Results 231 to 240 of about 1,044,066 (265)

An Automated Planar Patch-Clamp Approach to Measure the Membrane Potential and Resting Membrane Currents in a Human Cerebrovascular Endothelial Cell Line

Journal of Neuroscience Methods
Background: The conventional “whole-cell patch-clamp” recording technique is widely used to measure the resting membrane potential (VM) and to dissect the underlying membrane ionic conductances in isolated vascular endothelial cells.
Giovambattista De Sarro, Teresa Soda, Francesco Moccia   +2 more
exaly   +2 more sources

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Microchip Technology for Automated and Parallel Patch‐Clamp Recording

Small, 2006
AbstractThe patch‐clamp technique is the state‐of‐the‐art technology for the study of a large class of membrane proteins called ion channels. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets.
Brüggemann, Andrea, Stoelzle-Feix, Sonja, George, Michael, Behrends, Jan C., Fertig, Niels   +4 more
openaire   +3 more sources

Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module [PDF]

SLAS Technology, 2016
We have developed an automated patch clamp module for high-throughput ion channel screening, recording from 384 cells simultaneously. The module is incorporated into a laboratory pipetting robot and uses a 384-channel pipettor head for application of cells and compounds.
Alison Obergrussberger, Niels Fertig, Andrea Bruggemann   +2 more
exaly   +3 more sources

Ion channel screening – automated patch clamp on the rise

Drug Discovery Today: Technologies, 2008
Ion channel proteins are of major importance for the human physiology and thus highly attractive molecular drug targets. Large-scale ion channel screening of wanted and unwanted drug effects is required, but has been limited by the lack of adequate screening technology, because available methods put a trade-off between high-throughput and high ...
Cecilia Farre, Michael George, Andrea Brüggemann, Niels Fertig   +3 more
openaire   +2 more sources

Automated ion channel screening: patch clamping made easy

Expert Opinion on Therapeutic Targets, 2007
Efficient high resolution techniques are required for screening efforts and research targeting ion channels. The conventional patch clamp technique, a high resolution but low efficiency technique, has been established for 25 years. Recent advances have opened up new possibilities for automated patch clamping.
Cecilia, Farre, Sonja, Stoelzle, Claudia, Haarmann, Michael, George, Andrea, Brüggemann, Niels, Fertig   +5 more
openaire   +2 more sources

Study of TRP Channels by Automated Patch Clamp Systems

2010
Ion channels are responsible for the permeation of ions across the membrane and their central role in cellular physiology is well established. Historically, the direct study of ion channels has been considered technically challenging. As such, a significant barrier to drug discovery for ion channels has been the low throughput of high quality ...
Morten, Sunesen, Rasmus B, Jacobsen
openaire   +2 more sources

Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology

2020
The automated patch clamp (APC) technology is used for increasing the data throughput of electrophysiological measurements, especially in safety pharmacology and drug discovery. Typically, electrical access to the cells are obtained using standard whole-cell formation by rupturing the membrane, thereby causing a rapid washout of cytosolic components ...
Kadla R, Rosholm, Kim, Boddum, Anders, Lindquist   +2 more
openaire   +2 more sources

Automated Patch-Clamp Methods for the hERG Cardiac Potassium Channel

2017
The human Ether-a-go-go Related Gene (hERG) product has been identified as a central ion channel underlying both familial forms of elongated QT interval on the electrocardiogram and drug-induced elongation of the same QT segment. Indeed, reduced function of this potassium channel involved in the repolarization of the cardiac action potential can ...
Sylvie, Houtmann, Brigitte, Schombert, Camille, Sanson, Michel, Partiseti, G Andrees, Bohme   +4 more
openaire   +2 more sources

A device for automated control of pipette internal pressure for patch-clamp recording

Journal of Neuroscience Methods, 2003
Formation of a high-resistance seal between the tip of a glass recording pipette and the membrane of the recorded cell is the crucial step in patch clamping, or whole cell recording with patch pipettes. Formation of the seal, and subsequent rupture of the membrane for whole cell recording, requires a specific sequence of changes in pipette internal ...
Philip M, Heyward, Michael T, Shipley
openaire   +2 more sources

Optimization of Cav1.2 screening with an automated planar patch clamp platform

Journal of Pharmacological and Toxicological Methods, 2009
Ca(v)1.2 channels play an important role in shaping the cardiac action potential. Screening pharmaceutical compounds for Ca(v)1.2 block is very important in developing drugs without cardiac liability. Ca(v)1.2 screening has been traditionally done using fluorescence assays, but these assays have some limitations.
Bharathi, Balasubramanian, John P, Imredy, David, Kim, Jacob, Penniman, Armando, Lagrutta, Joseph J, Salata   +5 more
openaire   +2 more sources