Results 91 to 100 of about 1,044,066 (265)

Measuring endogenous ICRAC and ORAI currents with the patch-clamp technique. [PDF]

, 2014
Although ICRAC and other store-operated currents are often analyzed by Ca(2+) imaging, whole-cell patch clamp, described here, is the preferred technique to analyze ICRAC whenever possible.
Holzmann, Christian, Alansary, Dalia, Kilch, Tatiana, Peinelt, Christine, Hoth, Markus, Lis, Annette   +5 more
core   +1 more source

PLOD1 Catalytic Activity Stabilizes ENO1 by Limiting FBXW7‐dependent Degradation to Promote Glycolysis and TMZ Resistance in Glioblastoma

Advanced Science, EarlyView.
PLOD1 interacts with ENO1 and limits its FBXW7‐dependent ubiquitination and proteasomal degradation through a mechanism requiring PLOD1 catalytic activity. ENO1 stabilization sustains glycolysis, promoting glioblastoma cell proliferation, migration, and temozolomide resistance.
Fen Xue, Dingkong Liang, Xin Chen, Fei Fan, Xiayun He   +4 more
wiley   +1 more source

Cost-Effective and Rapid Detection of Tetrodotoxin Using Indium Tin Oxide Electrodes via In Vitro Electrophysiology and Electrochemistry

Toxins
The real-time, cost-effective detection of marine toxins like tetrodotoxin (TTX) remains a significant challenge for the scientific community. Traditional methods, including cell-based assays (CBAs), high-performance liquid chromatography (HPLC), and ...
Naga Adithya Chandra Pandurangi, Manel M. Santafe, Angels Tudo, Nagihan Ozsoy, Fransesc X. Sureda, Mark L. Dallas, Ioanis Katakis   +6 more
doaj   +1 more source

Patch-clamp-Recordings

, 2018
Patch-clamp ...
Masayoshi Okada
core  

Modulation of Presynaptic Inhibitory Retinal Circuits for Retinal Ganglion Cell Neuroprotection in Glaucoma

Advanced Science, EarlyView.
Ocular hypertension induces early hyperactivity in inner retinal inhibitory circuitry before substantial retinal ganglion cell loss. Sustained pharmacological inhibition of GABAergic/glycinergic neurotransmission and Vgat‐targeted Lin28 expression suppress this hyperactivity and improve RGC survival without reducing intraocular pressure. These findings
Eunji Hong, Chen Lin, Wenjie Long, Ian Kim, Mengwen Xiong, Zuer Wu, Siyuan Rao, Qianbin Wang   +7 more
wiley   +1 more source

Multiscale Interfacial Engineering Enables Robust Reconstituted Wood

Advanced Science, EarlyView.
Conventional adhesives used in wood composites create heterogeneous interfaces that cause stress concentration under humid or aqueous conditions, thereby compromising long‐term stability. A synergistic strategy integrating interfacial reconstruction and water‐induced regeneration transforms heterogeneous interfaces into homogeneous ones with ...
Huizheng Wu, Yi Kong, Liangxian Liu, Yuyan Jiang, Jiahuan He, Haiyu Li, Ming Wei, Dexiu Min, Jian Li, Yanjun Xie, Chi Zhang, Shaoliang Xiao   +11 more
wiley   +1 more source

A novel methodology to characterize beat-to-beat alternations in whole-cell calcium currents.

PLoS ONE
An automated high-throughput procedure to quantify the degree of electrical beat-to-beat alternants in human atrial myocytes from electrophysiological recordings of the transmembrane ion currents of calcium ions (Ca2+) is presented and discussed.
Xavier Marimon, Miguel Cerrolaza, Carmen Tarifa, Leif Hove-Madsen   +3 more
doaj   +1 more source

Recording ten-fold larger IKr conductances with automated patch clamping using equimolar Cs+ solutions

Frontiers in Physiology
Background: The rapid delayed rectifier potassium current (IKr) is important for cardiac repolarization and is most often involved in drug-induced arrhythmias.
Meye Bloothooft, Bente Verbruggen, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Marcel A. G. van der Heyden, Niels Voigt, Niels Voigt, Niels Voigt, Teun P. de Boer   +10 more
doaj   +1 more source

Robotic automation of in vivo two photon targeted whole-cell patch clamp electrophysiology [PDF]

, 2017
Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed “blind,” meaning that throughput for ...
Agabi, OE, Copeland, Caroline, Morris, Alexander R, Schultz, SR, Simon R. Schultz, Caroline S. Copeland, Annecchino, Luca, Chadderton, Paul, Schultz, Simon, Agabi, Oshioroyena, Morris, AR, Chadderton, P, Luca A. Annecchino, Paul Chadderton, Annecchino, Luca A, Annecchino, LA, Agabi, Oshiorenoya E., Oshiorenoya E. Agabi, Copeland, Caroline S., Morris, Alexander, Alexander R. Morris, Schultz, Simon R, Copeland, CS   +22 more
core   +1 more source

Co‐Design Insights for Mechanically Active Flexible Printed Circuit Boards Through the CurveQuad Origami Robot

Advanced Science, EarlyView.
This paper presents the process of integrating electronics directly into the body sheet of CurveQuad, a small origami robot that can self‐fold, crawl, and steer with a single actuator. The authors design a flexible printed circuit board for the body sheet: the key challenge is to navigate how embedding electronics affects the mechanical properties and ...
Xuelin Wu, Daniel Feshbach, Satviki Vasireddy, Louis Beardell, Bao To, Yuliy Baryshnikov, Cynthia Sung   +6 more
wiley   +1 more source