Results 81 to 90 of about 1,031,034 (270)

ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research

Frontiers in Neuroinformatics, 2014
The complexity of modern neurophysiology experiments requires specialized software to coordinate multiple acquisition devices and analyze the collected data.
Luke eCampagnola, Megan B. Kratz, Paul B. Manis   +2 more
doaj   +1 more source

Antigen‐Detected NMR for Minimal Epitope Engineering and Structure‐Guided Selection of a NaV1.7‐Selective Nanobody

Advanced Science, EarlyView.
A minimal extracellular epitope from voltage‐gated sodium channel NaV1.7 was engineered into an isotope‐labelled antigen for antigen‐detected nuclear magnetic resonance (NMR). Together with AlphaFold2 (AF2), this enabled structure‐guided selection of R4C8, a human NaV1.7‐selective nanobody that labels fixed NaV1.7‐expressing cells and produces staining
Junyu Liu, Wanlin Chen, Ben Cristofori‐Armstrong, Theo Crawford, Kai‐En Chen, Peng Xie, Rainbow Wing Bo Chan, Yifei Zhu, Mimi Golder, Anneka Pereira Schmidt, Jennifer D. Naughton, Nicholas D. Condon, Åsa Andersson, Farhad Dehkhoda, Kirsten L. McMahon, Tabea Klasfauseweh, Ashvriya Thapa, Hue Tran, Poanna Tran, Sina Jami, Lotten Ragnarsson, Sebastian G. B. Furness, Jennifer R. Deuis, Brett M. Collins, Wai‐Hong Tham, Indira Prasadam, Irina Vetter, Mehdi Mobli   +27 more
wiley   +1 more source

Microfluidic device for cell screening and patch-clamp analysis

, 2012
The purpose is to realize a Lab-On-Chip based device for cell screening and electrophysiological assay to introduce the Patch-Clamp as a routine clinical test.
Valbusa U, Firpo G, Repetto L, Guida P
core   +1 more source

Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

eLife, 2016
Voltage-gated ion channels mediate electrical dynamics in excitable tissues and are an important class of drug targets. Channels can gate in sub-millisecond timescales, show complex manifolds of conformational states, and often show state-dependent ...
Hongkang Zhang, Elaine Reichert, Adam E Cohen   +2 more
doaj   +1 more source

Decoding the Neural Circuit Underlying Context‐Cue Association in Retrieval of Morphine Reward Memory

Advanced Science, EarlyView.
Contextual cues recruit the glutamatergic vCA1–dmPFC pathway to retrieve morphine reward memory by reshaping cell‐type‐specific prefrontal microcircuit activity. Distinct regulation of PV+ and SST+ interneurons defines this hippocampal–prefrontal mechanism.
Zhifeng Shi, Guangying Li, Qian Yang, Rusun Liu, Linghan Gu, Mingmei Wang, John L. Waddington, Fuhai Ji, Xuechu Zhen   +8 more
wiley   +1 more source

Konzeption eines Patch-on-Chip-Systems für Wirkstofftests und Grundlagenforschung an vernetzten adhärenten Zellen [PDF]

, 2014
Die vorliegende Arbeit befasst sich mit der Konzeption eines neuartigen Patch-Clamp-Systems, mit dem das Verhalten von adhärent wachsenden Zellnetzwerken automatisiert untersucht werden kann.
Tautorat, Carsten (gnd: 1036273342)
core   +1 more source

An Extracellular Pore‑Targeting Peptide Defines a Designable Allosteric Site in TRPV2

Advanced Science, EarlyView.
Structure‐guided peptide engineering yields Depiv2, a highly potent and subtype‐selective TRPV2 inhibitor that binds to the extracellular pore and remodels it into a closed, non‐conductive state. Depiv2 suppresses pathological cardiac hypertrophy, establishing the TRPV2 outer pore as a designable interface for selective peptide modulation.
Aiqin Zhu, Hongkun Wang, Jiawei Wang, Xiaolu Liu, Aerziguli Aierken, Lizhen Xu, Ping Liang, Fan Yang   +7 more
wiley   +1 more source

Insights into the structure and modulation of human TWIK-2

Nature Communications
The Tandem of pore domain in a Weak Inward Rectifying K+ channel 2 (TWIK-2; KCNK6) is a member of the Two-Pore Domain K + (K2P) channel family, which is associated with pulmonary hypertension, lung injury, and inflammation.
Qianqian Ma, Ciria C. Hernandez, Vikas Navratna, Arvind Kumar, Jaimin K. Rana, Jiameng Zong, Abraham Lee, Shyamal Mosalaganti   +7 more
doaj   +1 more source

A Kinetic Map of the Homomeric Voltage-Gated Potassium Channel (Kv) Family

Frontiers in Cellular Neuroscience, 2019
The voltage-gated potassium (Kv) channels, encoded by 40 genes, repolarize all electrically excitable cells, including plant, cardiac, and neuronal cells.
Rajnish Ranjan, Emmanuelle Logette, Emmanuelle Logette, Michela Marani, Mirjia Herzog, Mirjia Herzog, Valérie Tâche, Enrico Scantamburlo, Valérie Buchillier, Henry Markram, Henry Markram   +10 more
doaj   +1 more source

Automated multi-slice extracellular and patch-clamp experiments using the WinLTP data acquisition system with automated perfusion control

, 2012
WinLTP is a data acquisition program for studying long-term potentiation (LTP) and other aspects of synaptic function. Earlier versions of WinLTP (J. Neurosci.
Collingridge, Graham L, Fitzjohn, Stephen M, Anderson, William W   +2 more
core   +1 more source