Results 1 to 10 of about 90,586 (267)

Construction of chimeric dual-chain avidin by tandem fusion of the related avidins. [PDF]

PLoS ONE, 2011
BACKGROUND: Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this strong interaction.
Tiina A Riihimäki, Sampo Kukkurainen, Suvi Varjonen, Jarno Hörhä, Thomas K M Nyholm, Markku S Kulomaa, Vesa P Hytönen   +6 more
doaj   +9 more sources

Avidin related protein 2 shows unique structural and functional features among the avidin protein family

BMC Biotechnology, 2005
Background The chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a property that is utilized in ...
Salminen Tiina A, Johnson Mark S, Nyholm Thomas KM, Kulomaa Tuomas, Hörhä Jarno, Halling Katrin K, Kidron Heidi, Määttä Juha AE, Hytönen Vesa P, Kulomaa Markku S, Airenne Tomi T   +10 more
doaj   +2 more sources

Bifunctional avidin with covalently modifiable ligand binding site. [PDF]

PLoS ONE, 2011
The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins.
Jenni Leppiniemi, Juha A E Määttä, Henrik Hammaren, Mikko Soikkeli, Mikko Laitaoja, Janne Jänis, Markku S Kulomaa, Vesa P Hytönen   +7 more
doaj   +3 more sources

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish. [PDF]

FEBS Open Bio
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Saralahti AK, Ojanen MJT, Parikka M, Kauko O, Rämet M, Hytönen VP.   +5 more
europepmc   +2 more sources

The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation

Nanomaterials, 2021
The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the
Yanchao Lyu, Álvaro Martínez, Federica D’Incà, Fabrizio Mancin, Paolo Scrimin   +4 more
doaj   +1 more source

An Analysis of the Biotin–(Strept)avidin System in Immunoassays: Interference and Mitigation Strategies

Current Issues in Molecular Biology, 2023
An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer, Christopher B. Whitehurst   +1 more
doaj   +1 more source

Pedestal High-Contrast Gratings for Biosensing

Nanomaterials, 2022
High-contrast gratings (HCG) are an excellent candidate for label-free detection of various kinds of biomarkers because they exhibit sharp and sensitive optical resonances.
Leonid Yu. Beliaev, Peter Groth Stounbjerg, Giovanni Finco, Ada-Ioana Bunea, Radu Malureanu, Lars René Lindvold, Osamu Takayama, Peter E. Andersen, Andrei V. Lavrinenko   +8 more
doaj   +1 more source

Artificial Organelles with Orthogonal‐Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes

Advanced Science, 2021
The challenge of effective integration and use of artificial organelles with orthogonal‐responsive membranes and their communication in eukaryotic protocells is to understand the intrinsic membrane characteristics.
Xueyi Wang, Silvia Moreno, Susanne Boye, Peng Wang, Xiaoling Liu, Albena Lederer, Brigitte Voit, Dietmar Appelhans   +7 more
doaj   +1 more source

Avidin-Conjugated Nanofibrillar Cellulose Hydrogel Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts

, 2021
The future success of physiologically relevant three-dimensional (3D) cell/tissue models is dependent on the development of functional biomaterials, which can provide a well-defined 3D environment instructing cellular behavior. To establish a platform to
Markus Nuopponen, Piia Mikkonen, Vesa P. Hytönen (1337616), Vesa P. Hytönen, Piia Mikkonen (463694), Markus Nuopponen (2654836), Paula Turkki, Jenni Leppiniemi (166920), Alexander Dulebo (129151), Alexander Dulebo, Jenni Leppiniemi, Paula Turkki (265568), Zeeshan Mutahir, Zeeshan Mutahir (9148474)   +13 more
core   +1 more source

ROLE OF BIOTIN IN IMPROVING HATCHABILITY AND PHYSIOLOGICAL STATUS OF TURKEY CHICKS AS AFFECTED BY AGE OF FLOCK [PDF]

Egyptian Poultry Science, 2017
An experiment was conducted to study the effects of flock age and biotin injection at different times of incubation on hatchability and physiological status of newly hatched chicks.
mahmoud mahmoud
doaj   +1 more source