Results 231 to 240 of about 1,031,034 (270)

Automated Planar Patch-Clamp

2013
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as ...
Carol J, Milligan, Clemens, Möller
openaire   +2 more sources

Automated Patch Clamping Using the QPatch

2009
Whole-cell voltage clamp electrophysiology using glass patch pipettes (1) is regarded as the gold standard for measurement of compound activity on ion channels. Despite the high quality of the data generated by this method, in its traditional format, patch clamping has limited use in drug screening due to very low throughput.
Kenneth A, Jones, Nicoletta, Garbati, Hong, Zhang, Charles H, Large   +3 more
openaire   +2 more sources

An automated planar patch-clamp approach to measure the membrane potential and resting membrane currents in a human cerebrovascular endothelial cell line

Journal of Neuroscience Methods
Background: The conventional “whole-cell patch-clamp” recording technique is widely used to measure the resting membrane potential (VM) and to dissect the underlying membrane ionic conductances in isolated vascular endothelial cells.
Giovambattista De Sarro, Teresa Soda, Francesco Moccia   +2 more
exaly   +2 more sources

QPatch: the past, present and future of automated patch clamp

Expert Opinion on Therapeutic Targets, 2006
The QPatch 16 significantly increases throughput for gigaseal patch clamp experiments, making direct measurements in ion channel drug discovery and safety testing feasible. Released to the market in the Autumn of 2004 by Sophion Bioscience, the QPatch originated from work done at NeuroSearch (Denmark) in the early days of automated patch clamp.
exaly   +3 more sources

Microchip Technology for Automated and Parallel Patch‐Clamp Recording

Small, 2006
AbstractThe patch‐clamp technique is the state‐of‐the‐art technology for the study of a large class of membrane proteins called ion channels. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets.
Brüggemann, Andrea, Stoelzle-Feix, Sonja, George, Michael, Behrends, Jan C., Fertig, Niels   +4 more
openaire   +3 more sources

Advances in the automation of whole-cell patch clamp technology

Journal of Neuroscience Methods, 2019
Electrophysiology is the study of neural activity in the form of local field potentials, current flow through ion channels, calcium spikes, back propagating action potentials and somatic action potentials, all measurable on a millisecond timescale. Despite great progress in imaging technologies and sensor proteins, none of the currently available tools
Ho-Jun, Suk, Edward S, Boyden, Ingrid, van Welie   +2 more
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Ion channel screening – automated patch clamp on the rise

Drug Discovery Today: Technologies, 2008
Ion channel proteins are of major importance for the human physiology and thus highly attractive molecular drug targets. Large-scale ion channel screening of wanted and unwanted drug effects is required, but has been limited by the lack of adequate screening technology, because available methods put a trade-off between high-throughput and high ...
Cecilia Farre, Michael George, Andrea Brüggemann, Niels Fertig   +3 more
openaire   +2 more sources

Automated ion channel screening: patch clamping made easy

Expert Opinion on Therapeutic Targets, 2007
Efficient high resolution techniques are required for screening efforts and research targeting ion channels. The conventional patch clamp technique, a high resolution but low efficiency technique, has been established for 25 years. Recent advances have opened up new possibilities for automated patch clamping.
Cecilia, Farre, Sonja, Stoelzle, Claudia, Haarmann, Michael, George, Andrea, Brüggemann, Niels, Fertig   +5 more
openaire   +2 more sources

Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology

2020
The automated patch clamp (APC) technology is used for increasing the data throughput of electrophysiological measurements, especially in safety pharmacology and drug discovery. Typically, electrical access to the cells are obtained using standard whole-cell formation by rupturing the membrane, thereby causing a rapid washout of cytosolic components ...
Kadla R, Rosholm, Kim, Boddum, Anders, Lindquist   +2 more
openaire   +2 more sources

Study of TRP Channels by Automated Patch Clamp Systems

2010
Ion channels are responsible for the permeation of ions across the membrane and their central role in cellular physiology is well established. Historically, the direct study of ion channels has been considered technically challenging. As such, a significant barrier to drug discovery for ion channels has been the low throughput of high quality ...
Morten, Sunesen, Rasmus B, Jacobsen
openaire   +2 more sources