Results 41 to 50 of about 6,923 (171)

A structural study of the myristoylated N-terminus of ARF1

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2005
The effect of myristoylation on the 15-amino-acid peptide from the membrane-binding N-terminus of ADP ribosylation factor 1 (ARF1) was studied using neutron diffraction and circular dichroism. A previous study on the non-acylated form indicated that the peptide lies parallel to the membrane, at a shallow depth and in the vicinity of the ...
Harroun, Thad, Bradshaw, Jeremy, Balali-Mood, Kia, Katsaras, John   +3 more
openaire   +3 more sources

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. [PDF]

PLoS Biology, 2005
Semliki forest virus (SFV) is internalized by clathrin-mediated endocytosis, and transported via early endosomes to late endosomes and lysosomes. The intracellular pathway taken by individual fluorescently labeled SFV particles was followed using ...
Andreas Vonderheit, Ari Helenius
doaj   +2 more sources

CRISPR/Cas9‐Mediated Knockout of LbSP1 and LbSP5G1 Reveals Efficient Customization of Shoot Architecture in Black Goji Berry

Plant Biotechnology Journal, EarlyView.
ABSTRACT Goji is recognized as a nutritionally rich superfruit with medicinal and industrial importance. However, its inherently indeterminate growth habit causes asynchronous flowering and uneven fruit ripening, resulting in harvesting inefficiency with high labour costs and low fruit yield.
Fazal Rehman, Huanfang Liu, Yun Ma, Shaohua Zeng, Yan Wu, Yuan Zong, Chao Yang, Ying Wang   +7 more
wiley   +1 more source

Mutational Analysis of the Arf1•GTP/Arf GAP Interface Reveals an Arf1 Mutant that Selectively Affects the Arf GAP ASAP1 [PDF]

Current Biology, 2005
Arf1 is a GTP binding protein that functions at a number of cellular sites to control membrane traffic and actin remodeling. Arf1 is regulated by site-specific GTPase-activating proteins (GAPs). The combined results of crystallographic and biochemical studies have led to the proposal that Arf1 GAPs differ in the specific interface formed with Arf1.
Luo, Ruibai, Jacques, Kerry, Ahvazi, Bijan, Stauffer, Stacey, Premont, Richard T., Randazzo, Paul A.   +5 more
openaire   +2 more sources

Understanding extracellular vesicle biology, isolation, and characterization: The theory of EV‐erything

Periodontology 2000, EarlyView.
Abstract Background Extracellular vesicles (EVs), especially exosomes, are nanoparticles increasingly recognized as key regulators of intercellular communication in both physiological and pathological aspects. Despite rapid progress, inconsistencies in nomenclature, isolation, and characterization continue to restrict translational advancement.
Paras Ahmad, Nima Farshidfar, Nathan E. Estrin, Yulan Wang, Yufeng Zhang, Anton Sculean, Richard J. Miron   +6 more
wiley   +1 more source

The small GTPase ARF6 regulates GABAergic synapse development

Molecular Brain, 2020
ADP ribosylation factors (ARFs) are a family of small GTPases composed of six members (ARF1–6) that control various cellular functions, including membrane trafficking and actin cytoskeletal rearrangement, in eukaryotic cells.
Hyeonho Kim, Hyeji Jung, Hyunsu Jung, Seok-Kyu Kwon, Jaewon Ko, Ji Won Um   +5 more
doaj   +1 more source

Maternal protein restriction programs the ageing kidney: Proteomic signatures of early‐life origin renal and metabolic dysfunction

The Journal of Physiology, EarlyView.
Abstract figure legend Maternal protein restriction (MPR) induced persistent renal histopathological alterations accompanied by elevated serum creatinine levels. Nephron‐segment proteomic analysis revealed region‐specific molecular dysregulation affecting Bowman's capsule (PARK7, oxidative stress sensor; MSN, cytoskeletal organization), the proximal ...
Marina Pereira Pires, Matheus Naia Fioretto, Marcel Rodrigues Ferreira, Isabelle Tenori Ribeiro, Luísa Annibal Barata, Flávia Alessandra Maciel, Lucas Sobrinho Lemos, Renato Mattos, Luiz Marcos Frediane Portela, Patrick Vieira de Souza, Luisa Correia Gomes, Gustavo Monezzi Cordeiro, Wellerson Rodrigo Scarano, Patrícia Aline Boer, Jose Antonio Rocha Gontijo, Luis Antonio Justulin   +15 more
wiley   +1 more source

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.

PLoS ONE, 2014
Juvenile Batten disease (juvenile neuronal ceroid lipofuscinosis, JNCL) is a devastating neurodegenerative disease caused by mutations in CLN3, a protein of undefined function.
Mark L Schultz, Luis Tecedor, Colleen S Stein, Mark A Stamnes, Beverly L Davidson   +4 more
doaj   +1 more source

The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System

Frontiers in Cell and Developmental Biology, 2021
Arf-family small GTPases are essential protein components for membrane trafficking in all eukaryotic endomembrane systems, particularly during the formation of membrane-bound, coat protein complex-coated transport carriers.
Kana Fujibayashi, Joji Mima
doaj   +1 more source