Results 31 to 40 of about 130,909 (260)

Allosteric Regulation Alters Carrier Domain Translocation in Pyruvate Carboxylase [PDF]

, 2018
Pyruvate carboxylase (PC) catalyzes the ATP-dependent carboxylation of pyruvate to oxaloacetate. The reaction occurs in two separate catalytic domains, coupled by the long-range translocation of a biotinylated carrier domain (BCCP). Here, we use a series
Budelier, Melissa M., Liu, Yumeng, St. Maurice, Martin, Stine, Katelyn   +3 more
core   +3 more sources

Mutations in human dynamin block an intermediate stage in coated vesicle formation [PDF]

, 1993
The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to ...
Damke, Hanna, Meyerowitz, Elliot M., Redelmeier, Thomas E., Schmid, Sandra L., Tisdale, Ellen J., van der Bliek, Alexander M.   +5 more
core   +3 more sources

The determination of avidin in genetically modified maize by voltammetric techniques

Plant, Soil and Environment, 2007
Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities.
J. Petrlová, S. Křížková, V. Šupálková, M. Masařík, V. Adam, L. Havel, K.J. Kramer, R. Kizek   +7 more
doaj   +1 more source

Intraprocedural endothelial cell seeding of arterial stents via biotin/avidin targeting mitigates in-stent restenosis

Scientific Reports, 2022
Impaired endothelialization of endovascular stents has been established as a major cause of in-stent restenosis and late stent thrombosis. Attempts to enhance endothelialization of inner stent surfaces by pre-seeding the stents with endothelial cells in ...
Ivan S. Alferiev, Bahman Hooshdaran, Benjamin B. Pressly, Philip W. Zoltick, Stanley J. Stachelek, Michael Chorny, Robert J. Levy, Ilia Fishbein   +7 more
doaj   +1 more source

Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]

, 2012
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Agnew, Heather D., Beauchamp, J. L., Czerwieniec, Gregg, Deshaies, Raymond J., Graham, Robert L. J., Heath, James R., Hess, Sonja, Lee, J. Eugene, Loo, Joseph A., Smith, Geoffrey T., Sohn, Chang Ho, Sweredoski, Michael J.   +11 more
core   +2 more sources

Bifunctional avidin with covalently modifiable ligand binding site. [PDF]

PLoS ONE, 2011
The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins.
Jenni Leppiniemi, Juha A E Määttä, Henrik Hammaren, Mikko Soikkeli, Mikko Laitaoja, Janne Jänis, Markku S Kulomaa, Vesa P Hytönen   +7 more
doaj   +1 more source

Monoreactive high affinity and polyreactive low affinity rheumatoid factors are produced by CD5+ B cells from patients with rheumatoid arthritis. [PDF]

, 1988
In patients with rheumatoid arthritis (RA), circulating CD5+ B lymphocytes, but not CD5- B lymphocytes, are increased in number and size, exist in an activated state, spontaneously proliferate, and secrete Ig that binds to the Fc fragment of IgG.
Burastero, SE, Casali, P, Notkins, AL, Wilder, RL   +3 more
core   +1 more source

Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum

Biosensors, 2012
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the ...
Mathieu Lazerges, Hubert Perrot, Niriniony Rabehagasoa, Chantal Compère   +3 more
doaj   +1 more source

Synthesis and In Vitro Cytotoxicity and Antibacterial Activity of Novel 1,2,3-Triazol-5-yl-Phosphonates

Molecules, 2020
Novel 1,2,3-triazol-5-yl-phosphonates were prepared by the copper(I)-catalyzed domino reaction of phenylacetylene, organic azides and dialkyl phosphites.
Anna Tripolszky, Emese Tóth, Pál Tamás Szabó, László Hackler, Beáta Kari, László G. Puskás, Erika Bálint   +6 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source