Results 101 to 110 of about 1,044,066 (265)

Culturing and electrophysiology of cells on NRCC patch-clamp chips

, 2012
Due to its exquisite sensitivity and the ability to monitor and control individual cells at the level of ion channels, patch-clamping is the gold standard of electrophysiology applied to disease models and pharmaceutical screens alike 1.
Py, Christophe, Monette, Robert, Martina, Marzia, Luk, Collin, Mealing, Geoff, Comas, Tanya, Denhoff, Mike W., Syed, Naweed I.   +7 more
core   +1 more source

Human Engineered Heart Tissues Reproduce Trigger‐ and Substrate‐Driven Mechanisms of Ventricular Tachycardia

Advanced Science, EarlyView.
Engineered heart tissues (EHTs) derived from human iPSCs replicate mechanisms of ventricular tachycardia at the tissue level. Acquired Long‐QT conditions cause early afterdepolarizations, wavebreak, and transient rotors; in contrast, focal cryoinjury forms a stable substrate that supports ongoing reentry.
Matthew Fiedler, Alejandra Vasquez Limeta, Ernesto Reyes Sanchez, Leah Carter, Francisco Altamirano   +4 more
wiley   +1 more source

Structure and Function of a Multi‐Megadalton Virus‐Like Proteolytic Dodecahedron

Angewandte Chemie, EarlyView.
Zuzalysin, a virulence metallopeptidase of the periodontal pathogen Porphyromonas gingivalis, is secreted as a latent, flexible ≈95‐kDa monomer. Calcium triggers cysteine‐switch activation and hierarchical assembly into pentamers, bipentamers, tripentamers, and a ≈5.6‐MDa, ≈355‐Å dodecahedron. Structures at 1.8–3.6 Å reveal a virus‐sized catalytic cage
Mariusz Madej, Arturo Rodríguez‐Banqueri, Danuta Mizgalska, Borys Szmigielski, Zuzanna Nowakowska, Małgorzata Benedyk‐Machaczka, Monika Bzowska, Katarzyna Mikruta, Juan Sebastián Ramírez‐Larrota, Chinanu Agunanne, Olivier Julien, Anthony J. O'Donoghue, Carsten Scavenius, Mario López‐Martín, Enrique Marcos, Łukasz Koziej, Sebastian Glatt, Pablo Guerra, Ulrich Eckhard, Jan Potempa, F. Xavier Gomis‐Rüth   +20 more
wiley   +2 more sources

Automated in vivo patch-clamp evaluation of extracellular multielectrode array spike recording capability

, 2019
© 2018 American Physiological Society. All rights reserved. Much innovation is currently aimed at improving the number, density, and geometry of electrodes on extracellular multielectrode arrays for in vivo recording of neural activity in the mammalian ...
Tegmark, Max, Lamantia, Charlie, Seoane, Luís F, Boyden, Edward S, Kinney, Justin P, Scholvin, Jorg, Chronopoulos, Chris, Bernstein, Jacob G, Moore-Kochlacs, Caroline, Kodandaramaiah, Suhasa B, Allen, Brian D   +10 more
core   +1 more source

Propofol Modulates Fear Memory Reconsolidation by Inhibiting D2 Receptor‐Expressing Neurons in the Medial Prefrontal Cortex

Advanced Science, EarlyView.
This study shows that propofol modulates fear‐memory reconsolidation in mice. It works by suppressing D2 receptor‐expressing neurons within the medial prefrontal cortex and their connections to the basolateral amygdala. D2‐receptor agonists reproduce this fear‐reducing effect, offering potential non‐anesthetic therapeutic strategies for trauma‐related ...
Jie Li, Yi‐Ge Guo, Xing Xu, Gui‐Lin Meng, Han Li, Liu‐Wei Chu, Xin‐Yuan Chen, Xiao‐Yun Guo, Tao Xu, Ti‐Fei Yuan, Ji Hu, Xiao‐Na Zhu   +11 more
wiley   +1 more source

Multi-neuron intracellular recording in vivo via interacting autopatching robots

eLife, 2018
The activities of groups of neurons in a circuit or brain region are important for neuronal computations that contribute to behaviors and disease states.
Suhasa B Kodandaramaiah, Francisco J Flores, Gregory L Holst, Annabelle C Singer, Xue Han, Emery N Brown, Edward S Boyden, Craig R Forest   +7 more
doaj   +1 more source

ReLink: Reliable Deep Learning for Microscopic Motion Tracking

Advanced Science, EarlyView.
ReLink enables reliable trajectory linking in single‐particle tracking by integrating physics‐informed motion statistics with confidence‐based adaptive filtering. Across diverse simulated and experimental conditions, ReLink robustly reconstructs trajectories and preserves the fidelity of downstream analyses, enabling accurate characterization of ...
Yuan Jiang, Xiaochen Feng, Hongda Wei, Hao Sha, Hongpeng Wang, Min Zhang, Sanyang Han, Yongbing Zhang   +7 more
wiley   +1 more source

Microfluidic polymeric device for improved planar patch-clamp

, 2012
Ion channels are under-exploited membrane protein target class, due to the labour-intensive and low-throughput nature of traditional patch-clamp. Basic and clinical research require much efficiency, higher throughput and procedures simplification ...
Valbusa U, Firpo G, Repetto L, Guida P
core   +1 more source

Characterizing Biopolymer Electrolytes in Zinc–Air Batteries: Challenges, Best Practices, and a Robust Workflow Guiding Future Research Paths

Advanced Energy Materials, EarlyView.
Bio‐based gel polymer electrolytes promise sustainable, mechanically adaptable zinc–air batteries, yet their progress is constrained by inconsistent characterization. This review critically links formulation, structure, interfaces, and cell performance, identifies methodological gaps under alkaline operating conditions, and proposes application ...
Matteo Milanesi, Hiba Ali, Barbara Belza, Mattia F. Palermo, Claudio Gerbaldi, Giuseppe Antonio Elia, Nagore Ortiz‐Vitoriano   +6 more
wiley   +1 more source

Patch-Clamp Fluorometry: Electrophysiology meets Fluorescence

, 2014
Ion channels and transporters are membrane proteins whose functions are driven by conformational changes. Classical biophysical techniques provide insight into either the structure or the function of these proteins, but a full understanding of their ...
Kusch Jana, Zifarelli Giovanni
core   +1 more source