Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase
A potential vaccine candidate, Necator americanus secretory protein (Na-ASP1), against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1, a 45 kDa protein containing 20 cysteines, was directed outside the cell by fusing the protein to ...
İNAN, MEHMET +3 more
core +1 more source
Expression of Aspergillus oryzae asparaginase in yeast Pichia pastoris
Total 13 rare codons in gene encoding asparaginase from Aspergillus oryzae were replaced with the most frequent ones for Pichia pastoris. The optimized gene (asp1) was synthesized and cloned into pUC57 then inserted into pPIC9 at XhoI-NotI cleavage site ...
Nguyen Thanh Ngoc +11 more
core +1 more source
Effect of Aspartic Acid and Glycine on Calcite Growth
Organic molecules control calcite growth and crystal morphology, influence biomineralization processes, and offer clues for optimizing antiscalants for industry.
G. Montanari (3070848) +6 more
core +1 more source
Kinase II-dependent formation of angiotensins II and III in the hepatic circulation
Experiments were performed in 14 pentobarbital-anesthetized dogs to 1) determine if the hepatic arterial vasoconstrictor effects of [des-Asp1]angiotensin I [( des-Asp1]ANG I) were due to its local conversion to angiotensin III (ANG III) and 2) to ...
S. L. Britton +3 more
core +1 more source
Enhancement of Protein Secretion in \u3cem\u3ePichia pastoris\u3c/em\u3e by Overexpression of Protein Disulfide Isomerase [PDF]
A potential vaccine candidate, Necator americanus secretory protein (Na-ASP1), against hookworm infections, has been expressed in Pichia pastoris. Na- ASP1, a 45 kDa protein containing 20 cysteines, was directed outside the cell by fusing the protein to ...
Aryasomayajula, Dinesh +3 more
core
Vasodepressor action of angiotensin in conscious chickens
In chronically cannulated conscious chickens, Gallus gallus, native chicken angiotensin II ([Asp1,Val5]ANG II) caused biphasic blood pressure responses, a depressor followed by a pressor response. The pressor response appears to be mediated primarily by
Y. Nakamura, H. Nishimura, M. C. Khosla
core +1 more source
Interphase MT dynamics in mal3Δ variant strains.
Parameters of MT dynamics were measured for the indicated strains expressing nmt81::gfp-atb2+. Cells were grown in non-selective minimal medium. n, number of MTs measured; asterisks denote significance between mal3Δ and mal3Δ asp1H397A; ↑, parameter is ...
Reinhard Fischer (182192) +11 more
core +1 more source
Many pathogenic bacteria, includingStreptococcus gordonii, possess a pathway for the export of a single serine-rich-repeat protein that mediates the adhesion of bacteria to host cells and the extracellular matrix.
Maofu Liao +10 more
core +1 more source
Toward AI-Assisted Methodological Evaluations of Health Risk Analyses Using Diverse Types of Evidence. [PDF]
Cox LA.
europepmc +1 more source
The Effect of Cu<sup>2+</sup> and Zn<sup>2+</sup> Ions' Nonbonded Interactions on the Aggregation of β-Amyloid 1-16 and 25-35 Fragments─A Molecular Dynamics Simulation Study. [PDF]
Costa RKM +4 more
europepmc +1 more source

