suzanne Stratford, Jonathan D Vaught, Mike Vrkljan, Jeffrey J Walker, Mike Watrobka, Sheela Waugh, Allison Weiss, Sheri K Wilcox, Alexey Wolfson, Steven K Wolk, Chi Zhang, Dom Zichi   +68 more
doaj   +1 more source

Next‐Generation Nucleic Acid Aptamers with Two‐Base‐Modified Nucleotides Have Improved Binding Affinity and Potency [PDF]

ChemBioChem, 2017
Twice as apt: Nucleic acid aptamers with high binding affinity, specificity, epitope coverage and nuclease resistance were developed by using libraries containing oligonucleotides in which two bases in the pyrimidine nucleotide had been modified.
Hadi, AlShamaileh, Rakesh N, Veedu
openaire   +2 more sources

The cotranscriptional folding landscape for two cyclic di-nucleotide-sensing riboswitches with highly homologous aptamer domains acting either as ON- or OFF-switches

Nucleic Acids Research, 2022
Abstract Riboswitches are gene regulatory elements located in untranslated mRNA regions. They bind inducer molecules with high affinity and specificity. Cyclic-di-nucleotide-sensing riboswitches are major regulators of genes for the environment, membranes and motility (GEMM) of bacteria.
Tom Landgraf, Albrecht Eduard Völklein, Boris Fürtig, Harald Schwalbe   +3 more
openaire   +3 more sources

Aptamers: precision tools for diagnosing and treating infectious diseases

Frontiers in Cellular and Infection Microbiology
Infectious diseases represent a significant global health challenge, with bacteria, fungi, viruses, and parasitic protozoa being significant causative agents.
Swathi Sujith, Rajalakshmi Naresh, B. U. Srivisanth, Anusree Sajeevan, Shobana Rajaramon, Helma David, Adline Princy Solomon   +6 more
doaj   +1 more source