Results 31 to 40 of about 59,687 (251)

Midostaurin Reverses ABCB1-Mediated Multidrug Resistance, an in vitro Study

Frontiers in Oncology, 2019
Overexpression of ABC transporters in cancer cells is an underlying mechanism of multidrug resistance (MDR), leading to insensitive response to chemotherapeutic strategies. Thus, MDR is often results in treatment failure in the clinic.
Ning Ji, Ning Ji, Yuqi Yang, Chao-Yun Cai, Jing-Quan Wang, Zi-Ning Lei, Zhuo-Xun Wu, Qingbin Cui, Dong-Hua Yang, Zhe-Sheng Chen, Dexin Kong, Dexin Kong   +11 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Single-molecule visualization of conformational changes and substrate transport in the vitamin B12 ABC importer BtuCD-F

Nature Communications, 2017
The vitamin B12 importer BtuCD-F is a type II ATP-binding cassette transporter. Here the authors use single-molecule fluorescence techniques to analyse ATP hydrolysis and substrate transport in individual BtuCD-F complexes embedded in liposomes, and ...
Joris M. H. Goudsmits, Dirk Jan Slotboom, Antoine M. van Oijen   +2 more
doaj   +1 more source

Conformational motion of the ABC transporter MsbA induced by ATP hydrolysis.

PLoS Biology, 2007
We measured the amplitude of conformational motion in the ATP-binding cassette (ABC) transporter MsbA upon lipopolysaccharide (LPS) binding and following ATP turnover by pulse double electron-electron resonance and fluorescence homotransfer. The distance
Peter P Borbat, Kavitha Surendhran, Marco Bortolus, Ping Zou, Jack H Freed, Hassane S Mchaourab   +5 more
doaj   +1 more source

ATP-binding cassette transporters in Escherichia coli

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2008
ATP-binding cassette (ABC) transporters are integral membrane proteins that actively transport molecules across cell membranes. In Escherichia coli they consist primarily of import systems that involve in addition to the ABC transporter itself a substrate binding protein and outer membrane receptors or porins, and a number of transporters with varied ...
Moussatova, Anastassiia, Kandt, Christian, O'Mara, Megan L., Tieleman, D. Peter   +3 more
openaire   +5 more sources

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Characterization of bacteriocin ABC transporter ATP-binding protein produced by a newly isolated Enterococcus casseliflavus MI001 strain

Beni-Suef University Journal of Basic and Applied Sciences, 2019
Background ATP-binding cassette (ABC) transporters constitute one of the largest transporter protein families and play a role in diverse biological processes.
Indira Mikkili, Venkateswarulu TC, Abraham Peele Karlapudi, Vidya Prabhakar Kodali, Krupanidhi Srirama   +4 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Engineered Microvascular Model of the Blood–Brain–Tumor Barrier Reveals Endothelial Remodeling in Diffuse Midline Glioma

Advanced Healthcare Materials, EarlyView.
We developed a patient‐derived, functional microfluidic model of the diffuse midline glioma (DMG) blood–brain–tumor barrier (BBTB) comprised of endothelial cells, astrocytes, pericytes, and tumor cells. The system forms perfusable microvasculature, reveals the BBTB retains vascular integrity, identifies DMG‐specific transcriptomic changes distinct from
Kimberly R. Bennett, Yann S. Vanrobaeys, Tessy Teboh, Zakiya Smith, Anderson Scott, Cynthia Hajal, Paula T. Hammond, Joelle P. Straehla   +7 more
wiley   +1 more source

Inward- and outward-facing X-ray crystal structures of homodimeric P-glycoprotein CmABCB1

Nature Communications, 2019
P-glycoprotein, an ATP-binding cassette (ABC) transporter, extrudes a large variety of xenobiotics from the cell which protects tissues from toxins.
Atsushi Kodan, Tomohiro Yamaguchi, Toru Nakatsu, Keita Matsuoka, Yasuhisa Kimura, Kazumitsu Ueda, Hiroaki Kato   +6 more
doaj   +1 more source