Results 151 to 160 of about 7,063 (180)

Artificial oocyte activation after intracytoplasmic morphologically selected sperm injection: A prospective randomized sibling oocyte study

Human Fertility, 2016
This study aimed to evaluate the effect of artificial oocyte activation (AOA) by calcium ionophore after intracytoplasmic morphologically selected sperm injection (IMSI) on fertilization, cleavage rate and embryo quality. A total of 194 oocytes from 21 cycles from women with a history of low fertilization rate accompanying teratozoospermia were ...
Batu, Aydinuraz, Enver Kerem, Dirican, Safak, Olgan, Ozlem, Aksunger, Onur Kadir, Erturk   +4 more
openaire   +2 more sources

Culture conditions affect Ca2+ release in artificially activated mouse and human oocytes

Reproduction, Fertility and Development, 2018
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of
Yuechao, Lu, Davina, Bonte, Minerva, Ferrer-Buitrago, Mina, Popovic, Jitesh, Neupane, Margot, Van der Jeught, Luc, Leybaert, Petra, De Sutter, Björn, Heindryckx   +8 more
openaire   +2 more sources

Artificial Oocyte Activation to Improve Blastocyst Development

Reproductive BioMedicine Online, 2022
S. Montgomery, S. Boardman, A. Campbell
openaire   +1 more source

Reconstruction of artificial nuclei with nuclear import activity in living mouse oocytes

Genes to Cells
Abstract In eukaryotes, DNA is housed within the cell nucleus. Molecules required for the formation of a nucleus have been identified using in vitro systems with frog egg extracts and in vivo imaging of somatic cells. However, little is known about the physicochemical factors and conditions required for nuclear formation in mouse ...
Nao Yonezawa, Tomoko Shindo, Haruka Oda, Hiroshi Kimura, Yasushi Hiraoka, Tokuko Haraguchi, Kazuo Yamagata   +6 more
openaire   +2 more sources

Effect of high fat diet on artificial oocyte activation following superovulation in mice

Zygote, 2015
SummaryThe aim of the present study was to determine the effects of increased dietary intake and high fat diet (HFD) in mice on artificial oocyte activation by using puromycin or roscovitine. Six-week-old mice were fed as either a control diet group, an increased dietary intake group or an HFD group for 4 weeks.
Daisuke, Yamamoto, Toshiyuki, Yasui, Chika, Kobayashi, Takane, Kitazato, Takeshi, Iwasa, Minoru, Irahara   +5 more
openaire   +2 more sources

Comparison of clinical artificial oocyte activation protocols on mouse egg activation and embryo development

Reproduction
In brief Artificial oocyte activation (AOA) with Ca 2+ ionophores is a valuable tool in assisted reproduction but may affect cellular events critical for embryo development.
Lucas N González, Valeria Sulzyk, Patricia S Cuasnicú, Débora J Cohen   +3 more
openaire   +2 more sources

Effect of Artificial Oocyte Activation by Calcium Ionophore on One-Day-Old Unfertilized Oocytes after ICSI

Journal of Mammalian Ova Research, 2015
Abstract: At our clinic, a successfully fertilized human oocyte is defined as one with two polar bodies and two pronuclei (PN) 18 h after ICSI, but when some oocytes do not form PN. Recently, a successful pregnancy and live birth following artificial oocyte activation (AOA; using Ca2+ ionophore) of ICSI-treated one-day-old unfertilized oocytes was ...
Masahiro Sakurai, Shinichi Watanabe, Toyomi Tanaka, Rie Matsunaga, Naoko Yamanaka, Minako Kamihata, Chikako Kani, Akiko Kuwahata, Masanori Ochi, Toshitaka Horiuchi   +9 more
openaire   +1 more source

[A control study of artificial oocyte activation in the same period].

Zhonghua yi xue za zhi, 2017
Objective: To compare the fertility rate and embryo outcome between normal fertilization and the use of Calcium ionophore A23187 on the same period of the same cycle of human ovum for artificial activation. Methods: Patients who conducted the intracytoplasmic sperm injection (ICSI) cycle in vitro fertilization assisted reproductive from January 2015 to
D Y, Hao, R, Zhang, X, Zhang, C Y, Shen, Y, Yang, W B, Wu, Y L, Liu, J, Liu, J R, Zhang, X L, Wang   +9 more
openaire   +1 more source

Intracellular Ca2+ during fertilization and artificial activation in mouse oocytes.

Zhongguo yao li xue bao = Acta pharmacologica Sinica, 1999
To study the mechanism of oocyte activation in mammals.Mouse oocytes arrested at metaphase of the second meiotic division were loaded with Fura 2-AM and then activated with ethanol, calcimycin, electric pulse or fertilization. Intracellular free Ca2+ during activation were measured by Spex AR-CM-MIC cation system.
M Q, Deng, B Q, Fan
openaire   +1 more source

Artificial oocyte activation with ionomycin compared with A23187 among patients at risk of failed or impaired fertilization

Reproductive BioMedicine Online, 2023
Lei Jia, Peigen Chen, Wenlong Su
exaly