Selective Targeting of Proteins by Hybrid Polyoxometalates: Interaction Between a Bis-Biotinylated Hybrid Conjugate and Avidin [PDF]
Frontiers in Chemistry, 2018 The Keggin-type polyoxometalate [γ-SiW10O36]8− was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique were ...
Valeria A. Zamolo +12 more
doaj +4 more sources
A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk [PDF]
, 2016 Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed for serological ...
Brocchi, Emiliana +7 more
core +9 more sources
Protein-mediated dethreading of a biotin-functionalised pseudorotaxane [PDF]
, 2014 In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques.
Caldwell, S.T. +11 more
core +3 more sources
Grating-Coupled Surface Plasmon Resonance (GC-SPR) Optimization for Phase-Interrogation Biosensing in a Microfluidic Chamber. [PDF]
, 2018 Surface Plasmon Resonance (SPR)-based sensors have the advantage of being label-free, enzyme-free and real-time. However, their spreading in multidisciplinary research is still mostly limited to prism-coupled devices.
Borile, Giulia +4 more
core +5 more sources
pH-Mediated Regulation of Polymer Transport Through SiN Pores [PDF]
, 2018 We characterize the pH controlled polymer capture and transport thorough silicon nitride (SiN) pores subject to protonation. A charge regulation model able to reproduce the experimental zeta potential of SiN pores is coupled with electrohydrodynamic ...
Ala-Nissila, Tapio, Buyukdagli, Sahin
core +2 more sources
Molecules, 2016 Avidin is a tetrameric protein that belongs to the calycin superfamily. It has been studied mainly because of its extraordinary affinity to biotin, which led to a wide range of applications based on the avidin-biotin system.
Paweł Strzelczyk +3 more
doaj +1 more source
Facile synthesis of 1H-imidazo[1,2-b]pyrazoles via a sequential one-pot synthetic approach
Beilstein Journal of Organic Chemistry, 2014 5-Aminopyrazole-4-carbonitrile and ethyl 5-aminopyrazole-4-carboxylate, as potential trifunctional building blocks are introduced in a facile, chemo- and regioselective multicomponent assembly of imidazo[1,2-b]pyrazoles via the Groebke–Blackburn–Bienaymé
András Demjén +4 more
doaj +1 more source
Biology Open, 2018 Avidin is a key egg white antimicrobial protein with strong binding capacity for biotin, an essential growth and immune cell precursor. As such, it is assumed to have a pronounced, though still poorly explored, effect on hatchling phenotype.
Eva Krkavcová +4 more
doaj +1 more source
Comparative study of osteogenic activity of multilayers made of synthetic and biogenic polyelectrolytes [PDF]
, 2017 Polyelectrolyte multilayer (PEM) coatings on biomaterials are applied to tailor adhesion, growth, and function of cells on biomedical implants. Here, biogenic and synthetic polyelectrolytes (PEL) are used for layer-by-layer assembly to study the ...
Gonzalez-Garcia, Cristina +4 more
core +1 more source
Modular Assembly of Dynamic Polymer Networks From Heteroaffinity Cross-Links to Multivalent Proteins. [PDF]
Angew Chem Int Ed EnglThis study develops a method to modularly assemble oligomeric binding proteins, like streptavidin, using heterobifunctional cross‐linkers that have both a strong‐ and a weak‐affinity binding moiety. These dynamic cross‐linkers enable the assembly of polymer networks with unique meta‐stable nonequilibrium topologies, and that exhibit chemically tunable ...
Dai T +6 more
europepmc +3 more sources