Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application. [PDF]
Hum Reprod Open, 2022 AbstractBACKGROUNDOocyte activation deficiency (OAD) is attributed to the majority of cases underlying failure of ICSI cycles, the standard treatment for male factor infertility. Oocyte activation encompasses a series of concerted events, triggered by sperm-specific phospholipase C zeta (PLCζ), which elicits increases in free cytoplasmic calcium (Ca2+)
Kashir J, Ganesh D, Jones C, Coward K.
europepmc +7 more sources
Assisted gamete treatment to pinpoint acquired meiotic maturity and overcome oocyte activation deficiency contributed by both gametes [PDF]
F&S Reports, 2023 Objective: To treat couples with total fertilization failure (TFF) based on a combined oocyte- and sperm-related oocyte activation deficiency by optimizing oocyte response to chemical activation with calcium ionophore. Design: Case report.
Philip Xie, B.Sc. +6 more
doaj +2 more sources
Assisted Oocyte Activation following Intracytoplasmic Sperm Injection: A Sensible Option for Infertile Couples with Severe Teratozoospermia [PDF]
International Journal of Fertility and Sterility, 2023 The intracytoplasmic sperm injection (ICSI) has significantly improved male factor infertility treatment; however, complete fertilization failure still occurs in 1-5% of ICSI treatment cycles mainly due to oocyte activation failure.
Marziyeh Tavalaee +3 more
doaj +2 more sources
Phospholipase C zeta: a hidden face of sperm for oocyte activation and early embryonic development [PDF]
Obstetrics & Gynecology ScienceOocyte activation is a fundamental event in mammalian fertilization and is initiated by a cascade of calcium signaling and oscillation pathways. Phospholipase C zeta (PLCζ) is involved in modulating cortical granule exocytosis, releasing oocyte meiotic ...
Soukaina Azil +4 more
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The Therapeutic and Diagnostic Potential of Phospholipase C Zeta, Oocyte Activation, and Calcium in Treating Human Infertility [PDF]
Pharmaceuticals, 2023 Oocyte activation, a fundamental event during mammalian fertilisation, is initiated by concerted intracellular patterns of calcium (Ca2+) release, termed Ca2+ oscillations, predominantly driven by testis-specific phospholipase C zeta (PLCζ).
Haia M. R. Abdulsamad +7 more
doaj +2 more sources
Chloroquine inhibits artificial oocyte activation induced by ethanol or Sr2+ but not by sperm in mice [PDF]
The Journal of Reproduction and DevelopmentCalcium release from the endoplasmic reticulum via sperm-derived phospholipase C zeta is crucial for oocyte activation during fertilization. Chloroquine (CQ) inhibits the increase in cytoplasmic calcium.
Tadashi YAMAZAKI +2 more
doaj +2 more sources
Sperm-oocyte interplay: an overview of spermatozoon’s role in oocyte activation and current perspectives in diagnosis and fertility treatment [PDF]
Cell & Bioscience, 2021 The fertilizing spermatozoon is a highly specialized cell that selects from millions along the female tract until the oocyte. The paternal components influence the oocyte activation during fertilization and are fundamental for normal embryo development ...
Mohammad Ishraq Zafar +2 more
doaj +2 more sources
Effects of assisted oocyte activation with calcium- ionophore and strontium chloride on in vitro ICSI outcomes [PDF]
Iranian Journal of Basic Medical Sciences, 2018 Objective(s): Failed fertilization after intra-cytoplasmic sperm injection (ICSI) is mainly attributed to failed oocyte activation and can be overcome by artificial oocyte activation (AOA).
Marziyeh Norozi-Hafshejani +4 more
doaj +2 more sources
Oocyte activation with phospholipase Cζ mRNA induces repetitive intracellular Ca2+ rises and improves the quality of pig embryos after intracytoplasmic sperm injection [PDF]
The Journal of Reproduction and DevelopmentFor the intracytoplasmic sperm injection (ICSI) procedure in pigs, an electrical pulse (EP) has been used as an effective method for oocyte stimulation, but unlike sperm, EP is unable to induce Ca2+ oscillations.
Michiko NAKAI +4 more
doaj +2 more sources
Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia [PDF]
Frontiers in Endocrinology, 2020 Fertilization failure is common in patients with round-headed sperm, a form of globozoospermia. Artificial oocyte activation is able to assist oocyte fertilization after sperm injection in these patients.
Xiangli Niu +3 more
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