Results 61 to 70 of about 13,014 (201)

Versatile DNA‐Functionalized Biohybrid Hydrogel Platforms for Electrothermally Activated On‐Demand Payload Release

Advanced Science, EarlyView.
A DNA‐functionalized biohybrid hydrogel is developed by integrating Cu‐mediated nucleic acid microflowers into a thermoresponsive matrix, enabling electrothermally triggered, on‐demand payload release. The platform offers spatiotemporally controllable therapeutic delivery with promising applications in precision oncology, neural regulation, and chronic
Mengqiu Sun, Zhenshuai Tang, Jia Zhang, Rui Song, Wei Luo, Juan Huang, Jincheng Zeng, Zhen He, Hao Zhang   +8 more
wiley   +1 more source

Next‐Generation Nucleic Acid Aptamers with Two‐Base‐Modified Nucleotides Have Improved Binding Affinity and Potency [PDF]

ChemBioChem, 2017
Twice as apt: Nucleic acid aptamers with high binding affinity, specificity, epitope coverage and nuclease resistance were developed by using libraries containing oligonucleotides in which two bases in the pyrimidine nucleotide had been modified.
Hadi, AlShamaileh, Rakesh N, Veedu
openaire   +2 more sources

The cotranscriptional folding landscape for two cyclic di-nucleotide-sensing riboswitches with highly homologous aptamer domains acting either as ON- or OFF-switches

Nucleic Acids Research, 2022
Abstract Riboswitches are gene regulatory elements located in untranslated mRNA regions. They bind inducer molecules with high affinity and specificity. Cyclic-di-nucleotide-sensing riboswitches are major regulators of genes for the environment, membranes and motility (GEMM) of bacteria.
Tom Landgraf, Albrecht Eduard Völklein, Boris Fürtig, Harald Schwalbe   +3 more
openaire   +3 more sources

A Solution for Exosome‐Based Analysis: Surface‐Enhanced Raman Spectroscopy and Artificial Intelligence

Advanced Intelligent Discovery, EarlyView.
Exosomes are emerging as powerful biomarkers for disease diagnosis and monitoring. This review highlights the integration of surface‐enhanced Raman spectroscopy with artificial intelligence to enhance molecular fingerprinting of exosomes. Machine learning and deep learning techniques improve spectral interpretation, enabling accurate classification of ...
Munevver Akdeniz, Zakarya Al‐Shaebi, Omer Aydin   +2 more
wiley   +1 more source

Aptamers: precision tools for diagnosing and treating infectious diseases

Frontiers in Cellular and Infection Microbiology
Infectious diseases represent a significant global health challenge, with bacteria, fungi, viruses, and parasitic protozoa being significant causative agents.
Swathi Sujith, Rajalakshmi Naresh, B. U. Srivisanth, Anusree Sajeevan, Shobana Rajaramon, Helma David, Adline Princy Solomon   +6 more
doaj   +1 more source

A Universal Nucleoside‐to‐Nucleoside‐5′‐Triphosphate Enzyme Cascade Driven by Polyphosphate

Angewandte Chemie International Edition, EarlyView.
Nucleoside‐5′‐triphosphates (NTPs) are essential components for the generation of nucleic acids, yet their chemical synthesis is hindered by the low yield of nucleoside phosphorylation. Here, we present a universal, two‐enzyme nucleoside‐to‐NTP cascade that is driven by polyphosphate.
Jonathan P. Suess, Nicolas V. Cornelissen   +1 more
wiley   +1 more source

Dual‐Channel Interdigitated Aptamer‐Based Sensors for Rapid Small‐Molecule Detection in Biofluids

Angewandte Chemie International Edition, EarlyView.
An electrochemical aptamer sensing chip employs dual interdigitated electrodes and selective self‐assembled monolayer removal to convert small‐molecule recognition into robust “signal‐off/on” responses. This dual‐channel signal conversion minimizes background noise and accelerates probe diffusion, enabling rapid (as fast as 5–30 min), low‐volume ...
Senyao Wang, Ali Elmorsy, Defne Tüzün, Lilly Schmidt, Sebastian Freko, Lukas Hiendlmeier, Chen Wang, Alonso Ingar Romero, George Al Boustani, Hu Peng, Berna Özkale, Nako Nakatsuka, Bernhard Wolfrum   +12 more
wiley   +1 more source

Decoding RNA regulation: Challenges and opportunities for RNA‐based therapies in Europe

British Journal of Clinical Pharmacology, EarlyView.
Abstract RNA‐based medicinal products represent a promising frontier in personalised medicine, offering sequence‐specific disease targeting at various molecular levels, yet their clinical translation in the European Union (EU) may be hindered by regulatory uncertainty around definitions and evidence requirements; this study therefore aims to identify ...
Olivia C. Lewis, Christine Leopold, Joyce M. Hoek, Raymond M. Schiffelers, Marie L. De Bruin   +4 more
wiley   +1 more source

Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

Frontiers in Chemistry, 2015
Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target.
Emmanuelle eFiore, Eric eDausse, Hervé eDubouchaud, Eric ePeyrin, Corinne eRavelet   +4 more
doaj   +1 more source

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source