Results 71 to 80 of about 1,031,034 (270)

The Natural Product Corramycin Acts as a DNA Gyrase Poison and Overcomes Fluoroquinolone Resistance in Mycobacterium tuberculosis

Advanced Science, EarlyView.
Corramycin, a myxobacterial natural product antibiotic, exhibits potent bactericidal activity against multidrug‐resistant Mycobacterium tuberculosis. The compound hijacks bacterial transporters such as BacA and OppABCD to enter the cell and inhibits DNA synthesis through a previously unrecognized mode of DNA gyrase poisoning, locking the enzyme in an ...
Franziska Fries, Jennifer Herrmann, Michael Dal Molin, Yaëlle Wormser, Timo Risch, Yunsheng Gao, Gareth Prosser, Francesca Gubellini, Ariel Mechaly, Joshua B. Wallach, Anthony Castro, Arka Banerjee, Sari Rasheed, Alexander Horn, Mathias Müsken, Michael Kurz, Lindsay Sonnenkalb, Viola Dreyer, Stefan Niemann, Sönke Andres, Dirk Schnappinger, Uli Kazmaier, Norbert Reiling, Rolf Müller, Jan Rybniker, Stéphanie Petrella   +25 more
wiley   +1 more source

Toh_Supp_Fig_1 – Supplemental material for Application of High-Throughput Automated Patch-Clamp Electrophysiology to Study Voltage-Gated Ion Channel Function in Primary Cortical Cultures

, 2020
Supplemental material, Toh_Supp_Fig_1 for Application of High-Throughput Automated Patch-Clamp Electrophysiology to Study Voltage-Gated Ion Channel Function in Primary Cortical Cultures by May Fern Toh, Julie M. Brooks, Tim Strassmaier, Rodolfo J. Haedo,
May Fern Toh (7988918), Sokhom S. Pin (2422621), Julie M. Brooks (5412824), Rodolfo J. Haedo (1884844), Corey B. Puryear (8387415), Tim Strassmaier (8387412), Kosalvisal Ouk (8387421), Bruce L. Roth (8387418)   +7 more
core   +1 more source

A Robotic Testing Platform for Pipelined Discovery of Resilient Dielectric Elastomer Actuators

Advanced Science, EarlyView.
This work introduces a robotic testing platform enabling high‐throughput, multi‐parameter lifetime mapping of linear dielectric elastomer actuators (DEAs). By integrating programmable high‐voltage control and automated electromechanical measurements, the system identifies material and operating conditions that double actuator lifetime.
Ang (Leo) Li, Alexander Yin, Alexander White, Sahib Sandhu, Anatol Gogoj, Denise Maria de Andrade, Matthew Francoeur, Victor Jimenez‐Santiago, Van Remenar, Codrin Tugui, Mihai Duduta   +10 more
wiley   +1 more source

Cell segmentation and pipette identification for automated patch clamp recording [PDF]

Robotics and Biomimetics, 2014
Abstract A visual-based approach for identifying living cells and performing the automated patch clamp recording was reported. Based on the image processing and blob detection algorithm, the vision-based method was developed for the detection and identification of biological cells and micropipette.
Yang, Runhuai, Tam, Chi Ho, Cheung, Ka Lun, Wong, Kai Chun, Xi, Ning, Yang, Jie, Lai, King Wai Chiu   +6 more
openaire   +1 more source

Supplemental_Material_for_Optopatch_VS_IWB_Nav1.7_screen_final – Supplemental material for Correlation of Optical and Automated Patch Clamp Electrophysiology for Identification of Na_V1.7 Inhibitors

, 2020
Supplemental material, Supplemental_Material_for_Optopatch_VS_IWB_Nav1.7_screen_final for Correlation of Optical and Automated Patch Clamp Electrophysiology for Identification of NaV1.7 Inhibitors by Hongkang Zhang, Bryan D.
Chris M. Hempel (8719047), Bryan D. Moyer (261804), Vivian C. Hecht (8719038), Ryan J. Babcock (8719041), Graham T. Dempsey (1267890), Joseph G. McGivern (6332135), Kevin Dong (8719044), Owen B. McManus (2055892), Hongkang Zhang (8719035), Violeta Yu (2013523), Christopher A. Werley (504796), Michael Jarosh (2424730)   +11 more
core   +1 more source

Machine Learning of Temperature‐Dependent Chemical Kinetics Using Parallel Droplet Microreactors

Advanced Science, EarlyView.
An integrated droplet microfluidics and machine learning framework enables high‐throughput characterization of temperature‐dependent reaction kinetics. Time‐resolved measurements from thousands of droplets train Neural ODE models that accurately predict nonlinear reaction dynamics across diverse thermal environments, bridging large‐scale ...
Mamoru Saita, Yutaka Hori
wiley   +1 more source

Photobiomodulation‑Engineered Extracellular Vesicles Enhance Neural Differentiation via UFL1‑Mediated UFMylation in Spinal Cord Injury

Advanced Science, EarlyView.
Photobiomodulated microglia release engineered extracellular vesicles that deliver UFL1 to the injured spinal cord. UFL1 competitively binds p53 with MDM2, inhibiting p53 ubiquitination and stabilizing p53 signaling. This dual mechanism simultaneously promotes anti‐inflammatory microglial polarization and directs neural stem cell differentiation toward
Yunxiao Fang, Zuomeng Wu, Ruocheng Guo, Cancan Wang, Yue Qin, Yixiang Dong, Aoyun Li, Ao Liu, Chongyu Jia, Tianyu Han, Peiwen Song, Lei Chen, Cailiang Shen   +12 more
wiley   +1 more source

A range of voltage-clamp protocol designs for rapid capture of hERG kinetics [version 2; peer review: 3 approved]

Wellcome Open Research
We provide details of a series of short voltage-clamp protocols designed for gathering a large amount of information on hERG (Kv11.1) ion channel gating.
Chon Lok Lei, Dominic J Whittaker, Matthew D Perry, Monique J Windley, Gary R Mirams, Adam P Hill   +5 more
doaj   +1 more source

manual patch clamp raw.zip

, 2017
Raw data on manual patch clamp measurements are stored under manual patch clamp raw. The first detachment tests subfolder contains data on the experiments with the three Accutase, Trypsin, and Detachin solutions.
Denise Franz (3696583), jan gimsa (3696589), Hervør Lykke Olsen (3696586), Oliver Klink (3696580)   +3 more
core   +1 more source

Structural Basis of Shiga Toxin Neutralization by Human Antibodies Targeting Canonical Receptor‐Binding and Non‐Canonical Assembly Sites

Advanced Science, EarlyView.
Cryo‐electron microscopy structures reveal two distinct neutralization mechanisms of fully human monoclonal antibodies targeting the Shiga toxin 1a B subunit. RDS059 occludes the Gb3 receptor‐binding site, whereas RDS045 engages a lateral quaternary epitope to disrupt holotoxin assembly.
Jianting Ke, Xin Li, Xiaoli Wang, Zhaoxia Huang, Shaojie Xue, Yuyang Liu, Jing Pan, Guowei Hu, Yayu Wang, Cheng Wang, Shoudeng Chen   +10 more
wiley   +1 more source