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Studying Mechanosensitive Ion Channels With an Automated Patch Clamp [PDF]
European Biophysics Journal, 2014 Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they could not be applied to MSCs.
Armagan Kocer, Jan P Birkner
exaly +5 more sources
Automated Whole-Cell Patch-Clamp Electrophysiology of Neurons in Vivo [PDF]
Nature Methods, 2012 Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal ...
Craig R Forest +2 more
exaly +7 more sources
Development of Automated Patch Clamp System for Electrophysiology
2013 IEEE International Conference on Robotics and Biomimetics (ROBIO), 2013 In this paper, the development of an automated patch clamp system for cellular electrical information is reported. The system involves several developments that enable the following tasks: “pipette and cell localization”, “pipette engaging”, “gigaseal”, and “break-in”.
Runhuai Yang, W C Lai
exaly +4 more sources
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A novel method for patch-clamp automation
Pflügers Archiv - European Journal of Physiology, 2006An increasing demand of the pharmaceutical industry for automated electrophysiological stations for ion channel drug discovery has recently resulted in the development of several commercial platforms for secondary and safety screening of ion channel modulators.
D, Vasilyev +4 more
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Optogenetics and Optical Tools in Automated Patch Clamping
2020Automated patch clamping (APC) has been used for almost two decades to increase the throughput of electrophysiological measurements, especially in preclinical safety screening of drug compounds. Typically, cells are suctioned onto holes in planar surfaces and a stronger subsequent suction allows access to a whole cell configuration for electrical ...
Kim, Boddum +3 more
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2013
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as ...
Carol J, Milligan, Clemens, Möller
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Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as ...
Carol J, Milligan, Clemens, Möller
openaire +2 more sources
The Chiptip: A Novel Tool for Automated Patch Clamp
Combinatorial Chemistry & High Throughput Screening, 2009To facilitate automated patch clamp measurements of ion channels in cells, the development of an all-glass Chiptip pipette is reported that may be combined with the previously described Flip-the-Tip technology. A single measurement requires less than 50 cells, and the addition of drugs for screening can be limited to very low volumes down to 1 microL ...
Albrecht, Lepple-Wienhues +5 more
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QPatch: The Past, Present and Future of Automated Patch Clamp
Expert Opinion on Therapeutic Targets, 2006The QPatch 16 significantly increases throughput for gigaseal patch clamp experiments, making direct measurements in ion channel drug discovery and safety testing feasible. Released to the market in the Autumn of 2004 by Sophion Bioscience, the QPatch originated from work done at NeuroSearch (Denmark) in the early days of automated patch clamp.
exaly +3 more sources
Automated Patch Clamping Using the QPatch
2009Whole-cell voltage clamp electrophysiology using glass patch pipettes (1) is regarded as the gold standard for measurement of compound activity on ion channels. Despite the high quality of the data generated by this method, in its traditional format, patch clamping has limited use in drug screening due to very low throughput.
Kenneth A, Jones +3 more
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Advances in the automation of whole-cell patch clamp technology
Journal of Neuroscience Methods, 2019Electrophysiology is the study of neural activity in the form of local field potentials, current flow through ion channels, calcium spikes, back propagating action potentials and somatic action potentials, all measurable on a millisecond timescale. Despite great progress in imaging technologies and sensor proteins, none of the currently available tools
Ho-Jun, Suk +2 more
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