Results 51 to 60 of about 81,092 (236)

Leishmania Antigen Nonspecific Binding to Avidin in Biotin-Avidin Immunoassays [PDF]

Clinical Diagnostic Laboratory Immunology, 2000
One of the most prevalent uses of avidin-biotin technology in recent years has been their use in immunoassays. In many cases, the signal is enhanced 10-fold or more, owing to the four biotin-binding sites of avidin and the multiple biotinyl groups on the derivatized antibody ([4][1], [5][2 ...
S, Agudelo, M, Portús
openaire   +2 more sources

TEAD1 Enhances Exosome Secretion and Promotes Exosome‐Mediated Tissue Regeneration

Advanced Science, EarlyView.
TEAD1 functions as a crucial molecular switch regulating exosome secretion in various cell types. TEAD1 enhances exosome secretion by upregulating key proteins associated with exosome secretion, including RAB11, CD9, and SNAP23. This study reveals a novel role for TEAD1 in regulating exosome secretion and tissue regeneration, particularly in diabetic ...
Yan Pu, Yi Wan, Wenhao Shi, Bin Li, Haiyue Zhang, Jun Wu, Lingling Li, Wenjia Guo, Chen Ding, Wenjun Yang   +9 more
wiley   +1 more source

Engineered GM1 Intersects Between Mitochondrial and Synaptic Pathways to Ameliorate ALS Pathology

Advanced Science, EarlyView.
Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease driven by genetic and molecular disruptions affecting energy balance, protein homeostasis, and stress responses in nerve cells. Studies using human and rodent models identified convergent defects in mitochondria and synaptic function.
Federica Pilotto, Tristan Dellazizzo Toth, Silvano Bond, Alexander Schmitz, Rim Diab, Sara Y. Ngo Tenlep, Brian Mooney, Silvia Erni, Martina Schobesberger, Olivier Scheidegger, Camille Peitsch, Smita Saxena   +11 more
wiley   +1 more source

Modular Assembly of Dynamic Polymer Networks From Heteroaffinity Cross‐Links to Multivalent Proteins

Angewandte Chemie, EarlyView.
This study develops a method to modularly assemble oligomeric binding proteins, like streptavidin, using heterobifunctional cross‐linkers that have both a strong‐ and a weak‐affinity binding moiety. These dynamic cross‐linkers enable the assembly of polymer networks with unique meta‐stable nonequilibrium topologies, and that exhibit chemically tunable ...
Tianyue Dai, Yuntao Qiu, Katherine M. Leon Hernandez, Wesley Chan, Katrina Mejia, Natalia Nemeria, Colin D. Kinz‐Thompson   +6 more
wiley   +2 more sources

Three-dimensional structures of avidin and the avidin-biotin complex. [PDF]

Proceedings of the National Academy of Sciences, 1993
The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exceptionally tight binding of biotin.
O, Livnah, E A, Bayer, M, Wilchek, J L, Sussman   +3 more
openaire   +2 more sources

A Tac1‐Expressing Brainstem Pathway Underlies the Pathogenesis of Trigeminal Neuralgia

Advanced Science, EarlyView.
A critical TG‐Sp5CTac1‐PBNTac1 pathway drives trigeminal neuropathic pain (TNP). Tac1‐expressing parabrachial nucleus (PBNTac1) neurons exhibit heightened responses to innocuous stimuli in TNP, and chemogenetic inhibition of these neurons effectively prevents TNP development.
Liting Sun, Jia‐Jia Wang, Xiang‐Yu Li, Xin‐Yi Lin, Juan Li, Qiu‐Tong Yu, Zi‐Han Wang, Xue‐Ping Gao, Lei Jin, Wei‐Ke Li, Tian‐Lin Cheng, Juan Deng   +11 more
wiley   +1 more source

Chemical linkage to injected tissues is a distinctive property of oxidized avidin.

PLoS ONE, 2011
We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups.
Rita De Santis, Anna Maria Anastasi, Angela Pelliccia, Antonio Rosi, Claudio Albertoni, Antonio Verdoliva, Fiorella Petronzelli, Valeria D'Alessio, Serenella Serani, Carlo Antonio Nuzzolo   +9 more
doaj   +1 more source

Deuterium Depletion Inhibits Cell Proliferation, RNA and Nuclear Membrane Turnover to Enhance Survival in Pancreatic Cancer

Cancer Control, 2021
The effects of deuterium-depleted water (DDW) containing deuterium (D) at a concentration of 25 parts per million (ppm), 50 ppm, 105 ppm and the control at 150 ppm were monitored in MIA-PaCa-2 pancreatic cancer cells by the real-time cell impedance ...
László G. Boros, Ildikó Somlyai, Beáta Zs. Kovács, László G. Puskás, Lajos I. Nagy, László Dux, Gyula Farkas, Gábor Somlyai   +7 more
doaj   +1 more source

Islet Amyloid Polypeptide Modelled to Simulate Diabetes Co‐Oligomerized with β‐Amyloid 1‐42 Reproducing the Pathological Cascade of Alzheimer's Disease in Human Cerebral Organoids

Advanced Science, EarlyView.
Human islet amyloid polypeptide (hIAPP) was used to mimic T2DM, and Aβ42‐hIAPP co‐oligomers were delivered into the human mature cerebral organoids (COs), which reproduce typical AD pathology and significant neuronal death more closely resembling that of AD patients.
Jin Yan, Zhimeng Tang, Yanyu Luo, Bin Liu, Xinxin Huang, Jun Wei, Feng Yue   +6 more
wiley   +1 more source

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

Biosensors
L-Lactate is an important bioanalyte in the food industry, biotechnology, and human healthcare. In this work, we report the development of a new L-lactate electrochemical biosensor based on the use of multiwalled carbon nanotubes non-covalently ...
Alejandro Tamborelli, Michael López Mujica, Marilla Amaranto, José Luis Barra, Gustavo Rivas, Agustina Godino, Pablo Dalmasso   +6 more
doaj   +1 more source