Results 1 to 10 of about 1,007,449 (349)

A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag

eLife, 2022
Monitoring autophagic flux is necessary for most autophagy studies. The autophagic flux assays currently available for mammalian cells are generally complicated and do not yield highly quantitative results.
Willa Wen-You Yim, Hayashi Yamamoto, Noboru Mizushima   +2 more
doaj   +2 more sources

SIRT5‐Mediated Desuccinylation of RAB7A Protects Against Cadmium‐Induced Alzheimer's Disease‐Like Pathology by Restoring Autophagic Flux

Advanced Science
Cadmium (Cd) is a neurotoxic contaminant that induces cognitive decline similar to that observed in Alzheimer's disease (AD). Autophagic flux dysfunction is attributed to the pathogenesis of AD, and this study aimed to investigate the effect of autophagy
Ping Deng, Tengfei Fan, Peng Gao, Yongchun Peng, Min Li, Jingdian Li, Mingke Qin, Rongrong Hao, Liting Wang, Min Li, Lei Zhang, Chunhai Chen, Mindi He, Yonghui Lu, Qinlong Ma, Yan Luo, Li Tian, Jia Xie, Mengyan Chen, Shangcheng Xu, Zhou Zhou, Zhengping Yu, Huifeng Pi   +22 more
doaj   +2 more sources

S6K1 Is Indispensible for Stress-Induced Microtubule Acetylation and Autophagic Flux

Cells, 2021
Autophagy is a specific macromolecule and organelle degradation process. The target macromolecule or organelle is first enclosed in an autophagosome, and then delivered along acetylated microtubules to the lysosome.
Aleksandra Hać, Karolina Pierzynowska, Anna Herman-Antosiewicz   +2 more
doaj   +2 more sources

ATP6AP2 knockdown in cardiomyocyte deteriorates heart function via compromising autophagic flux and NLRP3 inflammasome activation

Cell Death Discovery, 2022
Moderate autophagy can remove damaged proteins and organelles. In some inflammatory diseases, autophagy plays a protective role by inhibiting the NOD-like receptor family pyrin domain containing 3(NLRP3).
Lei Li, Ya-juan Cui, Yu Liu, Hui-xin Li, Yu-dong Su, Sheng-nan Li, Lan-lan Wang, Yue-wen Zhao, Shuang-xi Wang, Feng Yan, Bo Dong   +10 more
doaj   +2 more sources

Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function

The EMBO Journal
Impaired autophagy is known to cause mitochondrial dysfunction and heart failure, in part due to altered mitophagy and protein quality control. However, whether additional mechanisms are involved in the development of mitochondrial dysfunction and heart ...
Quanjiang Zhang, Zhonggang Li, Qiuxia Li, Samuel AJ Trammell, Mark S Schmidt, Karla Maria Pires, Jinjin Cai, Yuan Zhang, Helena Kenny, Sihem Boudina, Charles Brenner, E Dale Abel   +11 more
doaj   +2 more sources

Autophagic flux modulates tumor heterogeneity and lineage plasticity in SCLC [PDF]

Frontiers in Oncology
IntroductionSmall cell lung cancer (SCLC) is characterized by significant heterogeneity and plasticity, contributing to its aggressive progression and therapy resistance.
Yujie Hao, Mingchen Li, Wenxu Liu, Zhenyi Ma, Zhe Liu, Zhe Liu, Zhe Liu   +6 more
doaj   +2 more sources

Lysosomal gene ATP6AP1 promotes doxorubicin resistance via up-regulating autophagic flux in breast cancer [PDF]

Cancer Cell International
Background Breast cancer remains the most prevalent malignancy in women. Chemotherapy is the primary systemic treatment modality, and the effectiveness of treatment is often hampered by chemoresistance.
Yinjiao Fei, Xueqin Yan, Mingxing Liang, Shu Zhou, Di Xu, Lei Li, Weilin Xu, Yuxin Song, Zhen Zhu, Jian Zhang   +9 more
doaj   +2 more sources

Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice [PDF]

Frontiers in Pharmacology
BackgroundThe increased myocardial vulnerability that occurs in diabetic patients following an ischemia-reperfusion injury (I/RI) represents a significant perioperative safety risk.
Jiyao Zhao, Wei Shi, Yan Zheng, Junjie Wang, Muzhao Yuan, Yultuz Anwar, Yuxuan He, Haiping Ma, Jianjiang Wu   +8 more
doaj   +2 more sources

Development of small fluorescent probes for the analysis of autophagy kinetics

iScience, 2023
Summary: Autophagy is a dynamic process that degrades subcellular constituents, and its activity is measured by autophagic flux. The tandem proteins RFP-GFP-LC3 and GFP-LC3-RFP-LC3ΔG, which enable the visualization of autophagic vacuoles of different ...
Hajime Tajima Sakurai, Hidefumi Iwashita, Satoko Arakawa, Alifu Yikelamu, Mizuki Kusaba, Satoshi Kofuji, Hiroshi Nishina, Munetaka Ishiyama, Yuichiro Ueno, Shigeomi Shimizu   +9 more
doaj   +1 more source

Activated Drp1 regulates p62-mediated autophagic flux and aggravates inflammation in cerebral ischemia-reperfusion via the ROS-RIP1/RIP3-exosome axis

Military Medical Research, 2022
Background Cerebral ischemia-reperfusion injury (CIRI) refers to a secondary brain injury that can occur when the blood supply to the ischemic brain tissue is restored. However, the mechanism underlying such injury remains elusive.
Xue Zeng, Yun-Dong Zhang, Ruiyan Ma, Yuanmei Chen, Xinming Xiang, D. Hou, Xuehan Li, He Huang, Tao Li, Chenggang Duan   +9 more
semanticscholar   +1 more source