Results 61 to 70 of about 9,601 (189)

African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified Antigen (ASFV CP-Ag)

, 2022
The present chapter describes a simple and economic indirect enzyme immunoassay (ELISA ) for African swine fever virus (ASFV) antibody detection based on the use of the soluble cytoplasmic fraction of ASFV-infected monkey stable cells (MS). The soluble antigen proteins of ASFV-infected cells are separated by sucrose precipitation centrifugation, and ...
Carmina, Gallardo, Nieto, R, Arias, M
openaire   +3 more sources

African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge

Viruses, 2023
ASFV vaccine candidate ASFV-G-ΔI177L has been shown to be highly efficacious in inducing protection against challenges with the parental virus, the Georgia 2010 isolate, as well as against field strains isolated from Vietnam. ASFV-G-ΔI177L has been shown
Manuel V. Borca, Ayushi Rai, Nallely Espinoza, Elizabeth Ramirez-Medina, Edward Spinard, Lauro Velazquez-Salinas, Alyssa Valladares, Ediane Silva, Leeanna Burton, Amanda Meyers, Cyril G. Gay, Douglas P. Gladue   +11 more
doaj   +1 more source

Development and In Vivo Evaluation of a MGF110-1L Deletion Mutant in African Swine Fever Strain Georgia

Viruses, 2021
African swine fever (ASF) is currently causing an epizootic, affecting pigs throughout Eurasia, and causing significant economic losses in the swine industry.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Ediane Silva, Nallely Espinoza, James Zhu, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue   +9 more
doaj   +1 more source

Function investigation of p11.5 in ASFV infection

Virologica Sinica
Virus replication relies on complex interactions between viral proteins. In the case of African swine fever virus (ASFV), only a few such interactions have been identified so far. In this study, we demonstrate that ASFV protein p72 interacts with p11.5 using co-immunoprecipitation and liquid chromatography-mass spectrometry (LC-MS).
Dan Yin, Bin Shi, Renhao Geng, Yingnan Liu, Lang Gong, Hongxia Shao, Kun Qian, Hongjun Chen, Aijian Qin   +8 more
openaire   +2 more sources

Deletion of an African Swine Fever Virus ATP-Dependent RNA Helicase QP509L from the Highly Virulent Georgia 2010 Strain Does Not Affect Replication or Virulence

Viruses, 2022
African swine fever virus (ASFV) produces a lethal disease (ASF) in domestic pigs, which is currently causing a pandemic deteriorating pig production across Eurasia.
Elizabeth Ramirez-Medina, Elisabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Edward Spinard, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue   +10 more
doaj   +1 more source

The African Swine Fever Virus with MGF360 and MGF505 Deleted Reduces the Apoptosis of Porcine Alveolar Macrophages by Inhibiting the NF-κB Signaling Pathway and Interleukin-1β

Vaccines, 2021
African swine fever virus (ASFV) poses serious threats to the swine industry. The mortality rate of African swine fever (ASF) is 100%, and there is no effective vaccine currently available.
Qi Gao, Yunlong Yang, Weipeng Quan, Jiachen Zheng, Yizhuo Luo, Heng Wang, Xiongnan Chen, Zhao Huang, Xiaojun Chen, Runda Xu, Guihong Zhang, Lang Gong   +11 more
doaj   +1 more source

Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities [PDF]

, 2004
African swine fever virus (ASFV), a large icosahedral deoxyvirus, is the causative agent of an economically relevant hemorrhagic disease that affects domestic pigs.
Eulálio, A., Nunes-Correia, I., Carvalho, A. L., Faro, C., Citovsky, V., Simões, S., Lima, M. C. Pedroso de   +6 more
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Temporally integrated transcriptome analysis reveals ASFV pathology and host response dynamics

Frontiers in Immunology, 2022
African swine fever virus (ASFV) causes a lethal swine hemorrhagic disease and is currently responsible for widespread damage to the pig industry. The pathogenesis of ASFV infection and its interaction with host responses remain poorly understood. In this study, we profiled the temporal viral and host transcriptomes in porcine alveolar macrophages ...
Lin Lv, Lin Lv, Tianyun Zhang, Hanying Jia, Yanyan Zhang, Asif Ahsan, Xiaoyang Zhao, Teng Chen, Zhiqiang Shen, Zhiqiang Shen, Ning Shen, Ning Shen   +11 more
openaire   +3 more sources

Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs

Viruses, 2022
Infection of pigs with the African swine fever virus (ASFV) leads to a devastating hemorrhagic disease with a high mortality of up to 100%. In this study, a CD2v gene deletion was introduced to a genotype IX virus from East Africa, ASFV-Kenya-IX-1033 ...
Johanneke D. Hemmink, Emmanuel M. Khazalwa, Hussein M. Abkallo, Bernard Oduor, Jeremiah Khayumbi, Nicholas Svitek, Sonal P. Henson, Sandra Blome, Günther Keil, Richard P. Bishop, Lucilla Steinaa   +10 more
doaj   +1 more source

Viral modulation of interferon (IFN) responses to african swine fever virus (ASFV) [PDF]

, 2011
Tese de mestrado. Biologia (Biologia Humana e Ambiente). Universidade de Lisboa, Faculdade de Ciências, 2011A imunidade inata constitui a primeira resposta dada por um hospedeiro quando atacado por agentes patogénicos. A resposta imune baseia-se em genes
Costa, Emanuel Nery de Oliveira Quartin, 1986-
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