Results 41 to 50 of about 13,014 (201)

RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution

Life, 2020
In the present in vitro selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon Aeropyrum pernix.
Senri Ohmori, Marina Wani, Saki Kitabatake, Yuka Nakatsugawa, Tadashi Ando, Takuya Umehara, Koji Tamura   +6 more
doaj   +1 more source

Agreement of aptamer proteomics with standard methods for measuring venous thrombosis biomarkers

Research and Practice in Thrombosis and Haemostasis, 2021
Background Venous thromboembolism (VTE) is a complex disease with an incidence rate of about 1 in 1000 per year. Despite the availability of validated biomarkers for VTE, unprovoked events account for 50% of first events.
Tariq Faquih, Dennis O. Mook‐Kanamori, Frits R. Rosendaal, Trevor Baglin, Ko Willems van Dijk, Astrid vanHylckama Vlieg   +5 more
doaj   +1 more source

The Diagnostic and Therapeutic Potential of Oligonucleotide Aptamers in Alzheimer’s Disease

Cells
Alzheimer’s disease (AD) is the neurodegenerative condition with the outmost future challenges, with timely diagnosis and treatment being the most urgent.
Georgios Katsipis, Eleni E. Tzekaki, Sophia Iasonidou, Anastasia A. Pantazaki   +3 more
doaj   +1 more source

Probing Essential Nucleobase Functional Groups in Aptamers and Deoxyribozymes by Nucleotide Analogue Interference Mapping of DNA [PDF]

Journal of the American Chemical Society, 2011
Nucleotide analogue interference mapping of DNA (dNAIM) is here introduced as a new nonenzymatic interference-based approach that enables high-throughput identification of essential nucleobase functional groups in DNA aptamers and in the catalytic core of deoxyribozymes.
Wachowius, F., Höbartner, C.
openaire   +3 more sources

Toward Multi(radio)metalated DNA: Enzymatic Polymerization of Metal‐Chelate‐Modified Deoxyribonucleoside Triphosphates

Angewandte Chemie, EarlyView.
This work presents the first enzymatic polymerization of defined metal‐chelator‐modified nucleotides that leverage polymerase fidelity for uniform (radio)metal loading with successful incorporation of five different metals: Ga, In, Tb, Lu, and Y.
Antonio A. W. L. Wong, François Bénard, David M. Perrin   +2 more
wiley   +2 more sources

Insights into ligand binding to PreQ1 Riboswitch Aptamer from molecular dynamics simulations. [PDF]

PLoS ONE, 2014
Riboswitches play roles in transcriptional or translational regulation through specific ligand binding of their aptamer domains. Although a number of ligand-bound aptamer complex structures have been solved, it is important to know ligand-free ...
Zhou Gong, Yunjie Zhao, Changjun Chen, Yong Duan, Yi Xiao   +4 more
doaj   +1 more source

The Validation and Amplification of Random DNA Libraries with Modified Nucleobases for Click-SELEX

Biology and Life Sciences Forum, 2022
Aptamers are nucleic acid ligands which exhibit specific binding to a desired target. Oligonucleotides are limited in their ability to form hydrophobic interactions.
Natalia Komarova, Olga Panova, Aleksey Titov   +2 more
doaj   +1 more source

AND‐Logic‐Gated Aptamer Switch for Precise Targeting and Regulation of RNA G‐Quadruplexes

Angewandte Chemie, EarlyView.
An AND‐logic‐gated aptamer switch enables tumor‐selective activation of RNA G‐quadruplex (rG4)‐targeting function. By combining cell‐surface recognition and intracellular redox sensing, the system achieves precise regulation of disease‐relevant RNA structures exclusively in tumor cells, with minimal off‐target effects in normal cells, offering a ...
Dan Wang, Ying Feng, Chun Kit Kwok
wiley   +2 more sources

Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries

Journal of Nucleic Acids, 2012
Specific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research.
Yuuya Kasahara, Masayasu Kuwahara
doaj   +1 more source

A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease

Angewandte Chemie, EarlyView.
A simple, rapid, and portable gold‐coated filter tip‐based assay (GFTA) is developed for bacterial detection. The GFTA leverages cleavable functional nucleic acid probes as molecular recognition elements to target RNase H2, a highly conserved bacterial endoribonuclease, enabling sensitive and specific colorimetric detection of bacterial pathogens ...
Jiuxing Li, Rudi Liu, Wenqing Zhang, Bruno J. Salena, Yingfu Li   +4 more
wiley   +2 more sources