Results 161 to 170 of about 44,168 (193)
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Molecular characterization of the murine argininosuccinate synthetase locus
Gene, 1991The cDNA and gene encoding murine argininosuccinate synthetase were cloned and characterized. The cDNA sequence predicts a peptide of 412 amino acids (aa) including the initiator methionine. There is 98% identity with the aa sequence of the human enzyme. The 3'-untranslated region of the cDNA includes two regions of sequence which are conserved between
L C, Surh, A L, Beaudet, W E, O'Brien
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Experimental Cell Research, 1984
Human fibroblasts deficient in either argininosuccinate synthetase or argininosuccinate lyase show low levels of incorporation of [14C]citrulline into protein. However, when these two cell types are co-cultured [14C]citrulline incorporation is restored to the levels found in control cultures.
J S, Davidson +2 more
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Human fibroblasts deficient in either argininosuccinate synthetase or argininosuccinate lyase show low levels of incorporation of [14C]citrulline into protein. However, when these two cell types are co-cultured [14C]citrulline incorporation is restored to the levels found in control cultures.
J S, Davidson +2 more
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Long-term survival of patients with argininosuccinate synthetase deficiency
The Journal of Pediatrics, 1995To monitor long-term survival and outcome of patients with neonatal-onset argininosuccinate synthetase deficiency (ASD) who were treated with specific therapeutic protocols designed to activate alternative pathways of waste nitrogen excretion.Patients for this study included 24 infants born before 1990 and rescued from hyperammonemic coma caused by ...
N E, Maestri +2 more
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Arginine deprivation and argininosuccinate synthetase expression in the treatment of cancer
International Journal of Cancer, 2010AbstractArginine, a semi‐essential amino acid in humans, is critical for the growth of human cancers, particularly those marked by de novo chemoresistance and a poor clinical outcome. In addition to protein synthesis, arginine is involved in diverse aspects of tumour metabolism, including the synthesis of nitric oxide, polyamines, nucleotides, proline ...
Delage, B +6 more
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Clearance of amino acids by hemodialysis in argininosuccinate synthetase deficiency
The Journal of Pediatrics, 2004We determined the dialytic clearance of amino acids involved in ammoniagenesis and nitrogen excretion in a neonate with argininosuccinate synthetase deficiency who underwent acute hemodialysis. Plasma ammonia and plasma and dialysate amino acid concentrations were obtained at baseline, 30-minute intervals during hemodialysis, and 30 minutes after the ...
Kevin D, McBryde +5 more
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Kinetic analysis of argininosuccinate synthetase in a variant form of citrullinaemia
Journal of Inherited Metabolic Disease, 1982AbstractArgininosuccinate (ASA) synthetase in the liver from a patient with a variant form of citrullinaemia was analysed. Serum citrulline level was approximately 40 times higher than the control level, and the ASA synthetase activity was approximately 10% that of the control. Other urea cycle enzymes were within normal ranges.
I, Akaboshi +3 more
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A radiochemical assay for argininosuccinate synthetase with [U-14C]aspartate
Analytical Biochemistry, 1983A simple and sensitive radiochemical procedure to assay argininosuccinate synthetase activity in crude tissue homogenates and lysates of cultured cells is described. The new method depends on the location of 14C, uniformly, in the four carbons of aspartate.
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[34] Argininosuccinate synthetase (steer liver)
1970Publisher Summary This chapter discusses the assay, purification, and properties of argininosuccinate synthetase. The principle of assay method state that the formation of L-aspartate ligase (AMP) in the forward reaction is coupled to the oxidation of diphosphopyridine nucleotide (DPNH) through lactic dehydrogenase and an adenosine triphosphate (ATP)
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Isolation and characterization of argininosuccinate synthetase from human liver
Biochemistry, 1979This communication describes the purification and characterization of argininosuccinate synthetase from human liver. By numerous criteria including electrophoresis in sodium dodecyl sulfate containing gels, electrophoresis in nondissociating gels, and analytical ultracentrifugation, the protein is homogeneous at a specific activity of 4.2 mumol/(min mg)
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Citrullinemia, argininosuccinate synthetase deficiency
Cytogenetics and Cell Genetics, 2008E. Spector +4 more
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