Results 31 to 40 of about 90,586 (267)

Avidin-GG coupling analysis.

open access: yes, 2019
(A) Elution analysis: Fluorescence intensity of supernatants of hydrogel samples immersed in PBS. Supernatant was collected from separate samples over 2 days and analyzed with fluorescence spectrometer.
Jenny Parraga (6755090)   +7 more
core   +1 more source

Selective Targeting of Proteins by Hybrid Polyoxometalates: Interaction Between a Bis-Biotinylated Hybrid Conjugate and Avidin

open access: yesFrontiers in Chemistry, 2018
The Keggin-type polyoxometalate [γ-SiW10O36]8− was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique were ...
Valeria A. Zamolo   +12 more
doaj   +1 more source

Conjugated Avidin Identifies Cutaneous Rodent and Human Mast Cells [PDF]

open access: yes, 1984
Avidin conjugated to the fluorescent dyes rhodamine or fluorescein binds to mast cell granules in rodent and human skin. Sequential staining of tissue mast cells first with conjugated avidin, and then with a metachromatic stain demonstrated that both ...
Tharp, Michael D.   +2 more
core   +1 more source

Generating a High Valency Biotin Binder by Selecting Uniform Protein Assemblies via Crystallization

open access: yesCrystals, 2019
Crystallization is a common practice in the purification process in small molecule synthesis while selecting the wanted product. For proteins it is rarely applied due to the methodological predicaments in obtaining crystals.
Orly Avraham   +2 more
doaj   +1 more source

Intraprocedural endothelial cell seeding of arterial stents via biotin/avidin targeting mitigates in-stent restenosis

open access: yesScientific Reports, 2022
Impaired endothelialization of endovascular stents has been established as a major cause of in-stent restenosis and late stent thrombosis. Attempts to enhance endothelialization of inner stent surfaces by pre-seeding the stents with endothelial cells in ...
Ivan S. Alferiev   +7 more
doaj   +1 more source

Biotin-Functionalized Semiconducting Polymer in an Organic Field Effect Transistor and Application as a Biosensor

open access: yesSensors, 2012
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor.
Yong Suk Yang   +4 more
doaj   +1 more source

Genetic and Imaging Approaches Reveal Pro-Inflammatory and Immunoregulatory Roles of Mast Cells in Contact Hypersensitivity

open access: yesFrontiers in Immunology, 2018
Contact hypersensitivity (CHS) is a common T cell-mediated skin disease induced by epicutaneous sensitization to haptens. Mast cells (MCs) are widely deployed in the skin and can be activated during CHS responses to secrete diverse products, including ...
Nicolas Gaudenzio   +7 more
doaj   +1 more source

Synthesis and In Vitro Cytotoxicity and Antibacterial Activity of Novel 1,2,3-Triazol-5-yl-Phosphonates

open access: yesMolecules, 2020
Novel 1,2,3-triazol-5-yl-phosphonates were prepared by the copper(I)-catalyzed domino reaction of phenylacetylene, organic azides and dialkyl phosphites.
Anna Tripolszky   +6 more
doaj   +1 more source

Novel Biopesticides Based on Recombinant Avidin for Protection of Crops Against Insect Pests [PDF]

open access: yes, 2012
Farmers are required to increase their food productivity to meet the demand from a continually enlarging population. A major constraint on meeting this requirement is the damage created by insect pests.
HINCHLIFFE, GARETH
core  

Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin.

open access: yesExperimental Biology and Medicine, 1964
SummaryAn assay method involving the use of radioactive biotin and Sephadex chromatography was used to determine avidin and/or the avidin-biotin complex. It was demonstrated that avidin is relatively heat labile while the avidin-biotin complex is relatively heat stable.
R D, WEI, L D, WRIGHT
openaire   +2 more sources

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